Method development for a quantitative analysis performed without any standard using an evaporative light-scattering detector

2007 ◽  
Vol 1161 (1-2) ◽  
pp. 152-156 ◽  
Author(s):  
Sylvie Heron ◽  
Marie-Geneviève Maloumbi ◽  
Michel Dreux ◽  
Eric Verette ◽  
Alain Tchapla
2013 ◽  
Vol 29 (1) ◽  
pp. 43-51 ◽  
Author(s):  
U. Gianfranco Spizzirri ◽  
Donatella Restuccia ◽  
Manuela Curcio ◽  
Ortensia I. Parisi ◽  
Francesca Iemma ◽  
...  

1999 ◽  
Vol 71 (10) ◽  
pp. 1983-1991 ◽  
Author(s):  
D. Berner ◽  
A. Dieffenbacher

The development, by collaborative study, of standardised method for the determination of mono- and diacylglycerols in vegetable oils and fats is described. The method involves separation of mono- and diacylglycerols by normal phase high-performance liquid-liquid chromatography (HPLC) and evaporative light scattering detection of a solution of oil, fat or a commercial mono- and diacylglycerol preparation in a organic solvent.


Author(s):  
Xiaoyong Zhang ◽  
Xuezhao Chen ◽  
Juan Jin ◽  
Minghua Gong ◽  
Qiang He ◽  
...  

Abstract Capilliposide B (CPS-B) and Capilliposide C (CPS-C), as the key components in Lysimachia capillipes Hemsl., increasingly aroused the interest and research concern of many researchers due to the good bioactivities. Nowadays, the reference standards of CPS-B and CPS-C yield were very limited. Due to the deficit of reference standards, the determination could be difficult to carry out, and the quality control and evaluation would be restrained afterwards. To solve this urgent problem, a quantitative analysis of multi-components by single-marker (QAMS) method was proposed and established based on high-performance liquid-chromatography tandem evaporative light-scattering detector. In this QAMS method, the content of the two bioactive components could be calculated by buddlejasaponin IV, which is applied as an external standard and readily obtained. And the methodological experiments were evaluated and indicated accuracy, stability and feasibility of this QAMS method. Therefore, in this study, this built method would properly meet the requirement of determination of CPS-B, CPS-C and quality control of the L. capillipes Hemsl. plant.


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