Wheat gluten amino acid composition analysis by high-performance anion-exchange chromatography with integrated pulsed amperometric detection

2009 ◽  
Vol 1216 (29) ◽  
pp. 5557-5562 ◽  
Author(s):  
Ine Rombouts ◽  
Lieve Lamberts ◽  
Inge Celus ◽  
Bert Lagrain ◽  
Kristof Brijs ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Composition ◽  
High Performance ◽  
Wheat Gluten ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Composition Analysis ◽  
Pulsed Amperometric Detection ◽  
Integrated Pulsed Amperometric Detection

Carbohydrate Analysis of Bradyrhizobial (NC 92) Lipopolysaccharides by High Performance-Anion Exchange Chromatography with Pulsed Amperometric Detection

1999 ◽  
Vol 19 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Hasi Das ◽  
Vani Jayaraman ◽  
Indranil Bhattacharya
Keyword(s):  
Anion Exchange ◽  
High Performance ◽  
Gel Filtration ◽  
Amperometric Detection ◽  
Anion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Composition Analysis ◽  
Pulsed Amperometric Detection ◽  
Acid Hydrolysate ◽  
Sepharose 4B

Composition analysis of monosaccharides of Sepharose 4B purified NC 92 LPS and the polysaccharides fractions from Sephadex G-50 chromatography was performed by high performance anion exchange chromatography using pulsed amperometric detection. Rhamnose, mannose, galactose and glucose are present in a substantial amount in the purified LPS (Pk I). High molecular weight purified polysaccharides (PS I) obtained after sephadex gel filtration of the purified LPS (Pk I) acid hydrolysate showed an increase in glucose:galactose ratio. This indicates the presence of the peanut root lectin (PRA II) specific sugar in higher proportion on the O-antigen part of the LPS molecule, which may aid in the critical recognition reaction.

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