Preparation of styrene-co-4-vinylpyridine magnetic polymer beads by microwave irradiation for analysis of trace 24-epibrassinolide in plant samples using high performance liquid chromatography

2010 ◽  
Vol 1217 (42) ◽  
pp. 6455-6461 ◽  
Author(s):  
Zhuomin Zhang ◽  
Yi Zhang ◽  
Wei Tan ◽  
Gongke Li ◽  
Yuling Hu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Microwave Irradiation ◽  
High Performance ◽  
Polymer Beads ◽  
Plant Samples

scholarly journals Quantitative Determination of Flavonoids by Column High-Performance Liquid Chromatography with Mass Spectrometry and Ultraviolet Absorption Detection in Artemisia afra and Comparative Studies with Various Species of Artemisia Plants

2009 ◽  
Vol 92 (2) ◽  
pp. 633-644 ◽  
Author(s):  
Bharathi Avula ◽  
Yan-Hong Wang ◽  
Troy J Smillie ◽  
Wilfred Mabusela ◽  
Leszek Vincent ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
High Performance ◽  
Reversed Phase ◽  
Charge Ratio ◽  
Array Detector ◽  
Plant Samples

Abstract A simple and specific analytical method for the quantitative determination of flavonoids from the aerial parts of the Artemisia afra plant samples was developed. By column high-performance liquid chromatography (HPLC) with UV absorption and mass spectrometry (MS) detection, separation was achieved on a reversed-phase octadecylsilyl (C18) column with water, methanol, and acetonitrile, all containing 0.1 acetic acid, as the mobile phase. These methods were used to analyze various species of Artemisia plant samples. The wavelength used for quantification of flavonoids with the diode array detector was 335 nm. The limits of detection (LOD) by HPLC/MS were found to be 7.5, 7.5, 10, 2.0, and 2.0 ng/mL; and by LC-UV the LODs were 500, 500, 500, 300, and 300 ng/mL for apigenin, chrysoeriol, tamarixetin, acacetin, and genkwanin, respectively. The HPLC/MS method was found to be 50150 times more sensitive than the HPLC-UV method. HPLC/MS coupled with an electrospray ionization interface is described for the identification and quantification of flavonoids in various plant samples. This method involved the use of the MH<sup/> ions of the compounds at mass-to-charge ratio of 1.0606, 301.0712, 317.0661, 285.0763, and 285.0763 (calculated mass), respectively, in the positive ion mode with extractive ion monitoring.

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