The separation of flavonoids from Pongamia pinnata using combination columns in high-speed counter-current chromatography with a three-phase solvent system

2013 ◽  
Vol 1315 ◽  
pp. 80-85 ◽  
Author(s):  
Hao Yin ◽  
Si Zhang ◽  
Lijuan Long ◽  
Hang Yin ◽  
Xinpeng Tian ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Pongamia Pinnata ◽  
Three Phase ◽  
Counter Current

scholarly journals Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 332
Author(s):  
Ye Yuan ◽  
Xiaoping He ◽  
Tingting Wang ◽  
Xingwang Zhang ◽  
Zhong Li ◽  
...  
Keyword(s):  
Crude Extract ◽  
High Speed ◽  
Solvent System ◽  
Bafilomycin A1 ◽  
Middle Phase ◽  
Two Phase ◽  
Rapid Separation ◽  
Lower Phase ◽  
Three Phase ◽  
Counter Current

An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–acetonitrile–water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane–acetonitrile–water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract.

Liquid–liquid/solid three-phase high-speed counter-current chromatography based on O-carboxymethyl chitosan-functionalized multi-walled carbon nanotubes as solvent additive

RSC Advances ◽  
2014 ◽  
Vol 4 (50) ◽  
pp. 26231-26239 ◽  
Author(s):  
Yanjuan Liu ◽  
Yongfeng Liu ◽  
Gaohong Wang ◽  
Duolong Di
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Carboxymethyl Chitosan ◽  
System P ◽  
Three Phase ◽  
Multi Walled Carbon Nanotubes ◽  
Carbon Nano Tubes ◽  
Solvent Additive ◽  
Walled Carbon Nanotubes ◽  
Counter Current

A new liquid–liquid/solid three phase HSCCC system was established based on O-carboxymethyl chitosan-functionalized multi-walled carbon nano tubes as additive of biphasic solvent system.

Comprehensive separation of secondary metabolites in natural products by high-speed counter-current chromatography using a three-phase solvent system

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 74-81 ◽  
Author(s):  
Akio Yanagida ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Ako Oda ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
High Speed ◽  
Solvent System ◽  
Three Phase ◽  
Counter Current

Liquid-liquid/solid three-phase high-speed counter-current chromatography, a new technique for separation of polyphenols from Geranium wilfordii Maxim

2012 ◽  
Vol 35 (16) ◽  
pp. 2146-2151 ◽  
Author(s):  
Dan Liu ◽  
Yan Ma ◽  
Ming Gu ◽  
Jan-Christer Janson ◽  
Changhai Wang ◽  
...  
Keyword(s):  
High Speed ◽  
New Technique ◽  
Three Phase ◽  
A New Technique ◽  
Counter Current

scholarly journals Quick Preparative Separation of Natural Naphthopyranones with Antioxidant Activity by High-Speed Counter-Current Chromatography

2002 ◽  
Vol 57 (11-12) ◽  
pp. 1051-1055 ◽  
Author(s):  
Gilda G. Leitão ◽  
Suzana G. Leitão ◽  
Wagner Vilegas
Keyword(s):  
Antioxidant Activity ◽  
Ethyl Acetate ◽  
High Speed ◽  
Ethanolic Extract ◽  
Solvent System ◽  
Spectrophotometric Assay ◽  
Preparative Separation ◽  
Dpph Radical ◽  
Preparative Scale ◽  
Counter Current

The natural naphthopyranones paepalantine (1), paepalantine-9O-β-ᴅ-glucopyranoside (2) and paepalantine-9-O-β-ᴅ-allopyranosyl-(1→6)-O-β-ᴅ-glucopyranoside (3) were separated in a preparative scale from the ethanolic extract of the capitula of Paepalanthus bromelioides by high-speed counter-current chromatography (HSCCC). The solvent system used was composed of water-ethanol-ethyl acetate-hexane (10:4 : 10:4, v/v/v/v). This technique led to the separation of the three different naphthopyranone glycosides in pure form in approximately 7 hours. Paepalantine showed a good antioxidant activity when assayed by the DPPH radical spectrophotometric assay.

Three-phase solvent systems for comprehensive separation of a wide variety of compounds by high-speed counter-current chromatography

2006 ◽  
Vol 1133 (1-2) ◽  
pp. 119-125 ◽  
Author(s):  
Yoichi Shibusawa ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Akio Yanagida ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
High Speed ◽  
Three Phase ◽  
Solvent Systems ◽  
Counter Current
Sign in / Sign up

Export Citation Format

Share Document