A Three-Phase Solvent System in High-Speed Counter-Current Chromatographic for the Separation and Purification of Bioactive Constituents from Acanthus ilicifolius

2015 ◽  
Vol 78 (21-22) ◽  
pp. 1401-1407 ◽  
Author(s):  
Fazuo Wang ◽  
Ru Li ◽  
Lijuan Long ◽  
Xinpeng Tian ◽  
Zhihui Xiao ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Separation And Purification ◽  
Bioactive Constituents ◽  
Acanthus Ilicifolius ◽  
Three Phase ◽  
Counter Current

Use of O-carboxymethyl chitosan in high-speed counter-current chromatography: a novel additive for a biphasic solvent system

2014 ◽  
Vol 38 (3) ◽  
pp. 1150-1157 ◽  
Author(s):  
Yanjuan Liu ◽  
Gaohong Wang ◽  
Xinyi Huang ◽  
Yongfeng Liu ◽  
Duolong Di
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Carboxymethyl Chitosan ◽  
Separation And Purification ◽  
Active Constituents ◽  
System P ◽  
Natural Plants ◽  
Counter Current

A novel additive,O-carboxymethyl chitosan, for high-speed counter-current chromatography was found and evaluated for the separation and purification of active constituents from natural plants.

scholarly journals Efficient Preparation of Bafilomycin A1 from Marine Streptomyces lohii Fermentation Using Three-Phase Extraction and High-Speed Counter-Current Chromatography

Marine Drugs ◽  
2020 ◽  
Vol 18 (6) ◽  
pp. 332
Author(s):  
Ye Yuan ◽  
Xiaoping He ◽  
Tingting Wang ◽  
Xingwang Zhang ◽  
Zhong Li ◽  
...  
Keyword(s):  
Crude Extract ◽  
High Speed ◽  
Solvent System ◽  
Bafilomycin A1 ◽  
Middle Phase ◽  
Two Phase ◽  
Rapid Separation ◽  
Lower Phase ◽  
Three Phase ◽  
Counter Current

An efficient strategy was developed for the rapid separation and enrichment of bafilomycin A1 (baf A1) from a crude extract of the marine microorganism Streptomyces lohii fermentation. This strategy comprises liquid−liquid extraction (LLE) with a three-phase solvent system (n-hexane–ethyl acetate–acetonitrile–water = 7:3:5:5, v/v/v/v) followed by separation using high-speed counter-current chromatography (HSCCC). The results showed that a 480.2-mg fraction of baf A1-enriched extract in the middle phase of the three-phase solvent system was prepared from 4.9 g of crude extract after two consecutive one-step operations. Over 99% of soybean oil, the main hydrophobic waste in the crude extract, and the majority of hydrophilic impurities were distributed in the upper and lower phase, respectively. HSCCC was used with a two-phase solvent system composed of n-hexane–acetonitrile–water (15:8:12, v/v/v) to isolate and purify baf A1 from the middle phase fraction, which yielded 77.4 mg of baf A1 with > 95% purity within 90 min. The overall recovery of baf A1 in the process was determined to be 95.7%. The use of a three-phase solvent system represents a novel strategy for the simultaneous removal of hydrophobic oil and hydrophilic impurities from a microbial fermentation extract.

Preparative Separation and Purification of Lancifodilactone C from Schisandra Chinensis (Turcz.) Baill by High-Speed Counter-Current Chromatography

2013 ◽  
Vol 634-638 ◽  
pp. 1502-1505
Author(s):  
Bin Li ◽  
Xian Jun Meng ◽  
Li Jie Zhu ◽  
Xin Yao Jiao
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Butyl Alcohol ◽  
Structure Identification ◽  
Schisandra Chinensis ◽  
Preparative Separation ◽  
Separation And Purification ◽  
Two Phase ◽  
H Nmr ◽  
Counter Current

High-speed counter-current chromatography (HSCCC) was successfully applied to the preparative separation and purification of lancifodilactone C from the crude extracts of Schisandra chinensis (Turcz.) Baill. Following an initial cleaning-up step on the AB-8 macroporous resin, a preparative high-speed counter-current chromatography (HSCCC) with a two-phase solvent system composed of Chloroform- n-Butyl alcohol- methanol -water(10:0.5:8:4，v/v) was used to isolate and separate lancifodilactone C from Schisandra Chinensis(Turcz.) Baill. A total of 101 mg lancifodilactone C with purities of 98.2% were obtained from 1000 mg crude extract in one-step elution and less than 1 h, and the structure identification was performed by UV, IR, MS,1H NMR and13C NMR.

Liquid–liquid/solid three-phase high-speed counter-current chromatography based on O-carboxymethyl chitosan-functionalized multi-walled carbon nanotubes as solvent additive

RSC Advances ◽  
2014 ◽  
Vol 4 (50) ◽  
pp. 26231-26239 ◽  
Author(s):  
Yanjuan Liu ◽  
Yongfeng Liu ◽  
Gaohong Wang ◽  
Duolong Di
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Carboxymethyl Chitosan ◽  
System P ◽  
Three Phase ◽  
Multi Walled Carbon Nanotubes ◽  
Carbon Nano Tubes ◽  
Solvent Additive ◽  
Walled Carbon Nanotubes ◽  
Counter Current

A new liquid–liquid/solid three phase HSCCC system was established based on O-carboxymethyl chitosan-functionalized multi-walled carbon nano tubes as additive of biphasic solvent system.

Advances in Separation and Purification of Bioactive Polysaccharides through High-speed Counter-Current Chromatography

2020 ◽  
Vol 58 (10) ◽  
pp. 992-1000
Author(s):  
Yu Yang ◽  
Bilal Muhammad Khan ◽  
Xiping Zhang ◽  
Yongjie Zhao ◽  
Kit-Leong Cheong ◽  
...  
Keyword(s):  
Natural Products ◽  
High Speed ◽  
Solvent System ◽  
Future Research ◽  
Phase System ◽  
Separation And Purification ◽  
Two Phase ◽  
Bioactive Substances ◽  
Efficient System ◽  
Counter Current

Abstract Polysaccharides, with an extensive distribution in natural products, represent a group of natural bioactive substances having widespread applications in health-care food products and as biomaterials. Devising an efficient system for the separation and purification of polysaccharides from natural sources, hence, is of utmost importance in the widespread applicability and feasibility of research for the development of polysaccharide-based products. High-speed counter-current chromatography (HSCCC) is a continuous liquid–liquid partitioning chromatography with the ability to support a high loading amount and crude material treatment. Due to its flexible two-phase solvent system, HSCCC has been successfully used in the separation of many natural products. Based on HSCCC unique advantages over general column chromatography and its enhanced superiority in this regard when coupled to aqueous two-phase system (ATPS), this review summarizes the separation and purification of various bioactive polysaccharides through HSCCC and its coupling to ATPS as an aid in future research in this direction.

Comprehensive separation of secondary metabolites in natural products by high-speed counter-current chromatography using a three-phase solvent system

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 74-81 ◽  
Author(s):  
Akio Yanagida ◽  
Yutaka Yamakawa ◽  
Ryoko Noji ◽  
Ako Oda ◽  
Heisaburo Shindo ◽  
...  
Keyword(s):  
Natural Products ◽  
Secondary Metabolites ◽  
High Speed ◽  
Solvent System ◽  
Three Phase ◽  
Counter Current

High-Purity Isolation of Trans-Resveratrol from Rhizma Polygoni Cuspidati by High-Speed Counter-Current Chromatography

2013 ◽  
Vol 690-693 ◽  
pp. 1335-1339
Author(s):  
Jian Chao Deng ◽  
Lai Hao Li ◽  
Xian Qing Yang ◽  
Su Ping Lu ◽  
Yan Yan Wu ◽  
...  
Keyword(s):  
High Speed ◽  
Solvent System ◽  
Separation And Purification ◽  
Two Phase ◽  
Dry Extract ◽  
H Nmr ◽  
Assisted Extraction ◽  
Solvent Systems ◽  
Counter Current ◽  
Chloroform Methanol

In this paper, high-speed counter-current chromatography (HSCCC) was established and successfully applied for the separation and purification of trans-resveratrol from Rhizma Polygoni Cuspidati. After extracted by microwave assisted extraction (MAE), the dry extract was directly dissolved in HSCCC solvent and introduced to HSCCC separation system. Two-phase solvent system of HSCCC was optimized. Trans-resveratrol was separated from extract of Rhizma Polygoni Cuspidati by high-speed counter-current chromatography in two-step using two different solvent systems composed of chloroform-methanol-water (4:3:2, v/v/v) and n-hexane-ethyl acetate-methanol-water (3:5:4:6, v/v/v/v). From 200 mg of crude extract, 2.5 mg of trans-resveratrol was obtained at over 99% purity by HPLC analyses, and its chemical structure was identified by ESI-MS, 1H NMR.

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