Serological and molecular detection of Theileria equi in sport horses of northeastern Brazil

Abstract Recently, the importance of wild-living rodents for maintenance of pathogens of the family Anaplasmataceae in the environment was investigated. These mammals play a role as reservoirs for these pathogens and act as hosts for the immature stages of tick vectors. The aim of the present study was to investigate the prevalence of Ehrlichia sp. and Anaplasma sp. in 24 specimens of Azara’s agouti (Dasyprocta azarae) that had been trapped in the Itapiracó Environmental Reserve, in São Luís, Maranhão, northeastern Brazil, using molecular methods. Four animals (16.7%) were positive for Ehrlichia spp. in nested PCR assays based on the 16S rRNA gene. In a phylogenetic analysis based on the 16S rRNA gene, using the maximum likelihood method and the GTRGAMMA+I evolutionary model, Ehrlichia sp. genotypes detected in Azara’s agoutis were found to be closely related to E. canis and to genotypes relating to E. canis that had previously been detected in free-living animals in Brazil. The present work showed the first molecular detection of Ehrlichia sp. in Azara’s agoutis in Brazil.

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Molecular detection and prevalence of Theileria equi and Babesia caballi in horses of central Balkan

Acta Parasitologica ◽

10.1515/ap-2016-0044 ◽

2016 ◽

Vol 61 (2) ◽

Cited By ~ 7

Author(s):

Darko Davitkov ◽

Milos Vucicevic ◽

Jevrosima Stevanovic ◽

Vanja Krstic ◽

Dajana Slijepcevic ◽

...

Keyword(s):

Molecular Detection ◽

Wide Distribution ◽

Specific Region ◽

Theileria Equi ◽

Species Determination ◽

Babesia Caballi ◽

Equine Piroplasmosis ◽

Pcr Products ◽

Tick Borne Disease ◽

Insight Into

AbstractEquine piroplasmosis is significant tick-borne disease with wide distribution. The prevalence of equine piroplasmosis in Serbia, Montenegro and Bosnia and Herzegovina is unknown. In aim to obtain a first insight into the prevalence we performed molecular epidemiological study which included 142 horses, on seven locations in these three countries. We first performed PCR for the detection of a 450bp long section of the 18S rRNA of piroplasma-specific region. For all positive samples we have done multiplex PCR for the species detection. Species determination was further confirmed by sequencing PCR products of 10 randomly selected

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Ehrlichia canis in dogs in a semiarid region of Northeastern Brazil: Serology, molecular detection and associated factors

Research in Veterinary Science ◽

10.1016/j.rvsc.2012.10.007 ◽

2013 ◽

Vol 94 (3) ◽

pp. 474-477 ◽

Cited By ~ 13

Author(s):

A. Tanikawa ◽

M.B. Labruna ◽

A. Costa ◽

D.M. Aguiar ◽

S.V. Justiniano ◽

...

Keyword(s):

Associated Factors ◽

Molecular Detection ◽

Ehrlichia Canis ◽

Northeastern Brazil ◽

Semiarid Region

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MOLECULAR DETECTION OF Leishmania IN PHLEBOTOMINE SAND FLIES IN A CUTANEOUS AND VISCERAL LEISHMANIASIS ENDEMIC AREA IN NORTHEASTERN BRAZIL

Revista do Instituto de Medicina Tropical de São Paulo ◽

10.1590/s0036-46652014000400015 ◽

2014 ◽

Vol 56 (4) ◽

pp. 357-360 ◽

Cited By ~ 5

Author(s):

Vanessa Cristina Fitipaldi Veloso Guimarães ◽

Pietra Lemos Costa ◽

Fernando José da Silva ◽

Fábio Lopes de Melo ◽

Filipe Dantas-Torres ◽

...

Keyword(s):

Molecular Detection ◽

Northeastern Brazil ◽

Sand Fly ◽

Sand Flies ◽

Phlebotomine Sand Flies ◽

Future Studies ◽

Specific Pcr ◽

Pcr Targeting ◽

First Time ◽

Genus Leishmania

Several phlebotomine sand fly species have been regarded as putative or proven vectors of parasites of the genus Leishmania in Brazil, but data for the northeastern region remains incipient. In this study, a total of 600 phlebotomine sand flies were grouped in pools of 10 specimens each and tested by a Leishmania genus-specific PCR and by a PCR targeting Leishmania (Leishmania) infantum. Fourteen out of 60 pools were positive by the genus-specific PCR, being five pools of L. migonei, seven of L. complexa, one of L. sordellii and one of L. naftalekatzi, which correspond to a minimal infection rate of 2.3% (14/600). Our results, associated with their known anthropophily and their abundance, suggest the participation of L. migonei and L. complexa as vectors of Leishmania in northeastern Brazil. Remarkably, this is the first time in this country that the detection of Leishmania DNA in L. sordellii and L. naftalekatzi has been reported, but future studies are necessary to better understand the significance of these findings.

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