Diversity and interaction of common bacterial blight disease-causing bacteria (Xanthomonas spp.) with Phaseolus vulgaris L.

Breeding for resistance is a major component in the integrated management of common bacterial disease of beans (Phaseolus vulgaris L.). Use of less virulent strains or strains with attenuated virulence may lead to selection of resistant genotypes with intermediate response, when exposed to more virulent strains of the pathogen. The objective of this study to identify and characterise Ugandan isolates of common bacterial blight disease-causing pathogens for virulence. Bacteria were isolated from leaf samples collected from districts of Kabale, Masaka, Bukomansimbi, Mubende, Mbale, Bulambuli and Apac, all in Uganda, during the first season of 2016. The bacteria were tested for pathogenicity, as well as virulence on both breeding and local varieties. The study identified three most virulent isolates, namely MBL020, KAB-3 and BUL-14, all belonging to Xathomonas citri pv fuscans. These isolates are very similar to those previously identified from Uganda (NCPB 670 and NCCPB 1402) more than 50 years ago. The study further revealed that NAROBEAN1, NAROBEAN 2, NAROBEAN 4, VAX 3, VAX5 and NE 2- 14- 8 had better resistance compared to other tested genotypes. Key words: Phaseolus vulgaris, Uganda, virulent strains

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A Radiation-induced Mutant with Resistance to Common Bacterial Blight Disease in Common Beans

HortScience ◽

10.21273/hortsci.30.3.577 ◽

1995 ◽

Vol 30 (3) ◽

pp. 577-578 ◽

Cited By ~ 2

Author(s):

Mohamed F. Mohamed ◽

Dermot P. Coyne ◽

Paul E. Read

Keyword(s):

Phaseolus Vulgaris ◽

Dominican Republic ◽

Bacterial Blight ◽

Xanthomonas Campestris ◽

Common Bacterial Blight ◽

Common Beans ◽

Radiation Induced ◽

Bacterial Blight Disease ◽

Blight Disease ◽

Induced Mutant

The leaf reaction of the Phaseolus vulgaris L. germplasm—UNECA (M6 mutant derived from the cultivar Chimbolito, Costa Rica), `Chimbolito', BAC-6 (Brazil), XAN-159 (Centro Internacional de Agricultura Tropical, Cali, Colombia), and `PC-50' (Domican Republic)—to Xanthomonas campestris pv. phaseoli strain V4S1 (Dominican Republic) were determined in two replicated trials conducted in a greenhouse in Lincoln, Neb. (Feb.–Mar. and July–Aug. 1993). `PC-50' and `Chimbolito' were susceptible to Xcp strain V4S1 in both tests. UNECA, BAC-6, and XAN-159 had similar levels of resistance to Xcp in the July to August trial. However, in the February to March trial, the resistance of UNECA was greater than that of BAC-6 but less than that of XAN-159.

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Identification of RAPD markers linked to common bacterial blight resistance genes in Phaseolus vulgaris L.

Genome ◽

10.1139/g97-071 ◽

1997 ◽

Vol 40 (4) ◽

pp. 544-551 ◽

Cited By ~ 25

Author(s):

Yonghe Bai ◽

T. E. Michaels ◽

K. P. Pauls

Keyword(s):

Phaseolus Vulgaris ◽

Linkage Group ◽

Bacterial Blight ◽

Rapd Markers ◽

Interspecific Cross ◽

Breeding Population ◽

Common Bacterial Blight ◽

Linkage Groups ◽

Phaseolus Vulgaris L ◽

Total Contribution

Seven hundred and fifty-six random primers were screened with bulks of genomic DNA from common bacterial blight (CBB) resistant and susceptible bean plants. The plants were from a breeding population derived from an interspecific cross between Phaseolus acutifolius and Phaseolus vulgaris. Four RAPD markers, named R7313, RE416, RE49, and R4865, were found to be significantly associated with CBB resistance in this population. Forty-nine molecular markers segregating in the population were clustered into 8 linkage groups by a MAPMAKER linkage analysis. The largest linkage group was 140 cM long and contained 25 marker loci, including marker R4865. Markers R7313, RE416, and RE49 were clustered on another linkage group. A regression analysis indicated that the markers in these two groups together accounted for 81% of the variation in CBB resistance in the population. The addition of another marker, M56810, which was not individually associated with CBB resistance, increased the total contribution to the trait to 87%.Key words: Phaseolus vulgaris L., common bacterial blight (CBB), polymerase chain reaction (PCR), RAPD markers, linkage groups.

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Comparison of Seed Transmission and Survival of Xanthomonas axonopodis pv. phaseoli and Xanthomonas fuscans subsp. fuscans in Common Bean Seeds

Plant Health Progress ◽

10.1094/php-2013-0923-01-br ◽

2013 ◽

Vol 14 (1) ◽

pp. 41 ◽

Cited By ~ 1

Author(s):

Y. He ◽

G. P. Munkvold

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Bacterial Blight ◽

Disease Incidence ◽

Seed Transmission ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Transmission Frequency ◽

Xanthomonas Axonopodis ◽

Seed Health

Xanthomonas axonopodis pv. phaseoli (Smith) Vauterin (Xap) and Xanthomonas fuscans subsp. fuscans Schaad et al. (Xff) cause indistinguishable symptoms known as common bacterial blight of bean (Phaseolus vulgaris L.). These results confirm a higher disease incidence and seed transmission frequency of Xff compared to Xap and reinforce the need for seed health tests that can differentiate the two species. Accepted for publication 21 August 2013. Published 23 September 2013.

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Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.)

Canadian Journal of Plant Science ◽

10.4141/cjps-2014-399 ◽

2015 ◽

Vol 95 (4) ◽

pp. 703-710 ◽

Cited By ~ 4

Author(s):

J. G. Boersma ◽

A. Hou ◽

C. L. Gillard ◽

K. B. McRae ◽

R. L. Conner

Keyword(s):

Phaseolus Vulgaris ◽

Seed Weight ◽

Bacterial Blight ◽

Common Bacterial Blight ◽

Yield Losses ◽

Dry Beans ◽

Phaseolus Vulgaris L ◽

Navy Bean ◽

Navy Beans ◽

The Impact

Boersma, J. G., Hou, A., Gillard, C. L., McRae, K. B. and Conner, R. L. 2015. Impact of common bacterial blight on the yield, seed weight and seed discoloration of different market classes of dry beans (Phaseolus vulgaris L.). Can. J. Plant Sci. 95: 703–710. Common bacterial blight (CBB) is a seed-borne disease of dry bean (Phaseolus vulgaris L.), causing significant economic loss to growers due to reductions in seed yield and quality and the need to annually purchase disease-free seed. Over the past decade a number of breeding lines and cultivars with resistance to CBB have been developed in several bean market classes including navy, black and cranberry beans. A comparison of three susceptible navy bean cultivars and seven resistant navy, three black and one cranberry bean entries in Manitoba revealed that most resistant navy and black bean lines had significantly reduced the incidence of leaf symptoms and their mean yield losses were reduced to less than 17%, while those of the susceptible lines were as high as 36% under severe disease pressure. Only the weakly resistant navy bean cultivar HR67 and the cranberry bean line F4GR1 failed to substantially reduce CBB symptoms or show a yield advantage. The Simple Sequence Repeat (SSR) marker PVctt001 in combination with Sequence Characterized Amplified Region (SCAR) marker SU91 was associated with a low incidence of CBB symptoms and a reduced yield loss in five navy bean lines, but not in the cranberry bean line F4GR1. Disease symptoms on the pods in the resistant black and navy beans and seed discoloration of navy beans caused by CBB were also significantly reduced by resistance. Seed weights were reduced by 2.1–4.7% in the susceptible cultivars, but there was only a slight or no decrease or no effect on the seed weight of the CBB-resistant lines and cultivars. Generally the magnitude of the reductions in yield was much greater than the impact on seed weight, which suggests that yield losses were caused by a combination of reduced seed weight and the number of seeds per plant.

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Mapping genetic factors affecting the reaction to Xanthomonas axonopodis pv. phaseoli in Phaseolus vulgaris L. under field conditions

Genome ◽

10.1139/g01-099 ◽

2001 ◽

Vol 44 (6) ◽

pp. 1046-1056 ◽

Cited By ~ 19

Author(s):

B Tar'an ◽

T E Michaels ◽

K P Pauls

Keyword(s):

Phaseolus Vulgaris ◽

Common Bean ◽

Length Polymorphism ◽

Bacterial Blight ◽

Genetic Factors ◽

Fragment Length Polymorphism ◽

Segregation Ratio ◽

Common Bacterial Blight ◽

Phaseolus Vulgaris L ◽

Xanthomonas Axonopodis

The objectives of the present study were to evaluate the field effects of Xanthomonas axonopodis pv. phaseoli (Xap), which causes common bacterial blight (CBB) on common bean (Phaseolus vulgaris L.), and to identify genetic factors for resistance to CBB using a linkage map constructed with random amplified polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP), simple sequence repeat (SSR), and amplified fragment length polymorphism (AFLP) markers. One hundred and forty-two F2:4 lines, derived from a cross between 'OAC Seaforth' and 'OAC 95-4', and the parents were evaluated for their field reaction to CBB. In the inoculated plots, the reaction to CBB was negatively correlated with seed yield, days to maturity, plant height, hypocotyl diameter, pods per plant, and harvest index. A reduction in seed yield and its components was observed when disease-free and CBB-inoculated plots were compared. The broad-sense heritability estimate of the reaction to CBB was 0.74. The disease segregation ratio was not significantly different from the expected segregation ratio for a single locus in an F2 generation. The major gene for CBB resistance was localized on linkage group (LG) G5. A simple interval mapping procedure identified three genomic regions associated with the reaction to CBB. One quantitative trait loci (QTL), each on LG G2 (BNG71DraI), G3 (BNG21EcoRV), and G5 (PHVPVPK-1) explained 36.3%, 10.2%, and 42.2% of the phenotypic variation for the reaction to CBB, respectively. Together, these loci explained 68.4% of the phenotypic variation. The relative positions of these QTL on the core common bean map and their comparison with the previous QTL for CBB resistance are discussed.Key words: common bean, molecular markers, common bacterial blight.

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