Effects of antioxidant supplementation on postprandial oxidative stress and endothelial dysfunction: A single-blind, 15-day clinical trial in patients with untreated type 2 diabetes, subjects with impaired glucose tolerance, and healthy controls

Objective: To evaluate the correlation of uric acid with oxidative stress and endothelial dysfunction in type 2 diabetic subjects.Material and Methods: The study included 120 subjects, among when 60 were type 2 diabetes subjects and the remaining 60 were age and gender matched healthy controls. The biochemical parameters, blood glucose, lipid profile, uric acid and homocysteine, were measured by standard kits in an autoanalyzer. Oxidative stress was evaluated by measuring malondialdehyde (MDA) and total antioxidant power by manual methods such as thio-barbituric acid reactive substances and ferric reducing ability of plasma (FRAP). Endothelial dysfunction was assessed by measuring nitric oxide (NO) by the kinetic cadmium method.Results: A significant elevation of triglycerides, low density lipoprotein (LDL), and MDA were observed in the type 2 diabetes mellitus patients while FRAP and NO were significantly reduced compared to the healthy controls. In addition, the uric acid levels had a highly significant correlation with FRAP (r=0.212, p-value=0.020), and moderately significant correlation with triglycerides (r=0.173, p-value=0.057) and homocysteine (r=0.178, p-value=0.051). Uric acid was negatively correlated with MDA and positively correlated with NO, but not statistically significant.Conclusion: Our findings suggest that uric acid may have antioxidant properties since it had a significant positive correlation with FRAP.

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Impact of exercise on diurnal and nocturnal markers of glycaemic variability and oxidative stress in obese individuals with type 2 diabetes or impaired glucose tolerance

Diabetes and Vascular Disease Research ◽

10.1177/1479164115579003 ◽

2015 ◽

Vol 12 (5) ◽

pp. 381-385 ◽

Cited By ~ 6

Author(s):

Sarah S Farabi ◽

David W Carley ◽

Donald Smith ◽

Lauretta Quinn

Keyword(s):

Oxidative Stress ◽

Type 2 Diabetes ◽

Glucose Tolerance ◽

Impaired Glucose Tolerance ◽

Glycaemic Variability ◽

And Oxidative Stress

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Serum levels of p53 and cytochrome c in subjects with type 2 diabetes and impaired glucose tolerance

Clinical & Investigative Medicine ◽

10.25011/cim.v32i4.6617 ◽

2009 ◽

Vol 32 (4) ◽

pp. 266 ◽

Cited By ~ 3

Author(s):

Yildiz Dincer ◽

Solen Himmetoglu ◽

Serap Yalin ◽

Taner Damci ◽

Hasan Ilkova ◽

...

Keyword(s):

Type 2 Diabetes ◽

Serum Level ◽

Cytochrome C ◽

Glucose Tolerance ◽

Impaired Glucose Tolerance ◽

Serum Levels ◽

Competitive Elisa ◽

Healthy Controls ◽

Significant Difference

Purpose: To examine apoptotic markers in serum of subjects with diabetes and impaired glucose tolerance (IGT). Serum levels of p53 and cytochrome c, regulator molecules for apoptosis, were measured in subjects with type 2 diabetes, subjects with IGT and healthy controls. Methods: Forty one subjects with type 2 diabetes, 27 with IGT and 27 healthy volunteers were included in the study. Serum level of cytochrome c and p53 were measured with competitive ELISA. Results: Serum levels of p53 were lower in the group of subjects with type 2 diabetes (085±0.39 U/ml) than in controls (1.09±0.49 U/ml) (P < 0.05) and in the subjects with IGT (0.98±0.37 U/ml) (P < 0.05). There was no significant difference between the group with IGT and controls. Also, there was no difference for serum level of cytochrome c among the groups. In the group of subjects with type 2 diabetes, serum level of cytochrome c was mildly correlated with HbA1c (r:0.39, P < 0.05). Conclusion: The present study shows that the serum level of p53 is lower in the patients with type 2 diabetes than in controls or in subjects with IGT. No difference was seen among the the groups for the serum level of cytochrome c.

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