Picking dynamic analysis for robotic harvesting of Agaricus bisporus mushrooms

2021 ◽  
Vol 185 ◽  
pp. 106145
Author(s):  
Mingsen Huang ◽  
Long He ◽  
Daeun Choi ◽  
John Pecchia ◽  
Yaoming Li
Author(s):  
K. S. McCarty ◽  
R. F. Weave ◽  
L. Kemper ◽  
F. S. Vogel

During the prodromal stages of sporulation in the Basidiomycete, Agaricus bisporus, mitochondria accumulate in the basidial cells, zygotes, in the gill tissues prior to entry of these mitochondria, together with two haploid nuclei and cytoplasmic ribosomes, into the exospores. The mitochondria contain prominent loci of DNA [Fig. 1]. A modified Kleinschmidt spread technique1 has been used to evaluate the DNA strands from purified whole mitochondria released by osmotic shock, mitochondrial DNA purified on CsCl gradients [density = 1.698 gms/cc], and DNA purified on ethidium bromide CsCl gradients. The DNA appeared as linear strands up to 25 u in length and circular forms 2.2-5.2 u in circumference. In specimens prepared by osmotic shock, many strands of DNA are apparently attached to membrane fragments [Fig. 2]. When mitochondria were ruptured in hypotonic sucrose and then fixed in glutaraldehyde, the ribosomes were released for electron microscopic examination.


2011 ◽  
Vol 131 (2) ◽  
pp. 166-170 ◽  
Author(s):  
Yoshihiro Nakata ◽  
Hiroshi Ishiguro ◽  
Katsuhiro Hirata

Author(s):  
Carlos Alberto Dutra Fraga Filho ◽  
Fernando César Meira Menandro ◽  
Rivânia Hermógenes Paulino de Romero ◽  
Juan Sérgio Romero Saenz

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