Molecular and phylogenetic analysis of a novel family of fibrinogen-related proteins from mosquito Aedes albopictus cell line

2008 ◽  
Vol 32 (5) ◽  
pp. 382-386 ◽  
Author(s):  
Rajnikant Dixit ◽  
Upal Roy ◽  
Millind S. Patole ◽  
Yogesh S. Shouche
Keyword(s):  
Phylogenetic Analysis ◽  
Cell Line ◽  
Aedes Albopictus ◽  
Albopictus Cell ◽  
Related Proteins

Transfection and colonization of Tetranychus truncatus Wolbachia strain wTtru in cell lines of the mosquito Aedes albopictus

2018 ◽  
Vol 23 (12) ◽  
pp. 2420 ◽  
Author(s):  
Xue Xia ◽  
Chang-Wu Peng ◽  
Yi-Jia Lu ◽  
Xiao-Ying Zheng ◽  
Xiao-Yue Hong
Keyword(s):  
Phylogenetic Analysis ◽  
Success Rate ◽  
Cell Line ◽  
Aedes Albopictus ◽  
Wolbachia Strain ◽  
Dynamic Changes ◽  
Endosymbiotic Bacteria ◽  
Production Practice ◽  
Albopictus Cell ◽  
The Relationship

Wolbachia are endosymbiotic bacteria that commonly infect arthropods and cause reproductive manipulations in hosts. Artificial transfection is one of the best methods for the study of Wolbachia-induced reproductive manipulations and its application in production practice. However, the low success rate of artificial transfection laid burden for investigation. The success rate of artificial transfection of Wolbachia between closely related hosts is usually higher than distant hosts, but the mechanism of transfection is unknown. In our study, the wTtru strain (supergroup B) infecting the Arachnida Tetranychus truncatus was transfected into the Aedes albopictus cell line (native-infected with wAlB strain from supergroup B). The wTtru strain was quantified and localized in the cell line to observe dynamic changes after transfection. A phylogenetic analysis of the relationship between the wTtru strain and the wAlB strain showed that the wTtru (ST=278) strain belonged to supergroup B and was closely related to the mosquitoes native Wolbachia wAlbB (ST=464), so we speculate that successful transfection was related to the Wolbachia strain, and furthermore, Wolbachia strain may be more important than host relationship. Our results provide a factual basis for studying artificial transfection of Wolbachia, and show that Wolbachia transfection between distant hosts can be achieved.

scholarly journals Mosquito densonucleosis viruses cause dramatically different infection phenotypes in the C6/36 Aedes albopictus cell line

Virology ◽  
2005 ◽  
Vol 337 (2) ◽  
pp. 253-261 ◽  
Author(s):  
Andrew Paterson ◽  
Erin Robinson ◽  
Erica Suchman ◽  
Boris Afanasiev ◽  
Jonathan Carlson
Keyword(s):  
Cell Line ◽  
Aedes Albopictus ◽  
Albopictus Cell

scholarly journals Proteomic analysis of an Aedes albopictus cell line infected with Dengue serotypes 1 and 3 viruses

2011 ◽  
Vol 4 (1) ◽  
pp. 138 ◽  
Author(s):  
Sirilaksana Patramool ◽  
Pornapat Surasombatpattana ◽  
Natthanej Luplertlop ◽  
Martial Sévéno ◽  
Valérie Choumet ◽  
...  
Keyword(s):  
Cell Line ◽  
Aedes Albopictus ◽  
Proteomic Analysis ◽  
Albopictus Cell

Infection, growth and maintenance of Wolbachia pipientis in clonal and non-clonal Aedes albopictus cell cultures

2012 ◽  
Vol 103 (3) ◽  
pp. 251-260 ◽  
Author(s):  
C.C.H. Khoo ◽  
C.M.P. Venard ◽  
Y. Fu ◽  
D.R. Mercer ◽  
S.L. Dobson
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Aedes Albopictus ◽  
Cell Types ◽  
Infection Level ◽  
Wolbachia Pipientis ◽  
Albopictus Cell ◽  
Host Cell Interactions ◽  
Insect Cell Lines

AbstractInsect cell lines provide useful in vitro models for studying biological systems, including interactions between mosquitoes and obligate intracellular endosymbionts such as Wolbachia pipientis. The Aedes albopictus Aa23 cell line was the first cell line developed to allow examination of Wolbachia infections. However, Wolbachia studies using Aa23 can be complicated by the presence of different cell types in the cell line and the substantial temporal variation in infection level. Two approaches were examined to ameliorate infection variability. In the first approach, multiple Aa23 passaging regimes were tested for an effect on infection variability. Fluorescence in situ hybridization (FISH) staining was used to characterize Wolbachia infection level over time. The results demonstrate an impact of passaging method on Wolbachia infection level, with some methods resulting in loss of infection. None of the passaging methods succeeded in effectively mitigating infection level variation. In a second approach, the clonal C7-10 A. albopictus cell line was infected with Wolbachia from Aa23 cells and Drosophila simulans (Riverside), resulting in cell lines designated C7-10B and C7-10R, respectively. Characterization via FISH staining showed greater stability and uniformity of Wolbachia infection in C7-10R relative to the infection in C7-10B. Characterization of the Aa23, C7-10B and C7-10R lines is discussed as a tool for the study of Wolbachia-host cell interactions.

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