Influence of application time on the establishment, survival, and ability of Clonostachys rosea to suppress Botrytis cinerea sporulation on rose debris

Reeh, K. W. and Cutler, G. C. 2013. Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry. Can. J. Plant Sci. 93: 639–642. Lowbush blueberry (Vaccinium angustifolium) is an economically important crop. Clonostachys rosea is an endophytic fungus that can provide protection of plants from several pathogens, including Botrytis cinerea. It is unknown if C. rosea is able to colonize and protect V. angustifolium, and whether it is tolerant of fungicides commonly used for B. cinerea management in blueberry production. In a greenhouse experiment, pre-treatment of blueberry blossoms with C. rosea significantly reduced establishment of B. cinerea, but treatments after establishment of the pathogen were not effective. Clonostachys rosea demonstrated in vitro tolerance to the fungicide Switch®, but little or no tolerance to Pristine® and Maestro®. Our results are encouraging for additional field research examining the use of C. rosea as part of an integrated pest management program for B. cinerea control on lowbush blueberries.

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Biological Control of Blossom Blight of Alfalfa Caused by Botrytis cinerea Under Environmentally Controlled and Field Conditions

Plant Disease ◽

10.1094/pdis.2004.88.11.1246 ◽

2004 ◽

Vol 88 (11) ◽

pp. 1246-1251 ◽

Cited By ~ 14

Author(s):

G. Q. Li ◽

H. C. Huang ◽

S. N. Acharya ◽

R. S. Erickson

Keyword(s):

Botrytis Cinerea ◽

Biocontrol Agent ◽

Development Stage ◽

Field Conditions ◽

Trichoderma Atroviride ◽

Clonostachys Rosea ◽

Pod Development ◽

Seed Rot ◽

Pod Development Stage

Fungal and bacterial antagonists were tested for their inhibition of sporulation of Botrytis cinerea on detached alfalfa florets. Clonostachys rosea, Gliocladium catenulatum, and Trichoderma atroviride were evaluated for protecting young blossoms and pods of alfalfa from infection by B. cinerea in vitro. C. rosea was further tested to control pod rot and seed rot caused by B. cinerea under field conditions. The results showed that four of the tested antagonists, C. rosea, G. catenulatum, T. atroviride, and Trichothecium roseum, could inhibit sporulation by B. cinerea on detached alfalfa florets. Both C. rosea and G. catenulatum were effective in suppression of infection of alfalfa pods by B. cinerea when inoculated on fresh petals of alfalfa at the anthesis stage, and their efficacy was greater than that of Trichoderma atroviride. A significant suppression of B. cinerea by C. rosea and G. catenulatum on pods and seed of alfalfa was observed when they were inoculated on senescent petals at the pod-development stage. Results of a field trial indicated that C. rosea applied to upper parts of alfalfa plants significantly suppressed pod rot and seed rot caused by B. cinerea, and significantly increased seed production of alfalfa in each of 3 years. These studies show that C. rosea has potential as a biocontrol agent for control of alfalfa blossom blight caused by B. cinerea.

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UV-B radiation reduces biocontrol ability of Clonostachys rosea against Botrytis cinerea

Biocontrol Science and Technology ◽

10.1080/09583157.2016.1241981 ◽

2016 ◽

Vol 26 (12) ◽

pp. 1736-1749 ◽

Cited By ~ 2

Author(s):

Lúcio Bertoldo Costa ◽

Marcelo Augusto Boechat Morandi ◽

Sara Marie Stricker ◽

Wagner Bettiol

Keyword(s):

Botrytis Cinerea ◽

Clonostachys Rosea

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Contrast Between Orange- and Black-Colored Sclerotial Isolates of Botrytis cinerea: Melanogenesis and Ecological Fitness

Plant Disease ◽

10.1094/pdis-11-16-1663-re ◽

2018 ◽

Vol 102 (2) ◽

pp. 428-436 ◽

Cited By ~ 7

Author(s):

Yingjun Zhou ◽

Na Li ◽

Jingyi Yang ◽

Long Yang ◽

Mingde Wu ◽

...

Keyword(s):

Botrytis Cinerea ◽

Soil Fungus ◽

Hubei Province ◽

Clonostachys Rosea ◽

Tomato Plants ◽

Ecological Fitness ◽

Agar Media ◽

First Time

Botrytis cinerea usually produces grayish mycelia and conidia as well as black-colored sclerotia (BS) due to accumulation of melanin. An isolate (XN-1) of B. cinerea producing orange-colored sclerotia (OS) on agar media was obtained from an orange-colored apothecium of an uncultured soil fungus. Whether or not the OS B. cinerea occurs on plants and how they differ from the BS isolates in melanogensis and ecological fitness remained unknown. This study, for the first time, confirmed the presence of the OS B. cinerea in strawberry and tomato plants that were surveyed in Hubei Province of China. Only five OS isolates were obtained from a total of 2,031 isolates surveyed from the two crops. The OS isolate XN-1 was compared and contrasted with the BS isolate B05.10 in sclerotial melanogenesis and ecological fitness. Sclerotial melanogenesis was evident in B05.10 but was deficient in XN-1. The OS were more susceptible to the two mycoparasites Trichoderma koningiopsis and Clonostachys rosea than the BS. The percentage of viable sclerotia after the mycoparasitism study was significantly (P < 0.01) lower in OS (21%) than in BS (48%). This study also reaffirmed the importance of melanization for survival of B. cinerea sclerotia.

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