scholarly journals Selection of Clonostachys rosea isolates from Brazilian ecosystems effective in controlling Botrytis cinerea

2005 ◽  
Vol 34 (2) ◽  
pp. 132-143 ◽  
Author(s):  
Sérgio A.M. Nobre ◽  
Luiz A. Maffia ◽  
Eduardo S.G. Mizubuti ◽  
Luciano V. Cota ◽  
Ana Paula S. Dias
Keyword(s):  
Botrytis Cinerea ◽  
Clonostachys Rosea ◽  
Selection Of

scholarly journals Fungicide resistance characterised across seven chemical classes in a Botrytis cinerea population isolated from Australian vineyards

2021 ◽  
Author(s):  
Lincoln A. Harper ◽  
Scott Paton ◽  
Barbara Hall ◽  
Suzanne McKay ◽  
Richard P. Oliver ◽  
...  
Keyword(s):  
Adverse Effect ◽  
Botrytis Cinerea ◽  
Fungicide Resistance ◽  
Chemical Control ◽  
Management Strategies ◽  
Resistance Management ◽  
Gray Mold ◽  
Chemical Groups ◽  
Important Disease ◽  
Selection Of

AbstractGray mold, caused by Botrytis cinerea, is an economically important disease of grapes in Australia and across grape growing regions worldwide. Control of this disease relies heavily on canopy management and the application of single site fungicides. Fungicide application can lead to the selection of fungicide resistant B. cinerea populations, which has an adverse effect on the chemical control of the disease. Characterising the distribution and severity of resistant B. cinerea populations is needed to inform resistance management strategies. In this study, 725 isolates were sampled from 75 Australian vineyards during 2013 – 2016 and were screened against seven fungicides with different MOAs. The resistance frequencies for azoxystrobin, boscalid, fenhexamid, fludioxonil, iprodione, pyrimethanil and tebuconazole were 5, 2.8, 2.1, 6.2, 11.6, 7.7 and 2.9% respectively. Nearly half of the resistant isolates (43.7%) were resistant to more than one of the fungicides tested. The frequency of vineyards with at least one isolate simultaneously resistant to 1, 2, 3, 4 or 5 fungicides was 19.5, 7.8, 6.5, 10.4 and 2.6%.Resistance was associated with previously published genotypes in CytB (G143A), SdhB (H272R/Y), Erg27 (F412S), Mrr1 (D354Y), Os1 (I365S, N373S + Q369P, I365S + D757N) and Pos5 (P319A, L412F). Expression analysis was used to characterise fludioxonil resistant isolates exhibiting overexpression (6.3 - 9.6-fold) of the ABC transporter encoded by AtrB (MDR1 phenotype). Novel genotypes were also described in Mrr1 (S611N, D616G) and Cyp51 (P357S). Resistance frequencies were lower when compared to most previously published surveys of both grape and non-grape B. cinerea resistance. Nonetheless, continued monitoring of critical chemical groups used in Australian vineyards is recommended.

Influence of application time on the establishment, survival, and ability of Clonostachys rosea to suppress Botrytis cinerea sporulation on rose debris

2008 ◽  
Vol 27 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Marcelo A.B. Morandi ◽  
Liliana P.V. Mattos ◽  
Elen R. Santos ◽  
Rafaella C. Bonugli
Keyword(s):  
Botrytis Cinerea ◽  
Application Time ◽  
Clonostachys Rosea

scholarly journals Baseline Sensitivity of Botrytis cinerea Isolates from Strawberry to Isofetamid Compared to other SDHIs

Plant Disease ◽  
2020 ◽  
Vol 104 (4) ◽  
pp. 1224-1230
Author(s):  
Adrian I. Zuniga ◽  
Michelle S. Oliveira ◽  
Carolina S. Rebello ◽  
Natalia A. Peres
Keyword(s):  
Botrytis Cinerea ◽  
Disease Incidence ◽  
The Other ◽  
Fruit Rot ◽  
Baseline Sensitivity ◽  
Sdhb Gene ◽  
Moderately Resistant ◽  
Selection Of ◽  
Succinate Dehydrogenase Inhibitors ◽  
Selection Of Resistance

Succinate dehydrogenase inhibitors (SDHIs) are the fungicides most commonly used to control Botrytis fruit rot on commercial strawberry in Florida. The medium-to-high risk of selection of resistance in the causal agent Botrytis cinerea is a threat to the efficacy of this fungicide group. In this study, we characterized the sensitivity of B. cinerea to the SDHI isofetamid, evaluated the SdhB gene mutation associated with resistance, and monitored resistance frequencies to five SDHI fungicides for two consecutive seasons. EC50 values of 70 isolates were obtained using the spiral gradient dilution (SGD) method and averaged 0.098 µg/ml of isofetamid. EC50 averages of 3.04 and >500.00 µg/ml were obtained for isolates with the N230I and P225F mutations indicating moderate and high resistance to isofetamid, respectively. A total of 565 B. cinerea isolates collected during 2015–2016 and 2016–2017 seasons from strawberry nurseries and Florida production fields were evaluated using conidial germination assays. Results for the first season showed resistance frequencies of 95, 33, 21, 25, and 0% to boscalid, penthiopyrad, fluopyram, benzovindiflupyr, and isofetamid, respectively. The respective resistance frequencies for the following season were 91, 95, 44, 27, and 1.3%. Only three isolates were found to be moderately resistant to isofetamid during the second season, and the mutation N230I was identified after sequence analysis. These isolates were confirmed to be resistant to isofetamid in fruit assays with disease incidence of 55.6 to 77.0%; however, the conidial production of the isolates was inhibited by an average of 83.9%. In general, isofetamid efficacy was higher than the other evaluated SDHIs, but a slight increase in resistance frequencies was observed in our study.

A multi-criteria approach for the selection of efficient biocontrol agents against Botrytis cinerea on tomato in Algeria

BioControl ◽  
2017 ◽  
Vol 63 (2) ◽  
pp. 299-311 ◽  
Author(s):  
Yousra Bouaoud ◽  
Claire Troulet ◽  
Abdelhamid Foughalia ◽  
Odile Berge ◽  
Kamel Aissat ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Biocontrol Agents ◽  
Selection Of

Insights into the molecular basis of biocontrol of Botrytis cinerea by Clonostachys rosea in tomato

2022 ◽  
Vol 291 ◽  
pp. 110547
Author(s):  
Fanyue Meng ◽  
Rui Lv ◽  
Mozhen Cheng ◽  
Fulei Mo ◽  
Nian Zhang ◽  
...  
Keyword(s):  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Clonostachys Rosea

scholarly journals Effects of UV-B radiation on the antagonistic ability of Clonostachys rosea to Botrytis cinerea on strawberry leaves

2013 ◽  
Vol 65 (1) ◽  
pp. 95-100 ◽  
Author(s):  
Lúcio B. Costa ◽  
Drauzio E.N. Rangel ◽  
Marcelo A.B. Morandi ◽  
Wagner Bettiol
Keyword(s):  
Botrytis Cinerea ◽  
Clonostachys Rosea

Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry

2013 ◽  
Vol 93 (4) ◽  
pp. 639-642 ◽  
Author(s):  
K. W. Reeh ◽  
G. C. Cutler
Keyword(s):  
Pest Management ◽  
Botrytis Cinerea ◽  
Endophytic Fungus ◽  
Field Research ◽  
Management Program ◽  
Clonostachys Rosea ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Pre Treatment

Reeh, K. W. and Cutler, G. C. 2013. Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry. Can. J. Plant Sci. 93: 639–642. Lowbush blueberry (Vaccinium angustifolium) is an economically important crop. Clonostachys rosea is an endophytic fungus that can provide protection of plants from several pathogens, including Botrytis cinerea. It is unknown if C. rosea is able to colonize and protect V. angustifolium, and whether it is tolerant of fungicides commonly used for B. cinerea management in blueberry production. In a greenhouse experiment, pre-treatment of blueberry blossoms with C. rosea significantly reduced establishment of B. cinerea, but treatments after establishment of the pathogen were not effective. Clonostachys rosea demonstrated in vitro tolerance to the fungicide Switch®, but little or no tolerance to Pristine® and Maestro®. Our results are encouraging for additional field research examining the use of C. rosea as part of an integrated pest management program for B. cinerea control on lowbush blueberries.

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