Ability of Clonostachys rosea to Establish and Suppress Sporulation Potential of Botrytis cinerea in Deleafed Stems of Hydroponic Greenhouse Tomatoes

Reeh, K. W. and Cutler, G. C. 2013. Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry. Can. J. Plant Sci. 93: 639–642. Lowbush blueberry (Vaccinium angustifolium) is an economically important crop. Clonostachys rosea is an endophytic fungus that can provide protection of plants from several pathogens, including Botrytis cinerea. It is unknown if C. rosea is able to colonize and protect V. angustifolium, and whether it is tolerant of fungicides commonly used for B. cinerea management in blueberry production. In a greenhouse experiment, pre-treatment of blueberry blossoms with C. rosea significantly reduced establishment of B. cinerea, but treatments after establishment of the pathogen were not effective. Clonostachys rosea demonstrated in vitro tolerance to the fungicide Switch®, but little or no tolerance to Pristine® and Maestro®. Our results are encouraging for additional field research examining the use of C. rosea as part of an integrated pest management program for B. cinerea control on lowbush blueberries.

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Biological control of stem canker of greenhouse tomatoes caused by Botrytis cinerea

Canadian Journal of Microbiology ◽

10.1139/w02-043 ◽

2002 ◽

Vol 48 (6) ◽

pp. 550-554 ◽

Cited By ~ 14

Author(s):

R S Utkhede ◽

S Mathur

Keyword(s):

Biological Control ◽

Botrytis Cinerea ◽

Stem Canker ◽

Fruit Yield ◽

The Other ◽

Post Inoculation ◽

Tomato Plants ◽

Grey Mold ◽

Rhodosporidium Diobovatum ◽

Greenhouse Tomatoes

Experiments were conducted to study the effect of various chemical and biological agents on stem canker caused by Botrytis cinerea Pers.: Fr. on tomato plants grown in sawdust under near-commercial greenhouse conditions. Lesion lengths following treatment with RootShield® and strain S33 of Rhodosporidium diobovatum Newell & Hunter, applied as post-inoculation sprays, were significantly smaller than those in inoculated controls. These treatments also increased fruit yield and decreased the number of dead plants compared with inoculated controls. Decree®, Prestop®, and R. diobovatum S33, applied as sprays, prevented the occurrence of stem canker and increased fruit yield in tomato. The number of dead plants was also smaller with these treatments than with the other treatments and in inoculated controls. These results suggest that, in tomato, post-inoculation sprays of RootShield® and R. diobovatum S33 can reduce lesion lengths, and that a preventive spray of Decree®, Prestop®, and R. diobovatum S33 might prevent stem canker, under near-commercial greenhouse conditions.Key words: biological control, Botrytis cinerea, Bacillus subtilis, Rhodosporidium diobovatum, grey mold.

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The influence of inoculum concentration, relative humidity, and temperature on infection of greenhouse tomatoes by Botrytis cinerea

Plant Pathology ◽

10.1046/j.1365-3059.1996.d01-163.x ◽

1996 ◽

Vol 45 (4) ◽

pp. 795-806 ◽

Cited By ~ 37

Author(s):

M. A. EDEN ◽

R. A. HILL ◽

R. BERESFORD ◽

A. STEWART

Keyword(s):

Relative Humidity ◽

Botrytis Cinerea ◽

Inoculum Concentration ◽

Greenhouse Tomatoes

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Biological Control of Blossom Blight of Alfalfa Caused by Botrytis cinerea Under Environmentally Controlled and Field Conditions

Plant Disease ◽

10.1094/pdis.2004.88.11.1246 ◽

2004 ◽

Vol 88 (11) ◽

pp. 1246-1251 ◽

Cited By ~ 14

Author(s):

G. Q. Li ◽

H. C. Huang ◽

S. N. Acharya ◽

R. S. Erickson

Keyword(s):

Botrytis Cinerea ◽

Biocontrol Agent ◽

Development Stage ◽

Field Conditions ◽

Trichoderma Atroviride ◽

Clonostachys Rosea ◽

Pod Development ◽

Seed Rot ◽

Pod Development Stage

Fungal and bacterial antagonists were tested for their inhibition of sporulation of Botrytis cinerea on detached alfalfa florets. Clonostachys rosea, Gliocladium catenulatum, and Trichoderma atroviride were evaluated for protecting young blossoms and pods of alfalfa from infection by B. cinerea in vitro. C. rosea was further tested to control pod rot and seed rot caused by B. cinerea under field conditions. The results showed that four of the tested antagonists, C. rosea, G. catenulatum, T. atroviride, and Trichothecium roseum, could inhibit sporulation by B. cinerea on detached alfalfa florets. Both C. rosea and G. catenulatum were effective in suppression of infection of alfalfa pods by B. cinerea when inoculated on fresh petals of alfalfa at the anthesis stage, and their efficacy was greater than that of Trichoderma atroviride. A significant suppression of B. cinerea by C. rosea and G. catenulatum on pods and seed of alfalfa was observed when they were inoculated on senescent petals at the pod-development stage. Results of a field trial indicated that C. rosea applied to upper parts of alfalfa plants significantly suppressed pod rot and seed rot caused by B. cinerea, and significantly increased seed production of alfalfa in each of 3 years. These studies show that C. rosea has potential as a biocontrol agent for control of alfalfa blossom blight caused by B. cinerea.

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