Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry

2013 ◽  
Vol 93 (4) ◽  
pp. 639-642 ◽  
Author(s):  
K. W. Reeh ◽  
G. C. Cutler
Keyword(s):  
Pest Management ◽  
Botrytis Cinerea ◽  
Endophytic Fungus ◽  
Field Research ◽  
Management Program ◽  
Clonostachys Rosea ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Pre Treatment

Reeh, K. W. and Cutler, G. C. 2013. Laboratory efficacy and fungicide compatibility of Clonostachys rosea against Botrytis blight on lowbush blueberry. Can. J. Plant Sci. 93: 639–642. Lowbush blueberry (Vaccinium angustifolium) is an economically important crop. Clonostachys rosea is an endophytic fungus that can provide protection of plants from several pathogens, including Botrytis cinerea. It is unknown if C. rosea is able to colonize and protect V. angustifolium, and whether it is tolerant of fungicides commonly used for B. cinerea management in blueberry production. In a greenhouse experiment, pre-treatment of blueberry blossoms with C. rosea significantly reduced establishment of B. cinerea, but treatments after establishment of the pathogen were not effective. Clonostachys rosea demonstrated in vitro tolerance to the fungicide Switch®, but little or no tolerance to Pristine® and Maestro®. Our results are encouraging for additional field research examining the use of C. rosea as part of an integrated pest management program for B. cinerea control on lowbush blueberries.

Vaccinium angustifolium (lowbush blueberry) leaf extract increases extravillous trophoblast cell migration and invasion in vitro

2018 ◽  
Vol 32 (4) ◽  
pp. 705-714 ◽  
Author(s):  
Christina Ly ◽  
Jonathan Ferrier ◽  
Jeremiah Gaudet ◽  
Julien Yockell-Lelièvre ◽  
John Thor Arnason ◽  
...  
Keyword(s):  
Cell Migration ◽  
Leaf Extract ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Migration And Invasion ◽  
Vaccinium Angustifolium ◽  
Cell Migration And Invasion ◽  
Lowbush Blueberry

DNA methylation in lowbush blueberry (Vaccinium angustifolium Ait.) propagated by softwood cutting and tissue culture

2018 ◽  
Vol 98 (5) ◽  
pp. 1035-1044 ◽  
Author(s):  
Juran C. Goyali ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Dna Methylation ◽  
Tissue Culture ◽  
Growth Conditions ◽  
Vaccinium Angustifolium ◽  
Lowbush Blueberry ◽  
Restriction Sites ◽  
Methylation Sensitive Amplification Polymorphism ◽  
Softwood Cutting

Plant DNA methylation is one of the frequent epigenetic variations induced by tissue culture. Global DNA methylation was evaluated in lowbush blueberry (Vaccinium angustifolium Ait.) wild clone QB9C and cultivar Fundy propagated by conventional softwood cutting (SC) and tissue culture (TC) using the methylation-sensitive amplification polymorphism (MSAP) technique. In all, 106 and 107 DNA fragments were amplified using 16 selective primer combinations in SC plants of QB9C and Fundy, respectively. In micropropagated QB9C and Fundy plants, there were 105 and 109 amplified fragments, respectively. Overall, 25% of restriction sites were methylated at the cytosine nucleotide in QB9C plants propagated by SC compared with 19% in Fundy. In contrast, a total of 29% and 20% of restriction sites were methylated at cytosine in micropropagated QB9C and Fundy plants, respectively. Tissue culture plants demonstrated higher methylation events than SC plants in both genotypes. Previously, methylation polymorphism has been detected in TC plants but not in SC counterparts. Different patterns of DNA methylation and polymorphism in the plants propagated in in vitro and in vivo conditions suggest the possibility of involvement of these fragments in the processes of regulating plant growth and development under prevailing growth conditions.

Morphology, phenolic content and antioxidant capacity of lowbush blueberry (Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods

2013 ◽  
Vol 93 (6) ◽  
pp. 1001-1008 ◽  
Author(s):  
Juran C. Goyali ◽  
Abir U. Igamberdiev ◽  
Samir C. Debnath
Keyword(s):  
Antioxidant Activity ◽  
Antioxidant Capacity ◽  
Phenolic Content ◽  
Flower Buds ◽  
Vaccinium Angustifolium ◽  
Growing Seasons ◽  
Lowbush Blueberry ◽  
Number Of Stems ◽  
Propagation Methods

Goyali, J. C., Igamberdiev, A. U. and Debnath, S. C. 2013. Morphology, phenolic content and antioxidant capacity of lowbush blueberry ( Vaccinium angustifolium Ait.) plants as affected by in vitro and ex vitro propagation methods. Can. J. Plant Sci. 93: 1001–1008. The lowbush blueberry (Vaccinium angustifolium Ait.), a commercially important crop in eastern Canada and the United States of America, is native to North America. It is one of the richest sources of antioxidant compounds and has been reported to be a potential component in reducing the incidence of cancers and cardiovascular diseases. The aim of this study is to evaluate the effect of propagation methods on morphological characters, phenolic content and antioxidant activity. A lowbush blueberry clone, QB 9C, and a cultivar, Fundy, were studied over two growing seasons after being propagated by conventional softwood cutting (SC) and by tissue culture (TC). Significant interactions among genotypes, propagation methods and growing seasons were observed for number of flower buds, total flavonoid (TFC) and proanthocyanidin (PAC) contents and antioxidant capacity. Propagation method interacted significantly with genotypes for the number of stems per plant and total phenolic (TPC) and chlorophyll contents, and with growing season for number of flower buds, TFC and PAC. TC plants produced higher number of stems and branches compared with SC plants. TPC, TFC, PAC, chlorophyll content and antioxidant activity were found in higher levels in the leaves of QB 9C compared with those of Fundy plants. The juvenile characteristics of TC plants which are triggered by growth hormones and readily available nutrients of culture media may be responsible for differences in morphological traits and antioxidant activity.

IN VITRO SHOOT PRODUCTION OF LOWBUSH BLUEBERRY

1983 ◽  
Vol 63 (2) ◽  
pp. 467-472 ◽  
Author(s):  
JOHN J. FRETT ◽  
JOHN M. SMAGULA
Keyword(s):  
Tissue Culture ◽  
Indoleacetic Acid ◽  
Vaccinium Angustifolium ◽  
Total Length ◽  
Lowbush Blueberry ◽  
Shoot Production ◽  
Shoot Number ◽  
Number Of Shoots

The six most distal buds of lowbush blueberry (Vaccinium angustifolium Ait.) from shoots previously developed in tissue culture were transferred to media with 0, 7.5, 15, 22.5 or 30 mg/L 2, 6 (γ,γ-dimethylallyl-amino)-purine (2ip) and 0, 2, 4, 6, or 8 mg/L indoleacetic acid (IAA). The positional origin of the bud explant had little effect on number of shoots and no effect on total length of shoots produced. Shoot number and total length of shoots per tube increased with increasing 2ip concentrations. Increasing IAA concentrations decreased shoot numbers but had no effect on total length of shoots. Shoots were removed from culture after 8 wk and rooted in a peat, vermiculite and perlite medium. Percent rooting increased with increasing 2ip concentration and decreased with increasing IAA concentration in the proliferation medium.Key words: Lowbush blueberry, Vaccinium angustifolium

scholarly journals Effect of Boron and Calcium Foliar Sprays on Pollen Germination and Development, Fruit Set, Seed Development, and Berry Yield and Quality in Lowbush Blueberry (Vaccinium angustifolium Ait.)

1998 ◽  
Vol 123 (4) ◽  
pp. 524-531 ◽  
Author(s):  
Youzhi Chen ◽  
John M. Smagula ◽  
Walter Litten ◽  
Scott Dunham
Keyword(s):  
Pollen Germination ◽  
Fruit Set ◽  
Yield Reduction ◽  
Vaccinium Angustifolium ◽  
Foliar Sprays ◽  
Lowbush Blueberry ◽  
Berry Size ◽  
Number Of Seeds

In a managed field of native Vaccinium angustifolium Ait. clones, the effect of fall foliar sprays of B at 345 g·ha-1 and/or Ca at 3,450 g·ha-1 in remedying tissue deficiency of B varied among 12 clones, as seen in pollen germinability and on individual stems as seen in flower number, fruit set, and number of harvestable berries. With Ca applied alone, increased berry size did not overcome yield reduction due to fewer flowers and berries per stem. Berry diameter and mass correlated better to number of seeds of germinable size than to total number of seeds. Pollen germination averaged 17.4% on stigmata from untreated clones, and all three treatments (B, Ca, B + Ca) increased that average by 8%. More seeds per berry with the B-alone treatment implies more ovules fertilized when B deficiency is remedied. No relation was found between in vitro and in vivo pollen germination.

scholarly journals Biological Control of Blossom Blight of Alfalfa Caused by Botrytis cinerea Under Environmentally Controlled and Field Conditions

Plant Disease ◽  
2004 ◽  
Vol 88 (11) ◽  
pp. 1246-1251 ◽  
Author(s):  
G. Q. Li ◽  
H. C. Huang ◽  
S. N. Acharya ◽  
R. S. Erickson
Keyword(s):  
Botrytis Cinerea ◽  
Biocontrol Agent ◽  
Development Stage ◽  
Field Conditions ◽  
Trichoderma Atroviride ◽  
Clonostachys Rosea ◽  
Pod Development ◽  
Seed Rot ◽  
Pod Development Stage

Fungal and bacterial antagonists were tested for their inhibition of sporulation of Botrytis cinerea on detached alfalfa florets. Clonostachys rosea, Gliocladium catenulatum, and Trichoderma atroviride were evaluated for protecting young blossoms and pods of alfalfa from infection by B. cinerea in vitro. C. rosea was further tested to control pod rot and seed rot caused by B. cinerea under field conditions. The results showed that four of the tested antagonists, C. rosea, G. catenulatum, T. atroviride, and Trichothecium roseum, could inhibit sporulation by B. cinerea on detached alfalfa florets. Both C. rosea and G. catenulatum were effective in suppression of infection of alfalfa pods by B. cinerea when inoculated on fresh petals of alfalfa at the anthesis stage, and their efficacy was greater than that of Trichoderma atroviride. A significant suppression of B. cinerea by C. rosea and G. catenulatum on pods and seed of alfalfa was observed when they were inoculated on senescent petals at the pod-development stage. Results of a field trial indicated that C. rosea applied to upper parts of alfalfa plants significantly suppressed pod rot and seed rot caused by B. cinerea, and significantly increased seed production of alfalfa in each of 3 years. These studies show that C. rosea has potential as a biocontrol agent for control of alfalfa blossom blight caused by B. cinerea.

Sweeping the flies away: evidence from a fruit fly eradication program

2020 ◽  
Vol 47 (5) ◽  
pp. 1920-1962
Author(s):  
Lina Salazar ◽  
Julian Aramburu ◽  
Marcos Agurto ◽  
Alessandro Maffioli ◽  
Jossie Fahsbender
Keyword(s):  
Pest Management ◽  
Prevention And Control ◽  
Regression Discontinuity ◽  
Fruit Fly ◽  
Fruit Production ◽  
Management Program ◽  
Regression Discontinuity Design ◽  
Short Term ◽  
Pre Treatment ◽  
And Control

Abstract This article evaluates the short-term impacts of a fruit fly integrated pest management program in Peru. Exploiting arbitrary variation in the program’s intervention borders, we use a geographical regression discontinuity design to identify the program’s effects on agricultural outcomes. Pre-treatment balance tests show that producer and farm-level pre-treatment characteristics evolve smoothly at the intervention border. Results indicate that farmers within treated areas improved pest knowledge and are more likely to implement prevention and control practices. Also, they increased fruit production and sales. Our findings are confirmed by placebo tests and are robust to alternative regression discontinuity bandwidths and polynomials.

Growth of micropropagated lowbush blueberry with defined fungi in irradiated peat mix

1992 ◽  
Vol 70 (11) ◽  
pp. 2202-2206 ◽  
Author(s):  
Walter Litten ◽  
John M. Smagula ◽  
Yolande Dalpé
Keyword(s):  
Mycorrhizal Fungi ◽  
Stem Tissue ◽  
Γ Irradiation ◽  
Vaccinium Angustifolium ◽  
Hymenoscyphus Ericae ◽  
Ericoid Mycorrhizae ◽  
Lowbush Blueberry ◽  
Vegetative Multiplication ◽  
Mycorrhizal Inoculation

There is an interest in vegetative multiplication of high-yielding clones of Vaccinium angustifolium Ait. to establish or enhance blueberry production. This study evaluates mycorrhizal inoculation as an aid in such propagation from microcuttings. Shoots of Vaccinium angustifolium (clone 7062) generated in vitro were rooted in a peat–vermiculite–perlite substrate with or without ericoid mycorrhizal fungi fortification by Hymenoscyphus ericae or Scytalidium vaccinii and with or without peat sterilization by γ irradiation. Both in irradiated peat mix inoculated with S. vaccinii and in unirradiated peat mix with H. ericae, microcuttings grew taller and branched more than with the four other treatments. The profusely rooted plantlets available from all treatments of the cuttings put on significantly more total length of stems and branches after 167 days in the greenhouse when growing with either inoculant in unirradiated peat than in the unirradiated peat without inoculation. However, the magnitude of difference might be of borderline importance in commercial nursery operations. A higher level of copper and zinc in stem tissue was observed in stem tissue of plants grown with H. ericae with or without irradiation but not with S. vaccinii. Key words: ericoid mycorrhizae, micropropagation, Hymenoscyphus ericae, Scytalidium vaccinii, Vaccinium angustifolium.

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