Natural deep eutectic systems as alternative nontoxic cryoprotective agents

Cryobiology ◽  
2018 ◽  
Vol 83 ◽  
pp. 15-26 ◽  
Author(s):  
Vânia I.B. Castro ◽  
Rita Craveiro ◽  
Joana M. Silva ◽  
Rui L. Reis ◽  
Alexandre Paiva ◽  
...  
Keyword(s):  
Cryoprotective Agents ◽  
Eutectic Systems

Structures Formed by Cryoprotective Agents Used in Freezc-Etching

1971 ◽  
Vol 29 ◽  
pp. 448-449
Author(s):  
G. G. Cocks ◽  
C. E. Cluthe
Keyword(s):  
Aqueous Solution ◽  
Polyethylene Oxide ◽  
Liquid Nitrogen Temperature ◽  
Ice Crystals ◽  
Etching Technique ◽  
Structural Constituent ◽  
Cryoprotective Agents ◽  
Quick Freezing ◽  
Freeze Etching ◽  
Fractured Surface

The freeze etching technique is potentially useful for examining dilute solutions or suspensions of macromolecular materials. Quick freezing of aqueous solutions in Freon or propane at or near liquid nitrogen temperature produces relatively large ice crystals and these crystals may damage the structures to be examined. Cryoprotective agents may reduce damage to the specimem, hut their use often results in the formation of a different set of specimem artifacts.In a study of the structure of polyethylene oxide gels glycerol and sucrose were used as cryoprotective agents. The experiments reported here show some of the structures which can appear when these cryoprotective agents are used.Figure 1 shows a fractured surface of a frozen 25% aqueous solution of sucrose. The branches of dendritic ice crystals surrounded hy ice-sucrose eutectic can be seen. When this fractured surface is etched the ice in the dendrites sublimes giving the type of structure shown in Figure 2. The ice-sucrose eutectic etches much more slowly. It is the smooth continuous structural constituent surrounding the branches of the dendrites.

Natural deep eutectic systems for improved cryopreservation of cells

Cryobiology ◽  
2020 ◽  
Vol 97 ◽  
pp. 256-257
Author(s):  
Kathlyn Hornberger ◽  
Rui Li ◽  
Allison Hubel
Keyword(s):  
Eutectic Systems

Eutectics: formation, properties, and applications

2021 ◽  
Author(s):  
Dongkun Yu ◽  
Zhimin Xue ◽  
Tiancheng Mu
Keyword(s):  
Deep Eutectic Solvents ◽  
Eutectic Systems

Various eutectic systems including eutectic metals, eutectic salts, and deep eutectic solvents have been proposed and applied in engineering, energy and environmental fields.

Effects of cryoprotective agents and freezing on abdominal ganglia of Aplysia

Cryobiology ◽  
1979 ◽  
Vol 16 (4) ◽  
pp. 390-400
Author(s):  
M. Pasic ◽  
Lj. De Sa Faria
Keyword(s):  
Cryoprotective Agents

Cryopreservation of canine spermatozoa: theoretical prediction of optimal cooling rates in the presence and absence of cryoprotective agents

Cryobiology ◽  
2003 ◽  
Vol 47 (2) ◽  
pp. 109-124 ◽  
Author(s):  
Sreedhar Thirumala ◽  
Maria S Ferrer ◽  
Abdul Al-Jarrah ◽  
Bruce E Eilts ◽  
Dale L Paccamonti ◽  
...  
Keyword(s):  
Theoretical Prediction ◽  
Cooling Rates ◽  
Cryoprotective Agents ◽  
Presence And Absence

Effects of bovine sperm cryopreservation using different freezing techniques and cryoprotective agents on plasma, acrosomal and mitochondrial membranes

Andrologia ◽  
2012 ◽  
Vol 44 ◽  
pp. 154-159 ◽  
Author(s):  
R. A. Forero-Gonzalez ◽  
E. C. C. Celeghini ◽  
C. F. Raphael ◽  
A. F. C. Andrade ◽  
F. F. Bressan ◽  
...  
Keyword(s):  
Sperm Cryopreservation ◽  
Mitochondrial Membranes ◽  
Cryoprotective Agents ◽  
Bovine Sperm
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