Virus infection reduces shoot proliferation of in vitro stock cultures and ability of cryopreserved shoot tips to regenerate into normal shoots in ‘Gala’ apple (Malus × domestica)

Cryobiology ◽  
2018 ◽  
Vol 84 ◽  
pp. 52-58 ◽  
Author(s):  
Min-Rui Wang ◽  
Xin-Yi Hao ◽  
Lei Zhao ◽  
Zhen-Hua Cui ◽  
Gayle M. Volk ◽  
...  
Keyword(s):  
Virus Infection ◽  
Malus Domestica ◽  
Shoot Proliferation ◽  
Shoot Tips

scholarly journals 589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516c-516 ◽  
Author(s):  
Richard K. Kiyomoto ◽  
Mark H. Brand
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Differential Mortality ◽  
Free Medium ◽  
Ex Vitro ◽  
Shoot Morphology ◽  
Normal Shoot ◽  
Tissue Proliferation ◽  
Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

scholarly journals Regeneration of plants from alginate-encapsulated shoots of Rhododendron dalhousiae Hook. F.

2011 ◽  
Vol 3 (1) ◽  
pp. 29-33 ◽  
Author(s):  
K. K. Singh ◽  
Bhusan Gurung
Keyword(s):  
Root Formation ◽  
Solid Medium ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Synthetic Seed ◽  
Root Induction ◽  
Ms Medium ◽  
Direct Sowing

A method has been developed for plant regeneration from alginate-encapsulated nodal segments of Rhododendron dalhousiae. Shoot tips collected from in vitro proliferated shoots were used for synthetic seed production. For encapsulation, nodal segments were mixed with MS medium supplemented with 3% sodium alginate and incubated with calcium chloride (60 mM). The maximum frequency (69%) of conversion of encapsulated shoot tips into plantlets was achieved on MS medium containing 25 μM 2-isopentenyladenine (2iP) along with additive such as, 100 mg L-l polyvinyl pyrrolidone (PVP), 100 mg L -l ascorbic acid, 10 mg L-l citric acid. The presence of 2iP (25 μM) with IAA (0.6 μM) improved re-generation. Amongst the two gelling agents used higher shoot proliferation as well as better growth were observed in cultures grown on Agar in comparison to Phytagel medium. Encapsulated nodal segments stored at 4°C for 25 days also showed successful conversion, followed by development into complete plantlets when returned to regeneration medium. Liquid medium was superior over solid medium for root formation and growth. IBA (1.0 μM) was more effective than other auxins for root induction. Plantlets with developed shoot and roots were hardened off to survive ex vitro conditions and successfully established in greenhouse. Possibility of direct sowing of synthetic seeds in the soil was also examined.

scholarly journals In Vitro Propagation of Viburnum opulus ‘Nanum’

1985 ◽  
Vol 3 (2) ◽  
pp. 41-45
Author(s):  
Virginia Hildebrandt ◽  
Patricia M. Harney
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Indoleacetic Acid ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Viburnum Opulus ◽  
Adenine Sulfate ◽  
Growing Shoot

Explants of actively growing shoot tips from greenhouse-grown plants of Viburnum opulus ‘Nanum’ initiated new shoots on a modified Murashige and Skoog (MS) revised medium plus 0.1 mg/L indoleacetic acid (IAA). These shoots were transferred for proliferation to the same medium, but with 1 mg/L 6-benzylamino purine (BA) replacing IAA and the addition of 2.5 mg/L 2-iso-pentenyladenine (2iP). Both adenine sulfate AdS) and NaH2PO4.H2O inhibited shoot proliferation, while gibberellic acid (GA3) and glycine had no effect. The shoots could be rooted either in the basal medium without cytokinin or in vermiculite under mist.

scholarly journals Comparative Rooting of Shoot Tips of Four Japanese Persimmon Cultivars vs. Shoots Regenerated from Roots Cultured In Vitro

HortScience ◽  
2000 ◽  
Vol 35 (5) ◽  
pp. 940-944 ◽  
Author(s):  
Takuya Tetsumura ◽  
Hisajiro Yukinaga
Keyword(s):  
Histological Study ◽  
Adventitious Shoots ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Diospyros Kaki ◽  
Japanese Persimmon ◽  
Rooting Ability ◽  
Regenerated Plants ◽  
In Vitro Roots

When cultured in vitro, roots of four Japanese persimmon (Diospyros kaki L.) cultivars formed adventitious shoots on MS medium with 10 μm zeatin and 0.01 μm indole-3-acetic acid, although their organogenetic capacities varied. Histological study revealed that the origin of the adventitious shoots was the pericycle. The regenerated shoots grew well on the shoot proliferation medium (MS with 5 μm zeatin). Final rooting percentages of shoots regenerated from roots of three of the four cultivars were greater than those of shoots that originated from shoot tips and that had been subcultured >50 times. Shoots regenerated from `Jiro' roots rooted 10 days earlier, had more roots than those from shoot tips, and maintained higher rooting ability over ten subcultures. Rooted `Hiratanenashi' shoots regenerated from roots survived better after acclimatization than those from shoot tips. No obvious variants were observed either in vitro or in the field. The trees regenerated from roots flowered within 4 years. These findings suggest that partial rather than true rejuvenation was responsible for both the early flowering and the juvenile characteristics, i.e., the enhanced rooting ability, observed in the regenerated plants. Chemical name used: 6-(4-hydroxy-3-methylbut-2-enylamino) purine (zeatin).

scholarly journals Thidiazuron and Benzylamino Purine Stimulation of Apple Shoot Proliferation in Vitro

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 629e-629
Author(s):  
Karim H. Al-Juboory ◽  
Jabar H. Al-Niami
Keyword(s):  
Malus Domestica ◽  
Shoot Proliferation ◽  
Shoot Tip ◽  
Ms Medium ◽  
Proliferation In Vitro ◽  
Shoot Tip Explants ◽  
Apple Shoot ◽  
Stimulation Of

Thidiazuron (TDZ) and benzylamino purine stimulated shoot proliferation on shoot tip explants of wild apple (Malus domestica Borkh) when incorporated in Murashige and Skoog (MS) medium at concentrations of 1.0–10 μm. Shoot numbers obtained with TDZ were greater than the number produced when using BA in the medium but the shoots were shorter than with BA. Increasing TDZ levels increased shoot proliferation with 10 μm. Apple shoots were successfully rooted on MS medium with 2.0 mg·L–1 NAA and then transferred to a mixture of 1 peat: 1 perlite: 1 soil and acclimatized for potting.

scholarly journals In vitro propagation of pointed gourd (Trichosanthes dioica Roxb.) through encapsulated shoot tips

1970 ◽  
Vol 34 (4) ◽  
pp. 555-563 ◽  
Author(s):  
MA Malek
Keyword(s):  
Sodium Alginate ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Alginate Beads ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Artificial Seed ◽  
Pointed Gourd

Plants were regenerated from encapsulated shoot tips of pointed gourd. Shoot tips isolated from multiple shoot cultures of AM-8 and AM-15 cultivars of pointed gourd were encapsulated in sodium alginate beads. For germination and shoot proliferation, encapsulated shoot tips (artificial seed) were cultured in MS basal medium containing different concentrations and combinations of BAP and NAA. Use of MS medium resulted in 90% conversion of encapsulated shoot tips into plantlets. The results exhibited that BAP and combinations of BAP and NAA play an important role in germination of artificial seed being encapsulated by sodium alginate beads. The plantlets were successfully established in earthen pot. Under the present study, limited experimental efforts have been made to establish the protocol for encapsulating the shoot tips for the production of artificial seed and their subsequent regeneration. It is the first report in Bangladesh in developing artificial seed production technique using vegetative tissue of pointed gourd. Key Words: In vitro propagation; pointed gourd; shoot tips. DOI: 10.3329/bjar.v34i4.5832Bangladesh J. Agril. Res. 34(4) : 555-563, December 2009

scholarly journals Regeneration of Ficus glomerata Roxb., using shoot tips and nodal explants

1970 ◽  
Vol 39 (1) ◽  
pp. 47-50 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Ficus Glomerata

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/l BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/l IBA + 0.1 mg/l NAA. The survival rate of regenerated plantlets was 82%. Key words: Ficus glomerata Roxb.; Shoot proliferation; Micropropagation; Acclimatization DOI: 10.3329/bjb.v39i1.5525Bangladesh J. Bot. 39(1): 47-50, 2010 (June)

scholarly journals Shoot Proliferation and Rooting in Vitro of Pulmonaria

HortScience ◽  
1998 ◽  
Vol 33 (2) ◽  
pp. 339-341 ◽  
Author(s):  
Dennis P. Stimart ◽  
John C. Mather ◽  
Kenneth R. Schroeder
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Optimum Level ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Ba Genotype

Expanding shoot tips of Pulmonaria `Roy Davidson' and Pulmonaria saccharata `Margery Fish' were cultured in vitro on a modified Murashige and Skoog medium containing BA to establish proliferating cultures for use in comparing BA concentrations on shoot proliferation and rooting. The optimum level for shoot proliferation was 8.8 μm BA. Greatest rooting was on medium without BA. Genotype and time in culture influenced shoot and root counts. Chemical names used: N6-benzyladenine (BA)

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