scholarly journals 589 PS 132 TISSUE PROLIFERATION IN ELEPIDOTE RHODODENDRONS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 516c-516 ◽  
Author(s):  
Richard K. Kiyomoto ◽  
Mark H. Brand
Keyword(s):  
Shoot Proliferation ◽  
Shoot Tips ◽  
Differential Mortality ◽  
Free Medium ◽  
Ex Vitro ◽  
Shoot Morphology ◽  
Normal Shoot ◽  
Tissue Proliferation ◽  
Ex Vitro Growth

Experiments were conducted on tissue proliferation (TP) development and in vitro and ex vitro growth of tissues from plants with (TP+) and without TP (TP-). In 1993 the increase in TP in one-, two-, and three-yr-old `Holden' and `Besse Howells' was 3%, 52%. and 32% and 10%, 26% and 21%, respectively. No differential mortality was observed. Shoot tip cultures initated from TP+ and TP- `Montego' showed 10-12 mo were required for miniaturiziation and multiplication in TP- shoot tips and 4 mo in TP+ shoot tips. TP- cultures require 10 uM 2-iP for normal shoot proliferation; whereas TP+ cultures had to be transferred to hormone-free medium after 6 mo to maintain normal shoot morphology. Cutting propagation from TP- and TP+ plants older than 5 yr, showed persistence of morphological aberrations associated with TP+ plants.

scholarly journals Cryopreservation and Acclimatization of Lycopersicon esculentum (Mill.) Genotypes

2014 ◽  
Vol 42 (2) ◽  
pp. 466-471 ◽  
Author(s):  
Ana COSTE ◽  
Sergiu VALIMAREANU ◽  
Adela HALMAGYI
Keyword(s):  
Lycopersicon Esculentum ◽  
Liquid Nitrogen ◽  
Growth Conditions ◽  
Alginate Beads ◽  
Shoot Tips ◽  
Ex Vitro ◽  
Fresh Weight Basis ◽  
Ex Vitro Growth ◽  
Laminar Air Flow

Romanian tomato (Lycopersicon esculentum Mill.) cultivars have been cryopreserved by encapsulation-dehydration and successfully acclimatized to ex vitro growth conditions. Shoot tips were excised from in vitro grown plants then precultured for 24 h in various sucrose concentrations, dehydrated up to 6 h in laminar air flow prior to direct immersion in liquid nitrogen   (−196°C) for 24 h. Different parameters have been studied: the effects of osmoprotection and desiccation duration on the regrowth of cryopreserved shoot tips, the effects of various IBA concentrations on rooting and the ex vitro cclimatization of plants recovered from liquid nitrogen. The highest frequency of regrowth (72% cv. ‘Pontica’) was obtained when encapsulated explants were precultured in 0.5 M sucrose and the moisture content (fresh weight basis) of alginate beads was 23%. The highest rooting rates (58% to 77%) for all cultivars were observed for shoots grown on MS medium supplemented with 1.0 mg/l IBA. The rooted plants could be easily acclimatized ex vitro with up to 100% survival.

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals Cytokinin-Facilitated Plant Regeneration of Three Brachystelma Species with Different Conservation Status

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1657
Author(s):  
Nqobile P. Hlophe ◽  
Adeyemi O. Aremu ◽  
Karel Doležal ◽  
Johannes Van Staden ◽  
Jeffrey F. Finnie
Keyword(s):  
In Vitro Propagation ◽  
Slow Growth ◽  
Conservation Status ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Valuable Resource ◽  
Ex Vitro ◽  
Nutritional Values ◽  
Over Exploitation

In Africa and Asia, members of the genus Brachystelma are well-known for their diverse uses, especially their medicinal and nutritional values. However, the use of many Brachystelma species as a valuable resource is generally accompanied by the concern of over-exploitation attributed to their slow growth and general small size. The aim of the current study was to establish efficient micropropagation protocols for three Brachystelma species, namely Brachystelma ngomense (endangered), Brachystelma pulchellum (vulnerable) and Brachystelma pygmaeum (least concern), as a means of ensuring their conservation and survival. This was achieved using nodal segments (~10 mm in length) as the source of explants in the presence of different concentrations of three cytokinins (CK) namely N6-benzyladenine (BA), isopentenyladenine (iP) and meta-topolin riboside (mTR), over a period of 6 weeks. The highest (25 µM) concentration of cytokinin treatments typically resulted in significantly higher shoot proliferation. However, each species differed in its response to specific CK: the optimal concentrations were 25 µM mTR, 25 µM iP and 25 µM BA for Brachystelma ngomense, Brachystelma pulchellum and Brachystelma pygmaeum, respectively. During the in vitro propagation, both Brachystelma ngomense and Brachystelma pygmaeum rooted poorly while regenerated Brachystelma pulchellum generally lacked roots regardless of the CK treatments. Following pulsing (dipping) treatment of in vitro-regenerated shoots with indole-3-butyric acid (IBA), acclimatization of all three Brachystelma species remained extremely limited due to poor rooting ex vitro. To the best of our knowledge, the current protocols provide the first successful report for these Brachystelma species. However, further research remains essential to enhance the efficiency of the devised protocol.

scholarly journals Optimization of Micropropagation Protocol for Goji Berry (Lycium barbarum L.)

2016 ◽  
Vol 73 (2) ◽  
pp. 141 ◽  
Author(s):  
Alexandru Fira ◽  
Nirmal Joshee ◽  
Victoria Cristea ◽  
Manuela Simu ◽  
Monica Harta ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
Optimal Number ◽  
Wheat Starch ◽  
Ms Medium ◽  
Ex Vitro ◽  
Benzyl Adenine ◽  
Lycium Barbarum ◽  
Floating Cell ◽  
The Media

Micropropagation of Lycium barbarum cv. 'Ningxia N1' was achieved. The cultures were by initiated by axenical seed germination. The highest shoot proliferation was obtained on the MS media with 1.33 or 2.22 µM benzyl adenine, gelled with wheat starch as an agar alternative. The treatments with 2.22 µM benzyl adenine ensured proliferation rates superior to the ones with 1.33 μM benzyl adenine, but the latter provided longer and more robust shoots. Use of large microcuttings as an explant onto the multiplication media ensured higher in vitro explant survival, higher number of shoots regeneration and more vigorous plantlets. The microcuttings inserted vertically into the media yielded superior growth and multiplication as compared to the microcuttings placed horizontally. The non-rooted, elongated shoots from the treatment 1.33 μM benzyl adenine were either rooted in vitro on a hormone-free MS medium with starch or used for direct ex vitro rooting and acclimatization. The optimal number of microcuttings/vessel for in vitro rooting was 40 and the rooted plantlets were efficiently acclimatized ex vitro by three methods: float hydroculture in floating cell trays, floating perlite, and in Jiffy7 pellets.

scholarly journals An Efficient Method for In Vitro Shoot-Tip Culture and Sporophyte Production Using Selaginella martensii Spring Sporophyte

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 235 ◽  
Author(s):  
Kyungtae Park ◽  
Bo Kook Jang ◽  
Ha Min Lee ◽  
Ju Sung Cho ◽  
Cheol Hee Lee
Keyword(s):  
Nitrogen Sources ◽  
Culture Conditions ◽  
Shoot Tips ◽  
Shoot Tip ◽  
Soil Conditions ◽  
Ms Medium ◽  
Ex Vitro ◽  
Shoot Tip Culture ◽  
Propagation Methods

Selaginella martensii, an evergreen perennial fern that is native to South America and New Zealand, is named “frosty fern” because of its beautiful white-colored leaves and it is used as an ornamental plant. Efficient propagation methods for this species have not been developed. We aimed to develop an efficient propagation method for S. martensii through in vitro culture. We investigated culture conditions that are suitable for shoot-tip proliferation and growth. The optimum shoot-tip culture conditions were determined while using Murashige and Skoog (MS) medium (quarter, half, full, or double strength) and macronutrients (sucrose and two nitrogen sources) at various concentrations. In MS medium, the shoot tips formed a maximum of 6.77 nodes per explant, and each node formed two new shoot tips (i.e., 26 or 64 shoot tips). When using branching segments containing an angle meristem, the shoot-to-rhizophore formation ratio could be controlled by medium supplementation with plant-growth regulators. Sporophytes that were grown from shoot tips in vitro were acclimated in ex vitro soil conditions and successfully survived in the greenhouse. Numerous shoot tips could be obtained from in vitro-grown sporophytes and be proliferated ex vitro to produce a large number of plants. This method provides a way of shortening the time that is required for producing a large stock of S. martensii planting material.

scholarly journals In vitro plantlet production of the endangered Pinguicula vulgaris

2012 ◽  
Vol 7 (1) ◽  
pp. 48-53 ◽  
Author(s):  
Tomás Grevenstuk ◽  
Anabela Romano
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Normal Development ◽  
Shoot Proliferation ◽  
Carnivorous Plant ◽  
Habitat Requirements ◽  
Ex Vitro ◽  
Plantlet Production

AbstractThis study describes the development of a micropropagation protocol for Pinguicula vulgaris using cultures initiated from in vitro produced seedlings. P. vulgaris is a carnivorous plant with a northern, disjunctly circumpolar distribution and specific habitat requirements, and is hence becoming increasingly rare. Shoot proliferation was significantly influenced by Murashige and Skoog (MS) macronutrient concentration, showing higher proliferation rates in 1/4MS, but was not affected by the addition of 0.1 mg/L 6-benzyladenine (BA) or zeatin (Zea). The best medium for propagating P. vulgaris was plant growth regulator (PGR) free ¼MS. An average of 7.62 new shoots per initial explant could be obtained after 8 weeks of culture, of which over 79% produced roots during proliferation. Moreover, rooting percentages of 100% were obtained for the initial explants in all the tested media, including media without PGRs. The plantlets were successfully acclimatized to ex vitro conditions, exhibiting normal development.

scholarly journals 044 VARIETAL DIFFERENCES IN EARLY EX VITRO GROWTH AND DEVELOPMENT OF MICROPROPAGATED PONTEDERIA CORDATA L.

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 434b-434
Author(s):  
Myrna Stenberg ◽  
Michael E. Kane ◽  
Nancy Philman
Keyword(s):  
Growth Rate ◽  
Growth And Development ◽  
Wetland Plants ◽  
Ex Vitro ◽  
Uptake Capacity ◽  
Early Screening ◽  
Pontederia Cordata ◽  
Plantlet Growth ◽  
Ex Vitro Growth

Micropropagation is a commercially viable and ecologically sound method for producing native herbaceous wetland plants used for wetland revegetation projects. The ability to rapidly screen, select and store germplasm of wetland species genotypes with desirable characteristics of growth rate and habit, nutrient uptake capacity, and/or substrate preference would significantly impact how micropropagated wetland plants are marketed. Early screening of plantlet growth ex vitro may provide an efficient method to select for specific characteristics of growth rate and habit. Five micropropagated lines of Pontederia cordata of differing phenotype were established in vitro from Florida populations. Rooted microcuttings were established ex vitro in a shallow outdoor tank. Growth and development were monitored over a 9 week period. Significant differences in shoot growth and number, leaf area and number, flowering and dry weights were observed between the different Pontederia cordata varieties.

scholarly journals In Vitro Responses of Tissues from Rhododendron Plants With and Without Tissue Proliferation

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 873D-873
Author(s):  
Yiqin Ruan ◽  
Mark H. Brand
Keyword(s):  
Growth Rates ◽  
Woody Plant ◽  
Shoot Tips ◽  
Shoot Cultures ◽  
Relative Growth ◽  
Relative Growth Rates ◽  
Tissue Proliferation ◽  
Long Shoots ◽  
Short Shoots

Rhododendron `Montego' shoot cultures initiated from plants with and without tissue proliferation (TP and NTP) served as explant sources for all studies (Note: in vitro TP shoot cultures produce primarily dwarf shoots, some long shoots, and stem tumors). Calli induced from TP leaves and tumors and NTP leaves were cultured on woody plant (WP) medium containing NAA and 2-iP. During the first 4 weeks of culture, calli from NTP leaves had higher relative growth rates than calli from TP leaves or tumors. However, calli from TP leaves and tumors grew faster than calli from NTP leaves for all subculture periods that followed. Shoot tips (5 mm) were excised from TP dwarf shoots, TP long shoots, and NTP shoots and were cultured on WP medium with or without 15 μM 2-iP. Shoot tips from TP dwarf and long shoots multiplied on medium without 2-iP, averaging 18.4 and 1.7 shoots per shoot tip in 12 weeks, respectively. Shoot tips from NTP shoots only multiplied when maintained on 2-iP-containing medium. When placed on 2-iP-containing medium, both types of TP shoot tips produced clusters of callus-like nodules that gave rise to highly tumorized, short shoots or leafy meristems.

scholarly journals Morphological Development of Lingonberry as Affected by In Vitro and Ex Vitro Propagation Methods and Source Propagule

HortScience ◽  
2005 ◽  
Vol 40 (3) ◽  
pp. 760-763 ◽  
Author(s):  
Samir C. Debnath
Keyword(s):  
Vegetative Growth ◽  
Nutrient Medium ◽  
Growth And Development ◽  
Shoot Proliferation ◽  
Morphological Development ◽  
Stem Cuttings ◽  
Ex Vitro ◽  
Propagation Methods ◽  
In Vitro Shoot Proliferation

The growth and development of lingonberry (Vaccinium vitis-idaea L.) plants propagated either by conventional softwood cuttings or by in vitro shoot proliferation from nodal explants and by shoot regeneration from excised leaves of micropropagated shoots, were studied in cultivars `Regal', `Splendor', and `Erntedank'. Significant differences were observed between the treatments. After 3 years of growth, the in vitro-derived plants produced more stems, leaves, and rhizomes than the conventional cuttings which rarely produced rhizomes. In vitro culture on nutrient medium apparently induces the juvenile branching characteristics that favor rhizome production. This increase in vegetative growth and rhizome yield of in vitro-derived plants over stem cuttings varied among genotypes.

scholarly journals In vitro multiplication protocol for Curcuma mangga : Studies on carbon, cytokinin source and explant size

2021 ◽  
Vol 16 (1) ◽  
pp. 69-76
Author(s):  
A A Waman ◽  
P Bohra ◽  
R Karthika Devi ◽  
J Pixy
Keyword(s):  
Carbon Source ◽  
Large Scale ◽  
Shoot Proliferation ◽  
Ex Vitro Rooting ◽  
Tropical Species ◽  
Scale Production ◽  
Ex Vitro ◽  
In Vitro Multiplication ◽  
Planting Material

Mango ginger (Curcuma mangga Valeton & Zijp.) is an underutilized rhizomatous species that has been valued in tropical Asian countries as a source of vegetable, spice, salad, medicine, and essential oil. This species is hardy and requires less care for obtaining good yields. Rhizomes are the commonly used propagules for the species, which are also the economic part of the crop. Huge quantity of seed rhizomes is required to promote this crop in larger areas. An efficient in vitro multiplication protocol is one of the options to meet the planting material requirement. Effects of carbon source (glucose, fructose and sucrose) and concentration (1 and 3%, w/v), cytokinins (BAP and meta topolin) and concentration (1 mg/L and 2 mg/L), size of explants (one/ two/ three bud) and IBA treatment (0, 250, 500 and 1,000 mg/L) for concurrent ex vitro rooting cum hardening were studied. Results revealed that for facilitating efficient multiplication, the medium should be supplemented with glucose (3%) as a carbon source and meta topolin (1 mg/L) as cytokinin. Two-bud explant should be used for subculture as it promoted superior shoot proliferation. Concurrent ex vitro rooting cum hardening was possible even without auxin treatment. The present protocol could be useful for large-scale production of quality planting material of this underexploited tropical species.

Sign in / Sign up

Export Citation Format

Share Document