scholarly journals Regeneration of Ficus glomerata Roxb., using shoot tips and nodal explants

1970 ◽  
Vol 39 (1) ◽  
pp. 47-50 ◽  
Author(s):  
AKM Sayeed Hassan ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Shoot Proliferation ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Ficus Glomerata

Shoot tips and nodal explants from in vitro growing seedlings of Ficus glomerata Roxb. (Moraceae). showed best shoot induction (88%) on MS medium supplemented with 0.5 mg/l BAP, where maximum number of shoots were produced per culture. In vitro raised shoots rooted well on half strength of MS medium with 2.0 mg/l IBA + 0.1 mg/l NAA. The survival rate of regenerated plantlets was 82%. Key words: Ficus glomerata Roxb.; Shoot proliferation; Micropropagation; Acclimatization DOI: 10.3329/bjb.v39i1.5525Bangladesh J. Bot. 39(1): 47-50, 2010 (June)

scholarly journals In vitro Shoot Proliferation and Plant Regeneration of Phlogacanthus thyrsiflorus Nees. a Rare Medicinal Shrub of Bangladesh

2011 ◽  
Vol 21 (2) ◽  
pp. 135-141 ◽  
Author(s):  
A.K. M. Sayeed Hassan ◽  
Nadira Begum ◽  
Rebeka Sultana ◽  
Rahima Khatun
Keyword(s):  
Survival Rate ◽  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Shoot Induction ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

An efficient protocol was developed for shoot proliferation and plant regeneration of Phlogacanthus thyrsiflorus Nees. (Acanthaceae) - a rare medicinal shrub of Bangladesh, through in vitro culture using shoot tip and nodal explants. Best shoot induction was observed on MS with 1.0 mg/l BAP + 0.5 mg/l NAA, in which 84.2% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half-strength MS with 0.5 mg/l IBA + 0.5 mg/l NAA. For acclimation and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. Key words: Phlogacanthus thyrsiflorus, Shoot proliferation, Plant regeneration   D. O. I. 10.3329/ptcb.v21i2.10236   Plant Tissue Cult. & Biotech. 21(2): 135-141, 2011 (December)

scholarly journals In vitro shoot proliferation and plant regeneration of Plumbago indica L. (Ractochita), a rare medicinal shrub of Bangladesh

2012 ◽  
Vol 47 (2) ◽  
pp. 197-202
Author(s):  
Fowzia Haque ◽  
AKM Sayeed Hassan ◽  
Miskat Ara Akhter Jahan ◽  
Shyamal K Roy
Keyword(s):  
Plant Regeneration ◽  
Room Temperature ◽  
Shoot Proliferation ◽  
Nodal Explants ◽  
Ms Medium ◽  
Plumbago Indica ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
In Vitro Shoot Proliferation

A protocol was established for in vitro shoot proliferation and plant regeneration of a rare medicinal shrub of Bangladesh, Plumbago indica L. (Plumbaginaceae) using shoot tip and nodal explants. Best shoot induction was observed on MS medium supplemented with 0.5 mg/l       BAP, in which 92% of nodal explants responded to produce maximum number (42.8 ± 1.18) of shoots per explants. In vitro raised shoots       rooted on half strength of MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooting culture tubes were kept in normal room temperature for 7 days before transplanting them in pots containing soil where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 82%.   DOI: http://dx.doi.org/10.3329/bjsir.v47i2.11452   Bangladesh J. Sci. Ind. Res. 47(2), 197-202, 2012    

scholarly journals In vitro mass propagation of Plumbago zeylanica L., through direct organogenesis

2012 ◽  
Vol 47 (3) ◽  
pp. 297-302
Author(s):  
AKMS Hassan ◽  
F Haque ◽  
MAA Jahan ◽  
SK Roy
Keyword(s):  
Room Temperature ◽  
Basal Medium ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Plumbago Zeylanica ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was developed for in vitro mass propagation of an important medicinal shrub, Plumbago zeylanica L., (Plumbaginaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 0.5 mg/l BAP, in which 86.4% of nodal explants responded to produce maximum number (12.4 ± 0.66) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 0.5 mg/l IAA. For acclimatization and transplantation, the plantlets in the rooted culture tubes were kept in normal room temperature for 7 days before transplanting in pots where plantlets were reared for three weeks. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v47i3.13063 Bangladesh J. Sci. Ind. Res. 47(3), 297-302 2012

scholarly journals In vitro regeneration for mass propagation in commercial scale of medicinal plant vitex nigundo L.

1970 ◽  
Vol 18 ◽  
pp. 140-145 ◽  
Author(s):  
Md Abu Hena Mostofa Jamal ◽  
ANM Rubaiyath-Bin Rahman ◽  
Dipak Kumar Paul ◽  
Md Rezuanul Islam
Keyword(s):  
Survival Rate ◽  
Medicinal Plant ◽  
Shoot Tips ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Commercial Scale

Context: It is necessary to focus on the importance of adopting micropropagation technique for mass propagation of the plantlets in commercial scale as well as conservation and distribution of germplasm. Objective: The present investigation has been designed with a view to establishing protocol of in vitro regeneration of medicinal plant species i,e., Vitex nigundo L (Verbenaceae). Materials and Methods: Shoot tips and nodal segments were used for multiple shoot induction. All explants were cultured on MS medium supplemented with various plant growth regulators. HgCl2 was used as surface sterilizing agent. For in vitro rooting, individual shoots (3-4 cm) were cut from the proliferated shoot cultures and implanted on half and full strength of MS with different concentrations and combinations of NAA and IAA. The cultures were incubated for 16 h photoperiod at 25 ± 2ºC under a fluorescent light. Visual observation of culture was made every week. Data on shoot induction and proliferation and root induction were recorded after three weeks of inoculation and used for calculation. For each treatment 15 explants were used and all the treatments were repeated thrice. Established plantlets were transplanted in earthen pots under natural conditions and the survival rate was recorded. Results: The most effective surface sterilization treatment has been found 0.1 % HgCl2 for 7 minutes. Highest number of shoot was observed in MS medium supplemented with 3.0 mg/ BAP. It was rooted well in full MS containing 2.0 mg/l IAA. The survival rate was 85 % and propagated plantlets were successfully acclimatized in soil. Conclusion: It was observed that shoot tips are more responsive for micropropagation of Vitex nigundo L . Thus the fruitful utilization of rapid clonal propagation, germplasm conservation and distribution of Vitex nigundo, important medicinal plant of Bangladesh, is possible. Keywords: Vitex nigundo; Medicinal plant; Shoot induction; Micropropagation; Regeneration. DOI: http://dx.doi.org/10.3329/jbs.v18i0.8790 JBS 2010; 18(0): 140-145

scholarly journals In vitro shoot multiplication of the biodiesel plant Jatropha curcas L. through direct organogenesis

2014 ◽  
Vol 49 (1) ◽  
pp. 41-46 ◽  
Author(s):  
MAKMS Hassan ◽  
N Begum ◽  
LS Bari ◽  
MAA Jahan
Keyword(s):  
Jatropha Curcas ◽  
Basal Medium ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Direct Organogenesis ◽  
Shoot Induction ◽  
Jatropha Curcas L ◽  
Half Strength ◽  
Regenerated Plantlets

An efficient protocol was established for in vitro shoot multiplication of the biodiesel plant, Jatropha curcas L. (Euphorbiaceae) through direct organogenesis using shoot tip and nodal explants. Best shoot induction was observed on MS basal medium supplemented with 1.5 mg/l BAP + 0.5 mg/l NAA, in which 86.2% of nodal explants responded to produce maximum number (7.2 ± 0.68) of shoots per culture. In vitro raised shoots rooted on half strength MS medium with 1.0 mg/l IAA. The survival rate of regenerated plantlets was 85%. DOI: http://dx.doi.org/10.3329/bjsir.v49i1.18854 Bangladesh J. Sci. Ind. Res. 49(1), 41-46, 2014

scholarly journals In vitro Propagation of Solanum capsicoides All. – An Important Therapeutic Agent 'Kantakari'

1970 ◽  
Vol 20 (2) ◽  
pp. 179-184
Author(s):  
N.P. Anish ◽  
M.G. Rajesh ◽  
Jiby Elias ◽  
N. Jayan
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Plant Tissue ◽  
In Vitro Propagation ◽  
Therapeutic Agent ◽  
Shoot Tip ◽  
Shoot Induction ◽  
Half Strength ◽  
Shoot Tip Explants

Shoot tip explants from in vitro germinated seedlings of Solanum capsicoides All. inoculated on MS containing 2 mg/l BA produced maximum shoot induction response (26 shoots per explant). Rooting of the microshoots (19.4 roots per explant) was obtained better in half strength of MS supplemented with NAA (0.5 mg/l). Well rooted plantlets were successfully hardened with 80 per cent survival rate.   Key words: Solanum capsicoides, Propagation, Therapeutic agent   D.O.I. 10.3329/ptcb.v20i2.6912   Plant Tissue Cult. & Biotech. 20(2): 179-184, 2010 (December)

scholarly journals IN VITRO REGENERATION AND MASS PROPAGATION OF AZIMA TETRACANTHA [LAM.] FROM THE LEAF EXPLANTS THROUGH CALLUS CULTURE

2017 ◽  
Vol 4 (2) ◽  
pp. 52-56
Author(s):  
Mallika Devi T
Keyword(s):  
Callus Induction ◽  
In Vitro Regeneration ◽  
Leaf Explants ◽  
Shoot Formation ◽  
Ms Medium ◽  
Apical Leaf ◽  
Half Strength ◽  
Regenerated Plantlets ◽  
Azima Tetracantha

In the present study the protocol for callus induction and regeneration in Azima tetracantha has been developed in culture medium. The young apical leaf explants were used for callus induction on MS medium containing BAP and NAA at 1.0 and 0.4mgl-1 respectively showed maximum callus induction (73%). The amount of callus responded for shoot formation (74%) was obtained in the MS medium containing BAP (1.5 mgl-1) and NAA (0.3mgl-1).The elongated shoots were rooted on half strength medium supplemented with IBA (1.5 mgl-1) and Kn (0.4 mgl-1) for shoots rooted. Regenerated plantlets were successfully acclimatized and hardened off inside the culture and then transferred to green house with better survival rate.

IN VITRO RESPONSE BY Terminalia arjuna GENOTYPES DURING MICROPROPAGATION

2021 ◽  
Vol 9 (1) ◽  
pp. 44-50
Author(s):  
Meena Choudhary ◽  
◽  
Ashok Gehlot ◽  
Sarita Arya ◽  
Inder Dev Arya ◽  
...  
Keyword(s):  
Shoot Proliferation ◽  
In Vitro Rooting ◽  
Terminalia Arjuna ◽  
Ms Medium ◽  
Demand And Supply ◽  
Half Strength ◽  
Modified Ms Medium ◽  
In Vitro Response ◽  
Different Doses

Terminalia arjuna is an important tree of the medicinal and sericulture industry, commonly known as Arjun. It’s bark is rich in secondary metabolites makes this plant highly valuable in medicine industry to treat cardiovascular disease. Overexploitation due to high demand in medicine, low seed germination, limitations of the conventional method of propagation push this plant towards being endangered. To conserve germplasm of such tree species and meet the requirement in medicinal industry, some non-conventional propagation method like micropropagation has been developed. The present work highlighted the effect of three genotypes (G-1, G-2, and G-3) on tissue culture of T. arjuna situated at Jodhpur, Rajasthan, India. In vitro shoot proliferation was achieved on a modified MS medium enriched with BAP + additives. Among the tested genotypes, Genotype -1 showed maximum bud break response (100%) followed by G-3 (93.33 %) and G-2 (86.66%). Further multiplication of these shoots on modified MS medium containing BAP + NAA + additives gave 11.38±0.26 (G-1), 9.44±0.21 (G-2) and 10.22±0.32 (G-3) shoots. In vitro rooting was done by pulse treatment with IBA for 10 min prior to transfer on hormone free half strength MS medium containing 0.1% activated charcoal. Maximum in vitro rooting was obtained in G-1 (80%) followed by G-3 (71.11%) and G-2 (68.88%). In the present study, it was observed that optimum growth in all three genotypes required different doses of Plant Growth Regulator. Thus, by identifying and multiplying the best performing genotypes the gap between demand and supply of such medicinal plant can be fulfilled.

scholarly journals Mass Propagation of Feronia limonia L. through Tissue Culture

1970 ◽  
Vol 45 (1) ◽  
pp. 75-78 ◽  
Author(s):  
Shahina Islam ◽  
Mosfequa Zahan ◽  
Shahina Akter ◽  
Tanjina Akhtar Banu ◽  
Ahashan Habib ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Key Words ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Nodal Explants ◽  
Ms Medium ◽  
Ex Vitro ◽  
Mass Propagation

An efficient mass propagation method for Feronia limonia was developed from excised shoot tips and nodal explants of in vitro grown seedlings. Explants were cultured on MS medium with different conc. of NAA, Kn, IAA and BAP singly or in combinations. Highest number of micro shoots and better plant growth were obtained from these two explants on MS medium supplemented with 0.2 mg/l BAP alone. The regenerated shoots were successfully rooted on MS medium supplemented with 0.5 mg/l NAA. The in vitro raised plantlets were successfully established in soil following the formation of roots with 100% survivability under ex vitro condition. Key words: Feronia limonia; Mass propagation; Node; Shoot tips; Multiple shoot DOI: 10.3329/bjsir.v45i1.5186 Bangladesh J. Sci. Ind. Res. 45(1), 75-78, 2010

scholarly journals Micropropagation of Marsdenia brunoniana Wight & Arn. - A rare antidiabetic plant

2010 ◽  
Vol 20 (1) ◽  
pp. 7-12 ◽  
Author(s):  
A. Ugraiah ◽  
S. Karuppusamy ◽  
T. Pullaiah
Keyword(s):  
Survival Rate ◽  
Key Words ◽  
Growth Regulators ◽  
Plant Tissue ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Plant Micropropagation ◽  
Number Of Shoots

Shoot multiplication of M. brunoniana Wight & Arn. was achieved from the nodal explants of mature plants using MS with different concentrations and combina-tions of growth regulators. Maximum explant response and highest number of shoots per explant was obtained on MS medium fortified with 1.0 mg/l BAP. The highest degree of shoot proliferation was found to be 90%. The combination of BAP and Kn was also found to be effective for regeneration. The regenerated shoots were successfully rooted on MS supplemented with 0.5 mg/l NAA, after sequential hardening; survival rate was 90%.  Key words: Marsdenia brunoniana, Medinal plant, Micropropagation, Conservation D. O.I. 10.3329/ptcb.v20i1.5958 Plant Tissue Cult. & Biotech. 20(1): 7-12, 2010 (June)

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