THE ADDITION OF COCONUT WATER AND THIAMINE TOWARDS CHRYSSANTHENUM MICRO CUTTINGS (Dendranthema grandiflora Tzvelev) IN VITRO

Chryssanthenum is ornamental plant with variety of shape and color which are unique and appealing. So that, it is in great demand in the community. The production of Chrissanthenum conventionally hampered by availability and quality of seeds. Thus, it needs research through tissue culture. The aim of this research is to know interaction between coconut water and thiamine and to determine the best coconut water and thiamine concentration toward Chryssanthenum micro cuttings. This research used laboratory experimental method by using completely randomized design with two factor. The 1st factor was coconut water concentration consisted of three level which were 5%, 10% and 15%. The 2nd factor was thiamine concentration consisted of three level which were 1mg/L, 2 mg/L and 3 mg/L. From the two factors, those were found that, there were nine combination of treatments and repeated 3 times. The variety of data was analyzed by using Analysis of Variance (ANOVA) with level of α=5%, and continued by examining Duncan’s Multiple Range Test (DMRT) with level of α=5%. The result indicated that the interaction of coconut water concentration was 5% and thiamine was 1 mg/L on the parameters when growing shoot. There was also interaction on coconut water concentration which was 10 % and thiamine was 1 mg/L on the parameters in the number of shoots. interaction of coconut water combination was 15% and thiamine was 2 mg/L on fresh weight. The addition of 10% coconut water and 1 mg/L thiamine showed the best result on shoot length.

Eriophyoid mites fauna (acari: prostigmata) on pyrus communis l. In the fomin botanical garden

On the territory of the Academician A.V. Fomin Botanical garden three species of mites of the superfamily Eriophyoidea were found in pear orchards. It has been found that Eriophyes pyri Pgst and Epitrimerus pyri Nal. dominate. For the first time, individuals of Epitrimerus marginemtorguens Nal., have been found on pear 12 leaves. Eriophyes pyri is a widespread and dangerous pest of pears in all localities of cultivating this plant species in Ukraine. Epitrimerus pyri is less harmful in pear plantations of the botanical garden. Epitrimerus marginemtorguens appeared mainly in the second half of the growing season. The phenology of four-legged mites has been clarified and the sequence of Eriophyes pyri leaf population on a growing pear shoot has been determined. The period of formation of 7–9 ordinal leaves on the growing shoot is the key moment when the first generation of mites leaves the old galls and colonizes the newly formed leaves. During this period, the phytophagy moves from a hidden to an open way of life and is available for methods and means used in plant protection. A similar moment is also observed during the migration of the second generation to the apical leaves and the third generation – to the buds for wintering, but this process is greatly extended over time and is not so suitable for applying the acaricides.

Nitrogen Levels Regulate Sugar Metabolism and Transport in the Shoot Tips of Crabapple Plants

To comprehensively understand the responses of carbohydrate metabolism and transport to different levels of nitrogen supply in growing shoot tips of crabapple (Malus hupehensis Rehd), enzyme activities and related genes involved in the sugar metabolism pathway were investigated. The nitrogen and chlorophyll content of plants increased with increasing nitrogen supply. High nitrogen application increased the net photosynthesis rate and the growth rate of shoot tips but decreased the synthesis capability of sucrose and sorbitol in mature leaves. However, the shoot tips of plants under high-nitrogen treatment had higher contents of sucrose and sorbitol than did those under low-nitrogen treatment, while the activity of sucrose phosphate synthase and aldose-6-phosphate was increased and the transporters MdSOT and MdSUT were up-regulated. Moreover, the activities of enzymes involved in sucrose and hexose metabolism (including sucrose synthase, fructokinase, and hexokinase) were enhanced in the shoot tips of plants under high-nitrogen conditions, and the expression levels of MdSUSY3 and MdHK1 were significantly up-regulated. These findings indicate that a high nitrogen supply increases the metabolic capacity of assimilatory substances in shoot tips, accelerates the efficiency of sugar utilization and eventually leads to a rapid increase in the growth of shoot tips. Our results highlight that high nitrogen increases the capacity of sugar unloading and metabolic utilization in growing shoot tissues.

Sugar availability suppresses the auxin-induced strigolactone pathway to promote bud outgrowth

SUMMARYApical dominance occurs when the growing shoot tip inhibits the outgrowth of axillary buds. Apically-derived auxin in the nodal stem indirectly inhibits bud outgrowth via cytokinins and strigolactones. Recently, sugar deprivation was found to contribute to this phenomenon.Using rose and pea, we investigated whether sugar availability interacts with auxin in bud outgrowth control, and the role of cytokinins and strigolactones, in vitro and in planta.We show that sucrose antagonizes auxin’s effect on bud outgrowth, in a dose-dependent and coupled manner. Sucrose also suppresses strigolactone-inhibition of outgrowth, and rms3 strigolactone-perception mutant is less affected by reducing sucrose supply; however, sucrose does not interfere with the regulation of cytokinin levels by auxin, and stimulates outgrowth even with optimal cytokinin supply. These observations were assembled into a computational model where sucrose represses bud response to strigolactones, largely independently of cytokinin levels. It quantitatively captures our observed dose-dependent sucrose-hormones effects on bud outgrowth, and allows us to express outgrowth response to various combinations of auxin and sucrose levels as a simple quantitative law.This study places sugars in the bud outgrowth regulatory network, and paves the way for better understanding of branching plasticity in response to environmental and genotypic factors.

INTERAKSI BAP DAN NAA TERHADAP PERTUMBUHAN EKSPLAN ANGGREK VANDA SECARA IN-VITRO

This research aims to obtain the combination of BAP and NAA concentration which give the best effect on explant growth of Vanda orchid in-vitro. The research was conducted at the biotechnology laboratory Faculty of Agriculture Riau Islamic University Pekanbaru from August to December 2013. The completely randomized design with two factorials (BAP and NAA) was used. The observed parameters included percentage of shoot growing, shoot age, shoot height, shoot number and abnormal embryo somatic. Data were analysed using statistics and BNJ Test at the 5% significance level. The result showed that,interactionally,BAP and NAA applications had a significant effect on height plant with the best concentration without BAP and 1.0 ppm NAA. Individually, the application of BAP effected significantly on percentage of shoot growing, shoot growth, shoot height and shoot number with the best concentration without BAP and 0.1 BAP. Furthermore, the application of NAA influenced significantly on percentage of shoot growing, shoot age, shoot height and shoot number with the best concentration of 1.0 ppm NNA

On the growth and form of shoots

Growing plant stems and shoots exhibit a variety of shapes that embody growth in response to various stimuli. Building on experimental observations, we provide a quantitative biophysical theory for these shapes by accounting for the inherent observed passive and active effects: (i) the active controllable growth response of the shoot in response to its orientation relative to gravity, (ii) proprioception, the shoot's growth response to its own observable current shape, and (iii) the passive elastic deflection of the shoot due to its own weight, which determines the current shape of the shoot. Our theory separates the sensed and actuated variables in a growing shoot and results in a morphospace diagram in terms of two dimensionless parameters representing a scaled local active gravitropic sensitivity, and a scaled passive elastic sag. Our computational results allow us to explain the variety of observed transient and steady morphologies with effective positive, negative and even oscillatory gravitropic behaviours, without the need for ad hoc complex spatio-temporal control strategies in terms of these parameters. More broadly, our theory is applicable to the growth of soft, floppy organs where sensing and actuation are dynamically coupled through growth processes via shape.

The acropetal effects of indole-3-acetic acid in isolated shoot segments of Acer pseudoplatanus L. II. Possible regulation by a vectorial fieid of auxin waves

The acropetal effects of auxin on elongation of axillary buds and on modulation of the wave-like pattern of basipetal efflux of natural auxin to agar from <i>Acer pseudoplatanus</i> L. shoots were studied. When synthetic IAA was applied to cut surfaces of one of two branches the elongation growth of buds situated on the opposite branch was retarded, suggesting regulation independent of the direct action of the molecules of the applied IAA. Oscillations in basipetal transport of natural auxin along the stem segments were observed corroborating the results of other authors using different tree species. Apical application of synthetic IAA for 1 hour to the lateral branch caused a phase shift of the wave-like pattern of basipetal efflux of natural auxin, when the stem segment above the treated branch was sectioned. The same effect was observed evoked by the laterally growing branch which is interpreted as an effect of natural auxin produced by the actively growing shoot. These modulations could be propagated acropetally at a rate excluding direct action of auxin molecules at the sites of measurement. The results seem to corroborate the hypothesis suggesting that auxin is involved in acropetal regulation of shoot apex growth through its effect upon modulation of the vectorial field which arises when the auxin-waves translocate in cambium.

Effect of different fertilizers and amendments on the growth of apple and sour cherry rootstocks in an organic nursery

ABSTRACT Two independent trials were established during the years of 2009 and 2010 with M.26 apple rootstocks and Prunus mahaleb L. seedlings in three different locations to evaluate the effect of seven organic fertilizers and amendments on the growth of rootstocks in an organic nursery in the first year of an operation. The products tested were a microbial consortium, two kinds of vermicomposts extracts, two kind of seaweeds extracts, a stillage from yeast production and a solution of titanium. Plants receiving these products were compared to untreated and minerally or organically (manure) fertilized during vegetative season. Plant growth was evaluated by measuring the increase of the root neck diameter and the height of the tallest upward growing shoot. The application of the different organic fertilizers and amendments induced significantly higher growth of both kinds of rootstocks in all locations and for each year in comparison to control and, in the majority of the cases, to the chemical and manure fertilization. However, some products (BF Quality, Micosat, Humus UP, Tytanit and Vinassa) showed a more consistent effect on the growth of tested rootstocks under different soil and climatic conditions. It can be concluded that fertilization of apple and sour cherry rootstock with organic fertilizers and amendments of different origin and composition is effective in producing of high quality plants.