Long-Term Storage In Liquid Nitrogen Modifies Boar Sperm Motility But Is Not Detrimental To Fertility

Cryobiology ◽  
2019 ◽  
Vol 91 ◽  
pp. 176
Author(s):  
Phil Purdy ◽  
Kara Stewart ◽  
Scott Spiller ◽  
Carrie S. Wilson ◽  
Drew Lugar ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Long Term Storage ◽  
Boar Sperm ◽  
Term Storage

Effect of long term storage in LN2 on bacterial load and preservability of semen in Murrah bulls

2015 ◽  
Author(s):  
G. S. Meena ◽  
V. S. Raina ◽  
M. Bhakat ◽  
T. K. Mohanty ◽  
A. K. Gupta ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Incubation Period ◽  
Sperm Motility ◽  
Sperm Quality ◽  
Bacterial Load ◽  
Storage Period ◽  
Least Square ◽  
Long Term Storage ◽  
Term Storage

Successful preservation of semen for longer period can be achieved by maintaining the viability of spermatozoa from its collection till its use for insemination. Therefore, we perceived the idea to determine the effect of long term storage of cryopreserved buffalo semen on sperm quality, bacterial load and fertility. Twenty years semen samples (1981-2000) from twenty bulls and the data on conception rates of these bulls were collected from record room at ABRC, ICAR- NDRI, Karnal. The data was analyzed using least square analysis. The differences in individual motility percent in the semen between estimated initially at the time of freezing and estimates after storage (at the time of evaluation) were 6.49, 14.09, 13.13 and 6.02 percent, respectively. Even after long term storage (up to 20 years) there were little changes in the sperm motility percent. Changes in non-eosinophilic sperm count, sperm abnormalities, HOST and acrosome status, were less up to 20 years storage of semen in liquid nitrogen and the differences were non-significant between different years of storage semen in liquid nitrogen. In similar fashion the microbial load in semen was decreased with the increased storage period of semen in liquid nitrogen, but the differences were non significant. The differences in sperm motility percent between 370C and room temperature were not significant, but the temperature and incubation period significantly (P<0.01) influenced motility percent estimates. Whereas, temperature-incubation period interaction was not found to be significant. The conception rate and sperm motility was highly and positively correlated (r=0.67 and 0.55) with sperm oocyte attachment. It can be concluded that there was little change occurred in semen quality even after twenty years of storage in liquid nitrogen without affecting fertility of semen.

scholarly journals Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Wiesław Bielas ◽  
Wojciech Niżański ◽  
Agnieszka Partyka ◽  
Anna Rząsa ◽  
Ryszard Mordak
Keyword(s):  
Sperm Parameters ◽  
Dna Integrity ◽  
Long Term Storage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Term Storage

Sperm motility evaluation following long-term storage (5 years) of cryopreserved sea bream (Sparus aurata L., 1758) semen

2015 ◽  
Vol 31 ◽  
pp. 104-107 ◽  
Author(s):  
A. Fabbrocini ◽  
R. D'Adamo ◽  
S. Pelosi ◽  
L. F. J. Oliveira ◽  
F. Del Prete ◽  
...  
Keyword(s):  
Sperm Motility ◽  
Sparus Aurata ◽  
Sea Bream ◽  
Long Term Storage ◽  
Term Storage

Somatic polyembryogenesis in embryogenic cell masses of Piceaabies (Norway spruce) and Pinustaeda (loblolly pine) after thawing from liquid nitrogen

1987 ◽  
Vol 17 (9) ◽  
pp. 1130-1134 ◽  
Author(s):  
P. K. Gupta ◽  
D. J. Durzan ◽  
B.J. Finkle
Keyword(s):  
Liquid Nitrogen ◽  
Loblolly Pine ◽  
Somatic Embryos ◽  
Cell Mass ◽  
Embryogenic Cell ◽  
Long Term Storage ◽  
Embryogenic Cell Lines ◽  
Improvement Programs ◽  
Term Storage

We describe a method for the possible cryopreservation of embryogenic callus of Piceaabies and Pinustaeda at −196 °C and the regeneration of somatic embryos from thawed cells of subcultured embryonal–suspensor masses. Piceaabies and Pinustaeda were frozen without cryoprotective agent, in the presence of dimethyl sulfoxide (10%), or in a mixture of polyethylene glycol, glucose, and dimethylsulfoxide (10, 8, and 10% w/v, respectively). Cell masses placed in plastic vials or aluminum envelopes were frozen at 1 °C/min to −30 °C and then immersed for 10 min in liquid nitrogen. Cells were thawed rapidly and placed on modified MS subculture medium. Six to seven somatic embryos per gram of fresh weight were regenerated from each piece of frozen cell mass as compared with 12–13 embryos per gram from unfrozen cells. Post-thaw cell growth was inhibited initially by up to 5 weeks. Inhibition was reversed after the third 10-day subculture. Results suggest that the long-term storage of embryogenic cell lines in liquid nitrogen may be feasible for tree improvement programs in circumstances where testing of progeny may take several years.

scholarly journals Microbial occurrence in liquid nitrogen storage tanks: a challenge for cryobanking?

2021 ◽  
Author(s):  
Felizitas Bajerski ◽  
Manuela Nagel ◽  
Joerg Overmann
Keyword(s):  
Risk Assessment ◽  
Quality Management ◽  
Liquid Nitrogen ◽  
Potential Risk ◽  
Biological Materials ◽  
Cross Contamination ◽  
Storage Tanks ◽  
Long Term Storage ◽  
Term Storage

Abstract Modern biobanks maintain valuable living materials for medical diagnostics, reproduction medicine, and conservation purposes. To guarantee high quality during long-term storage and to avoid metabolic activities, cryostorage is often conducted in the N2 vapour phase or in liquid nitrogen (LN) at temperatures below − 150 °C. One potential risk of cryostorage is microbial cross contamination in the LN storage tanks. The current review summarises data on the occurrence of microorganisms that may compromise the safety and quality of biological materials during long-term storage. We assess the potential for the microbial contamination of LN in storage tanks holding different biological materials based on the detection by culture-based and molecular approaches. The samples themselves, the LN, the human microbiome, and the surrounding environment are possible routes of contamination and can cause cross contaminations via the LN phase. In general, the results showed that LN is typically not the source of major contaminations and only a few studies provided evidence for a risk of microbial cross contamination. So far, culture-based and culture-independent techniques detected only low amounts of microbial cells, indicating that cross contamination may occur at a very low frequency. To further minimise the potential risk of microbial cross contaminations, we recommend reducing the formation of ice crystals in cryotanks that can entrap environmental microorganisms and using sealed or second sample packing. A short survey demonstrated the awareness for microbial contaminations of storage containers among different culture collections. Although most participants consider the risk of cross contaminations in LN storage tanks as low, they prevent potential contaminations by using sealed devices and − 150 °C freezers. It is concluded that the overall risk for cross contaminations in biobanks is relatively low when following standard operating procedures (SOPs). We evaluated the potential sources in detail and summarised our results in a risk assessment spreadsheet which can be used for the quality management of biobanks. Key points • Identification of potential contaminants and their sources in LN storage tanks. • Recommendations to reduce this risk of LN storage tank contamination. • Development of a risk assessment spreadsheet to support quality management.

scholarly journals Development of cryopreservation methods of seed of Nepeta cataria

2021 ◽  
Vol 102 (2) ◽  
pp. 37-42
Author(s):  
Margarita Ishmuratova ◽  
◽  
Damirzhan Baigarayev ◽  
Saltanat Tleukenova ◽  
Elena Gavrilkova ◽  
...  
Keyword(s):  
Liquid Nitrogen ◽  
Room Temperature ◽  
Seed Viability ◽  
Positive Temperature ◽  
Seed Rate ◽  
Nepeta Cataria ◽  
Long Term Storage ◽  
Medical Plant ◽  
Term Storage

This article presents the summarized data on cryopreservation of seeds of the medical plant Nepeta cataria. Cryopreservation is a highly promising method for saving of seed materials, allowing to organize long-term storage without viability loss. The purpose of present work is to optimize conditions of cryopreservation of seed materials of Nepeta cataria. Assessment of seed survival rate in the storage showed a linear decrease in seed viability and energy of germination. After 30 months of storage at the low positive temperature (+5 ºC) in paper pack seed rate decreased to 12.0 % and energy of germination to 11.2 %; after 4 years of storage seeds lost viability. During conduction of research the type of container, condition of thawing, optimal moisture of seeds and cryoprotectants are optimized. The optimal container for cryopreservation in liquid nitrogen was plastic cryo tubes; defrosting at room temperature. The best seed rate is found at moisture 3 %; the best cryoprotectant was glucose, the optimal concentration was 15 %. The result of the research is used for creation of the long-term storage medicinal cultures’ seed bank in the liquid nitrogen.

Sign in / Sign up

Export Citation Format

Share Document