Hypothermic Preservation Of Pancreatic Insulin Producing Β- Cells Using Antifreeze Proteins

SUMMARY Pieces of human foetal pancreas were incubated under control conditions and in media containing different stimuli of insulin release. Insulin secretion was stimulated from the pancreases of foetuses (83–625 g body weight) which were of 16–24 weeks gestational age. Potassium (60 mmol/l), barium (2·54 mmol/l) and ouabain (10−5 mol/l) were effective stimuli in all experiments. Glucagon (5 μg/ml), theophylline (1 mmol/l) and dibutyryl 3′,5′-cyclic adenosine monophosphate (1 mmol/l) stimulated insulin secretion in media containing 0, 0·6 or 3·0 mg glucose/ml. Theophylline and dibutyryl 3′,5′-cyclic adenosine monophosphate were effective in all experients and glucagon stimulated insulin release in four out of six experiments. At all ages studied, histological examination of the pancreas after each experiment revealed islets of Langerhans containing β cells. In most cases the islets were of the mantle type but occasionally bipolar islets were seen. Cellular normality, as judged by light microscopy, was preserved after periods of incubation for up to 5½ h. Glycogen was demonstrable in the pancreatic acinar tissue but not in the islets. The results of these experiments indicate that, between the 16th and 24th week of foetal life, the human β cell is capable of releasing insulin in vitro when stimulated appropriately.

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Extra-pancreatic insulin production in RAt lachrymal gland after streptozotocin-induced islet β-cells destruction

Biochimica et Biophysica Acta (BBA) - General Subjects ◽

10.1016/j.bbagen.2007.05.002 ◽

2007 ◽

Vol 1770 (8) ◽

pp. 1128-1135 ◽

Cited By ~ 9

Author(s):

Daniel Andrade Cunha ◽

Mônica C. de Alves ◽

Luiz Fabrizio Stoppiglia ◽

Angélica Gobbi Jorge ◽

Carolina Maria Módulo ◽

...

Keyword(s):

Insulin Production ◽

Β Cells ◽

Lachrymal Gland ◽

Pancreatic Insulin

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A subcellular model of glucose-stimulated pancreatic insulin secretion

Philosophical Transactions of The Royal Society A Mathematical Physical and Engineering Sciences ◽

10.1098/rsta.2008.0120 ◽

2008 ◽

Vol 366 (1880) ◽

pp. 3525-3543 ◽

Cited By ~ 40

Author(s):

Morten Gram Pedersen ◽

Alberto Corradin ◽

Gianna M Toffolo ◽

Claudio Cobelli

Keyword(s):

Insulin Secretion ◽

Glucose Concentration ◽

Rate Of Change ◽

Β Cells ◽

Biphasic Insulin ◽

Pancreatic Insulin ◽

Biphasic Insulin Secretion ◽

Qualitative Pattern ◽

Glucose Stimulated Insulin Secretion ◽

The Individual

When glucose is raised from a basal to stimulating level, the pancreatic islets respond with a typical biphasic insulin secretion pattern. Moreover, the pancreas is able to recognize the rate of change of the glucose concentration. We present a relatively simple model of insulin secretion from pancreatic β-cells, yet founded on solid physiological grounds and capable of reproducing a series of secretion patterns from perfused pancreases as well as from stimulated islets. The model includes the notion of distinct pools of granules as well as mechanisms such as mobilization, priming, exocytosis and kiss-and-run. Based on experimental data, we suggest that the individual β-cells activate at different glucose concentrations. The model reproduces most of the data it was tested against very well, and can therefore serve as a general model of glucose-stimulated insulin secretion. Simulations predict that the effect of an increased frequency of kiss-and-run exocytotic events is a reduction in insulin secretion without modification of the qualitative pattern. Our model also appears to be the first physiology-based one to reproduce the staircase experiment, which underlies ‘derivative control’, i.e. the pancreatic capacity of measuring the rate of change of the glucose concentration.

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Antidiabetes of Combination of Fractionated-extracts of Andrographispaniculata and Centellaasiatica in Neonatal Streptozotocin-induced Diabetic Rats

INDONESIAN JOURNAL OF PHARMACY ◽

10.22146/ijp.1135 ◽

2020 ◽

pp. 312-322

Author(s):

Reni Ariesta ◽

La Ode Muhammad Fitrawan ◽

Agung Endro Nugroho ◽

Suwidjiyo Pramono

Keyword(s):

Blood Glucose ◽

Diabetic Rats ◽

Β Cells ◽

Β Cell ◽

Antioxidative Effect ◽

Glucose Levels ◽

Blood Glucose Levels ◽

Pancreatic Insulin ◽

Treatment Of Diabetes ◽

Old Rats

Many medicinal plants are widely grown in South- and Southeast Asia countries. Some of them are traditionally used for treatment of diabetes mellitus such as Andrographispaniculata and Centellaasiatica. In the study, we provided fractionated-extracts of A. paniculata and C. asiatica to increase the concentration of their active compounds and eliminate unexpected substances. The aim of the study was to evaluate the antidiabetes effect of the combination of their fractionated-extracts in male neonatal streptozotocin (STZ)-induced diabetic rats. In the study, diabetes was injected intraperitoneally 90mg.kg-1 BWSTZ to two day-old rats. At the age of three months, the rats were administered with the combination of both fractionated-extracts for 14 consecutive days. We evaluated antidiabetes with parameters of blood glucose levels, morphology of pancreatic islet, β-cell density as well as immunohistochemical pancreatic insulin. The levels of MDA, SOD and GPx were also determined about oxidative stress change after treatment with the combination. In the study, the combination succeeded to lower the blood glucose level in neonatal STZ-induced diabetic rats. Inline with fact, improvement of rat pancreatic islets and β cells density, as well as moderate restoration of pancreatic insulin, were observed after treatment with the combination. The substance could decrease the level of MDA, and increase the levels of SOD and GPx. In conclusion, the combination of fractionated-extracts of A. paniculata and C. asiatica exhibited potential antidiabetic effect to its antioxidative effect in male neonatal STZ-induced diabetes rats.

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SPECIFIC MONOCLONAL ANTIBODIES AGAINST THE SURFACE OF RAT ISLET β CELLS

Cell Biology International ◽

10.1016/s1065-6995(02)90952-2 ◽

2002 ◽

Vol 26 (9) ◽

pp. 817-828 ◽

Cited By ~ 4

Author(s):

C Konidaris

Keyword(s):

Monoclonal Antibodies ◽

Β Cells

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β-cells in the lab

Nature India ◽

10.1038/nindia.2009.160 ◽

2009 ◽

Keyword(s):

Β Cells

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Oleate rescues INS-1E β-cells from palmitate-induced ER-stress through a reduced phosphorylation of eIF2α

Diabetologie und Stoffwechsel ◽

10.1055/s-0033-1341762 ◽

2013 ◽

Vol 8 (S 01) ◽

Author(s):

D Sommerweiss ◽

T Gorski ◽

S Laue ◽

S Schuster ◽

A Garten ◽

...

Keyword(s):

Er Stress ◽

Β Cells

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MICROCHEMICAL ASSAYS OF GLUCOSE AND GLUCOSE-6-PHOSPHATE IN MAMMALIAN PANCREATIC β-CELLS

Acta Endocrinologica ◽

10.1530/acta.0.0590479 ◽

1968 ◽

Vol 59 (3) ◽

pp. 479-486 ◽

Cited By ~ 12

Author(s):

Lars-Ake Idahl ◽

Bo Hellman

Keyword(s):

Glucose Level ◽

Exocrine Pancreas ◽

The Other ◽

Wide Concentration Range ◽

Β Cells ◽

Pancreatic Β Cells ◽

Enzymatic Cycling ◽

Glucose Diffusion ◽

Significant Barrier ◽

The Brain

ABSTRACT The combination of enzymatic cycling and fluorometry was used for measuring glucose and glucose-6-phosphate in pancreatic β-cells from obese-hyperglycaemic mice. The glucose level of the β-cells corresponded to that of serum over a wide concentration range. In the exocrine pancreas, on the other hand, a significant barrier to glucose diffusion across the cell membranes was demonstrated. During 5 min of ischaemia, the glucose level remained practically unchanged in the β-cells while it increased in the liver and decreased in the brain. The observation that the pancreatic β-cells are characterized by a relatively low ratio of glucose-6-phosphate to glucose may be attributed to the presence of a specific glucose-6-phosphatase.

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