scholarly journals An oxygen-uptake-rate-based estimator of the specific growth rate in Escherichia coli BL21 strains cultivation processes

2021 ◽  
Author(s):  
Arnas Survyla ◽  
Donatas Levisauskas ◽  
Renaldas Urniezius ◽  
Rimvydas Simutis
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Oxygen Uptake ◽  
Specific Growth Rate ◽  
Uptake Rate ◽  
Specific Growth ◽  
Oxygen Uptake Rate ◽  
Escherichia Coli Bl21

Effect of specific growth rate on the production of a recombinant nuclease by Escherichia coli

2003 ◽  
Vol 14 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Li-Chun Cheng ◽  
Lien-I Hor ◽  
Jau-Yann Wu ◽  
Teh-Liang Chen
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth

Influence of specific growth rate over the secretory expression of recombinant potato carboxypeptidase inhibitor in fed-batch cultures of Escherichia coli

2010 ◽  
Vol 45 (8) ◽  
pp. 1334-1341 ◽  
Author(s):  
Juan-Miguel Puertas ◽  
Jordi Ruiz ◽  
Mónica Rodríguez de la Vega ◽  
Julia Lorenzo ◽  
Glòria Caminal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Fed Batch ◽  
Secretory Expression ◽  
Batch Cultures ◽  
Carboxypeptidase Inhibitor

Evolution of Escherichia coli During Growth in a Constant Environment

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 349-358
Author(s):  
Robert B Helling ◽  
Christopher N Vargas ◽  
Julian Adams
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Continuous Culture ◽  
Specific Growth ◽  
Maximum Specific Growth Rate ◽  
Constant Environment ◽  
Single Clone ◽  
Mixed Populations

ABSTRACT Populations of Escherichia coli, initiated with a single clone and maintained for long periods in glucose-limited continuous culture, developed extensive polymorphisms. In one population, examined after 765 generations, two majority and two minority types were identified. Stable mixed populations were reestablished from the isolated strains. Factors involved in the development of this polymorphism included differences in the maximum specific growth rate and in the transport of glucose, and excretion of metabolites by some clones which were utilized by minority clones.

scholarly journals Poly(3-Hydroxybutyrate) Synthesis by Recombinant Escherichia coli arcA Mutants in Microaerobiosis

2006 ◽  
Vol 72 (4) ◽  
pp. 2614-2620 ◽  
Author(s):  
Pablo I. Nikel ◽  
M. Julia Pettinari ◽  
Miguel A. Galvagno ◽  
Beatriz S. Méndez
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Deletion Mutant ◽  
Specific Growth ◽  
Dry Weight ◽  
Low Oxygen ◽  
Oxygen Conditions ◽  
In The Wild ◽  
Microaerobic Conditions

ABSTRACT We assessed the effects of different arcA mutations on poly(3-hydroxybutyrate) (PHB) synthesis in recombinant Escherichia coli strains carrying the pha synthesis genes from Azotobacter sp. strain FA8. The arcA mutations used were an internal deletion and the arcA2 allele, a leaky mutation for some of the characteristics of the Arc phenotype which confers high respiratory capacity. PHB synthesis was not detected in the wild-type strain in shaken flask cultures under low-oxygen conditions, while ArcA mutants gave rise to polymer accumulation of up to 24% of their cell dry weight. When grown under microaerobic conditions in a bioreactor, the arcA deletion mutant reached a PHB content of 27% ± 2%. Under the same conditions, higher biomass and PHB concentrations were observed for the strain bearing the arcA2 allele, resulting in a PHB content of 35% ± 3%. This strain grew in a simple medium at a specific growth rate of 0.69 ± 0.07 h−1, whereas the deletion mutant needed several nutritional additives and showed a specific growth rate of 0.56 ± 0.06 h−1. The results presented here suggest that arcA mutations could play a role in heterologous PHB synthesis in microaerobiosis.

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