In Vivo Evaluation of the Genotoxic Effects of Gonadotropins on Rat Reticulocytes

Here, butylene adipate-co-terephthalate/polypyrrole with nanohydroxyapatite (PBAT/PPy/nHAp) scaffolds were fabricated and characterized. The electrospinning process was carried out using 12 kV, a needle of 23 G, an infusion pump set at 0.3 mL/h, and 10 cm of distance. Afterwards, nHAp was directly electrodeposited onto PBAT/PPy scaffolds using a classical three-electrode apparatus. For in vivo assays (comet assay, acute and chronic micronucleus), 60 male albino Wistar rats with 4 groups were used in each test (n = 5): PBAT/PPy; PBAT/PPy/nHAp; positive control (cyclophosphamide); and the negative control (distilled water). Peripheral blood samples were collected from the animals to perform the comet test after 4 h (for damage) and 24 h (for repair). In the comet test, it was shown that the scaffolds did not induce damage to the % DNA tail and neither for tail length. After the end of 48 h (for acute micronucleus) and 72 h (for chronic micronucleus), bone marrow was collected from each rat to perform the micronucleus test. All of the produced scaffolds did not present genotoxic effects, providing strong evidence for the biological application of PBAT/PPy/nHAp scaffolds.

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In vivo evaluation of the toxic and genotoxic effects of exposure to cobalt nanoparticles using Drosophila melanogaster

Environmental Science Nano ◽

10.1039/c9en00690g ◽

2020 ◽

Vol 7 (2) ◽

pp. 610-622 ◽

Cited By ~ 3

Author(s):

Mohamed Alaraby ◽

Esref Demir ◽

Josefa Domenech ◽

Antonia Velázquez ◽

Alba Hernández ◽

...

Keyword(s):

Drosophila Melanogaster ◽

Environmental Impact ◽

Cobalt Nanoparticles ◽

Potential Toxicity ◽

Genotoxic Effects ◽

In Vivo Evaluation ◽

Safety Issues

Nanomaterials in general and cobalt nanoparticles (CoNPs) in particular are of great interest not only because of their multiple applications and environmental impact, but also for their potential toxicity and safety issues.

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In vivo evaluation of the toxicity, genotoxicity, histopathological, and anti-inflammatory effects of the purified bioglycerol byproduct in biodiesel industry

Journal of Genetic Engineering and Biotechnology ◽

10.1186/s43141-020-00079-x ◽

2020 ◽

Vol 18 (1) ◽

Author(s):

Wesam Taha Basal ◽

Aliaa Mahmoud Issa ◽

Shehab Eldin Sayed Mohammed ◽

Saher Abd-Elhafeez Mazen

Keyword(s):

Animal Models ◽

Fossil Fuel ◽

Good Alternative ◽

Genotoxic Effects ◽

In Vivo Evaluation ◽

Food Industries ◽

Skin Contact ◽

Anti Inflammatory ◽

Biodiesel Industry

Abstract Background Biodiesel has gained an increased popularity as a good alternative for fossil fuel because of its unusual qualities as a biodegradable, nontoxic, and renewable diesel fuel. Hence, the economic utilization of the accumulated bioglycerol byproduct became critically important for the sustainability of biodiesel industry. The purified bioglycerol might be used as a valuable industrial stock in cosmetic, medical, and food industries. However, if the purified product is going to be used in food, drug, or any industry that involves its ingestion or skin contact by human or animals, the product should be thoroughly tested on animal models. Results The present study investigated the acute toxicity, anti-inflammatory, histopathological, and genotoxic effects of zeolite-purified biogylcerol on different animal models. All the previous tests proved the ability of the purification process to improve the qualities of the crude bioglycerol to a degree comparable to the pharmaceutical grade glycerol. Conclusion In other words, it could be concluded that zeolite-purified bioglycerol can be used in different industries that involves products consumed by human or animals.

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