The effect of moderate wine consumption on cytokine secretion by peripheral blood mononuclear cells: A randomized clinical study in coronary heart disease patients

Aim: We aimed to identify the expression profile and role of circular RNAs (circRNAs) in coronary heart disease (CHD). Materials & methods: We performed sequence analysis of circRNAs in peripheral blood mononuclear cells of 70 CHD patients and 30 controls. Eight selected circRNAs were validated using quantitative real-time polymerase chain reaction (qRT-PCR) in human atherosclerotic coronary arteries. Results: In total, 2283 downregulated and 85 upregulated circRNAs were identified in CHD. Parental genes of top 100 dysregulated-circRNAs are related to metabolism and protein modification, and 12 circRNAs might upregulate their CHD-related parental genes through miRNA sponges. Of the eight circRNAs validated in atherosclerotic coronary arteries by qRT-PCR, six were consistent with sequencing results of peripheral blood mononuclear cells. Conclusion: As potential ceRNAs, dysregulated circRNAs may be involved in CHD pathophysiology.

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ISH NIA PS 02-03 The expression of miR-125a-3p in peripheral blood mononuclear cells of patients with Coronary heart disease

Journal of Hypertension ◽

10.1097/01.hjh.0000500649.69781.e8 ◽

2016 ◽

Vol 34 (Supplement 1) ◽

pp. e278

Author(s):

Hailong Dai ◽

Xiaolong Yin ◽

Juan Xiao ◽

Xuefeng Guang

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Interleukin‑37 is increased in peripheral blood mononuclear cells of coronary heart disease patients and inhibits the inflammatory reaction

Molecular Medicine Reports ◽

10.3892/mmr.2019.10805 ◽

2019 ◽

Author(s):

Huimin Li ◽

Chen Shen ◽

Bingni Chen ◽

Jing Du ◽

Bin Peng ◽

...

Keyword(s):

Coronary Heart Disease ◽

Heart Disease ◽

Peripheral Blood Mononuclear Cells ◽

Inflammatory Reaction ◽

Peripheral Blood ◽

Mononuclear Cells ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

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Effect of Cardiopulmonary Bypass on Annexin A1 Expression in Peripheral Blood Mononuclear Cells of Children with Congenital Heart Disease

Journal of Medical Biochemistry ◽

10.2478/v10011-011-0054-y ◽

2012 ◽

Vol 31 (3) ◽

pp. 193-198

Author(s):

Gengxu Zhou ◽

Xiaoyang Hong ◽

Yuhang Liu ◽

Zhichun Feng

Keyword(s):

Congenital Heart Disease ◽

Heart Disease ◽

Peripheral Blood Mononuclear Cells ◽

Peripheral Blood ◽

Congenital Heart ◽

Mononuclear Cells ◽

Annexin A1 ◽

Differentially Expressed Proteins ◽

Peripheral Blood Mononuclear ◽

Blood Mononuclear Cells

Effect of Cardiopulmonary Bypass on Annexin A1 Expression in Peripheral Blood Mononuclear Cells of Children with Congenital Heart DiseaseThis study aimed to investigate the effect of cardiopulmonary bypass (CPB) on Annexin A1 expression in the peripheral blood mononuclear cells (PBMCs) of children with congenital heart disease (CHD). A total of 30 children receiving CPB for interventricular septal defect were included. Peripheral blood was collected before and after CPB. PBMCs were collected by density gradient centrifugation. Protein extraction was performed by lysis and subjected to 2D-QUANT for protein quantitation. Isoelectric focusing electrophoresis (IEF) was carried out followed by gel image analysis. Protein spots with a difference in expression of >1.5 fold were collected as candidate proteins which were subjected to mass spectrometry for the identification of differentially expressed proteins. Western blot assay was employed to confirm the expressions of target proteins. Peripheral blood collected at two time points was subjected to two-dimensional electrophoresis, and a total of 12 differentially expressed proteins were identified. Of them, 5 proteins had decreased expression before CPB (T0) but their expressions increased after CPB (T1); the remaining 7 proteins had increased expressions before CPB but their expressions reduced after CPB. One of these differentially expressed proteins was Annexin A1. Western blot assay confirmed that Annexin A1 expression began to increase at 0.5 h after CPB, and the increase of Annexin A1 was more obvious after CPB. Our findings primarily indicate the potential mechanism underlying the role of PBMC in inflammatory response following CPB, and provide a target for the prevention and control of post-CPB systemic inflammatory response syndrome (SIRS).

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