Three-dimensional electron microscopic studies of the transitional oligodendrocyte associated with the initial stage of myelination in developing rat hippocampal fimbria

2004 ◽  
Vol 148 (2) ◽  
pp. 207-212 ◽  
Author(s):  
Tokiko Ogawa ◽  
Mitsuru Suzuki ◽  
Koichi Matoh ◽  
Katsuya Sasaki
Development ◽  
1971 ◽  
Vol 25 (2) ◽  
pp. 203-212
Author(s):  
Colin L. Berry

An antiserum with specific reactivity against the contractile proteins of the rat heart has been raised in the rabbit. Fractions of the serum have been shown to enter the isolated rat embryo by electron-microscopic studies with ferritin, where they bind to the myocardium, producing degenerative changes. Both IgG and IgM fractions are toxic, producing foetal death in a large proportion of explanted embryos.


Author(s):  
Lee D. Peachey

Stereology provides a theoretical basis for powerful morphometric methods for the estimation of three-dimensional structural parameters from two-dimensional electron micrographs of cells and tissues. These methods assume at the start that one has a sufficiently large set of micrographs containing valid structural data. The task of obtaining from these micrographs the large quantity of data needed to get statistically valid results has been eased in two general ways. Sampling of data in the micrograph can be done rapidly by point and intersection counting methods. An alternate method, planimetry, obtains all the data in the micrograph, but in general is more time-consuming than point and intersection counting. Some of the relative inefficiency of planimetry is compensated when a digital planimeter is coupled with a computer. Areas and lengths can be computed simultaneously as fast as profiles are traced. Furthermore, rapid and numerically accurate compilation and statistical analysis of the data can be done automatically as the planimetry is done, not as a separate step after the data have been obtained.


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