Comparison of automated repetitive-sequence–based polymerase chain reaction and spa typing versus pulsed-field gel electrophoresis for molecular typing of methicillin-resistant Staphylococcus aureus

2011 ◽  
Vol 69 (1) ◽  
pp. 30-37 ◽  
Author(s):  
Deirdre L. Church ◽  
Barbara L. Chow ◽  
Tracie Lloyd ◽  
Daniel B. Gregson
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Repetitive Sequence ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Spa Typing ◽  
Polymerase Chain

scholarly journals Molecular Typing of Methicillin-sensitive and Methicillin-resistant Staphylococcus aureus: What Epidemiologists need to know from Plasmid Analysis to Whole Genome Sequencing.

2016 ◽  
Vol 6 (1) ◽  
pp. 31-43
Author(s):  
Ibrahim A. Al-Zahrani
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Molecular Typing ◽  
Gel Electrophoresis ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Pulsed Field Gel Electrophoresis ◽  
Whole Genome ◽  
Chain Reaction ◽  
Methicillin Resistant ◽  
Polymerase Chain

Methicillin-resistant Staphylococcus aureus is an important nosocomial pathogen. The early identification of an outbreak, making use of a rapid, precise and simple methicillin-resistant Staphylococcus aureus typing technique, can lead to prompt and effective precautions that avoid further spread of the infection. Pulsed-field gel electrophoresis is considered the gold standard for methicillin-resistant Staphylococcus aureus typing and has been recently supported by multilocus sequence typing. SmaI-multiplex polymerase chain reaction typing is a novel polymerase chain reaction -based technique that combines the principles of pulsed-field gel electrophoresis, with simplicity of polymerase chain reaction and can be used in many routine clinical laboratories. Whole genome sequencing typing is a second generation sequencing technology and may ultimately replace the traditional molecular typing methods. This review aims to survey existing molecular typing techniques for Staphylococcus aureus and to discuss information for each method in order to aid researchers and clinical professionals in the selection and implementation of an optimal technique.

Interinstitutional and Intrainstitutional Transmission of a Strain ofAcinetobacter baumanniiDetected by Molecular Analysis Comparison of Pulsed-Field Gel Electrophoresis and Repetitive Sequence–Based Polymerase Chain Reaction

2006 ◽  
Vol 27 (9) ◽  
pp. 981-983 ◽  
Author(s):  
S. Saeed ◽  
M. G. Fakih ◽  
K. Riederer ◽  
A. R. Shah ◽  
R. Khatib
Keyword(s):  
Polymerase Chain Reaction ◽  
Acute Care ◽  
Repetitive Sequence ◽  
Gel Electrophoresis ◽  
Care Facility ◽  
Pulsed Field Gel Electrophoresis ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Pulsed-field gel electrophoresis and repetitive sequence-based polymerase chain reaction provided comparable strain discrimination with minor discordance in typingAcinetobacter baumanniiclinical isolates from patients at our hospital and affiliated institutions. Typing revealed a cluster strain with intrainstitutional and interinstitutional spread during the study period. A long-term acute care facility may have been the reservoir.

scholarly journals Asymptomatic colonization of Staphylococcus aureus with intermediate resistance to vancomycin harboring vanB resistance gene

2017 ◽  
Vol 10 (5) ◽  
pp. 349 ◽  
Author(s):  
Hala Ibrahim Al Al Daghistani
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Resistance Gene ◽  
Gel Electrophoresis ◽  
Vancomycin Resistance ◽  
Pulsed Field Gel Electrophoresis ◽  
Diffusion Method ◽  
Chain Reaction ◽  
Pulsed Field ◽  
Polymerase Chain

Objective: Vancomycin has been used worldwide due to empirical therapy against methicillin-resistant Staphylococcus aureus infections. As a result a selective pressure that favors the outgrowth of vancomycin intermediate S. aureus clones will be created. This study was carried out to evaluate vancomycin resistance pattern of S. aureus in Jordan.Methods: A total of 1179 samples, including 566 (48%) from human and 613 (52%) from animals were examined for the presence of S. aureus using standard biochemical tests and polymerase chain reaction (PCR) amplification of coa gene. Resistance to antibiotics was determined by the disk diffusion method. Methicillin resistance strains were tested for vancomycin resistance by minimal inhibitory concentration (MIC), E- test, and the results were confirmed by amplification of van genes, and Pulsed-field gel electrophoresis (PFGE).Results: The prevalence of S. aureus among human source was: 19.35%, 14%, and 8.8% for nasal, nail, and skin, respectively, and for animal sources 27.3%, 5.51%, and 15.86% for milk, nasal, and meat, respectively. Four VISA strains (1.87%) were found to colonize human nares, nails, and skin with vancomycin MIC of 4-8 μg/ml. Van B resistance gene was detected and PFGE with SmaI-digested VISA genomic DNA revealed two different pulsotypes.Conclusion: This is believed to be the first report of VISA strains containing vanB gene isolated from a routine carriage survey. Effective screening directed to persons colonized with VISA should therefore be a priority.Keywords: Staphylococcus aureus, Vancomycin intermediate Staphylococcus aureus, Pulsed-field gel electrophoresis, Polymerase chain reaction, VanB genes.

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