Eveline Da Cruz Boa Sorte
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Arleana Bom Parto Ferreira de Almeida
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Felipe Augusto Constantino S. da Cruz
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Naiani Domingos Gasparetto
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Isabela De Godoy
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This study aimed to determine the prevalence of Toxoplasma gondii infection using serological and molecular analyses in dogs from Cuiabá, a municipality of the Brazilian Cerrado, and the associated factors involved in the transmission cycle. A cross-sectional study was conducted with dogs from the rural and urban areas of Cuiabá (Mato Grosso) from February 2010 to January 2011, and antibodies and the DNA of Toxoplasma gondii were evaluated using indirect immunofluorescence (IFA) and polymerase chain reaction (PCR). In the study, a total of 269 dogs were evaluated using IFA considering positive titer threshold of ? 16 and buffy coat PCR. Of the 269 dogs surveyed, 48.7% had anti-Toxoplasma gondii antibodies according to IFA, and 15.6% had the parasite’s DNA. The seroprevalence was 62.4% in the rural districts and 40.4% in the urban areas, and the difference between these areas was significant (p = 0.0007). The most common levels of antibody titers were 51 in 256 (39%) dogs, followed by 1024 in 37 (28.2%) dogs. The infectious agent was associated with breed, age, access to the street and the environment in which the animal lived (p <0.05). The serological and molecular results showed that T. gondii infection is active in the canine population in the rural and urban areas of Cuiabá, with a higher risk in dogs residing in rural areas.
Multicenter comparative assessment of the TIB MolBiol Toxoplasma gondii detection kit and four laboratory-developed PCR assays for molecular diagnosis of toxoplasmosis
