Evaluation of the diagnostic performances of two commercially available assays for the detection of enteric adenovirus antigens

2021 ◽  
pp. 115459
Author(s):  
Floriana Bonura ◽  
Chiara Mascarella ◽  
Chiara Filizzolo ◽  
Celestino Bonura ◽  
Donatella Ferraro ◽  
...  
Keyword(s):  
Enteric Adenovirus

Detection and molecular characterization of enteric adenovirus in treated wastewater in the Brazilian Federal District

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
T. S. C. Quintão ◽  
F. G. Silva ◽  
A. L. Pereira ◽  
W. N. Araújo ◽  
P. M. Oliveira ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Federal District ◽  
Treated Wastewater ◽  
Conventional Pcr ◽  
Rainfall Index ◽  
Enteric Adenovirus ◽  
Enteric Adenoviruses

AbstractHuman enteric viruses, such as enteric adenoviruses (HAdV), are known to be involved with gastrointestinal disorders, especially acute gastroenteritis. Several studies have used HAdV as an indicator of water quality, since they are considered highly stable and widely distributed viruses in water matrices. The aim of this study was to detect and genotype HAdVs in water matrices impacted by discharges of treated effluents from wastewater treatment plants (WWTPs). Wastewater treatment plants from the sanitary system of the Brazilian Federal District were assessed in 2018 and 2019. Samples were collected upstream and downstream from discharge points for each WWTP. Viral concentration based on adsorption-elution and conventional PCR was used for molecular detection, and positive samples were sequenced for phylogenetic analysis. Pluviosity data for the period in which the samples were collected were obtained. Our results demonstrated the presence of HAdVs in 27.2% (61/224) of the samples. The positivity was significantly higher in downstream samples compared to upstream. Moreover, the HAdV positivity was higher in downstream samples collected from receiving water bodies impacted by secondary-level WWTPs in comparison with those impacted by tertiary-level WWTPs. Phylogenetic analysis demonstrated the presence of genotypes 40 and 41, with prevalence of HAdV genotype 41. Despite the predominance of HAdV-41, an increasing frequency of the HAdV-40 was associated with higher pluviosity. In conclusion, this study is the first documentation in the Brazilian Federal District dealing with the prevalence and diversity of HAdVs in several WWTP, along with their correlation with rainfall index.

scholarly journals A non-enteric adenovirus A12 gastroenteritis outbreak in Rio de Janeiro, Brazil

2016 ◽  
Vol 111 (6) ◽  
pp. 403-406 ◽  
Author(s):  
Silvana Augusta Rodrigues Portes ◽  
Eduardo de Mello Volotão ◽  
Monica Simões Rocha ◽  
Maria Cristina Rebelo ◽  
Maria da Penha Trindade Pinheiro Xavier ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Gastroenteritis Outbreak ◽  
Enteric Adenovirus

Prevalence of enteric adenovirus from acute gastroenteritis: A five year study

1987 ◽  
Vol 3 (2) ◽  
pp. 147-150 ◽  
Author(s):  
R. Cevenini ◽  
R. Mazzaracchio ◽  
F. Rumpianesi ◽  
M. Donati ◽  
A. Moroni ◽  
...  
Keyword(s):  
Acute Gastroenteritis ◽  
Enteric Adenovirus

scholarly journals Interferon treatment suppresses enteric adenovirus infection in a model gastrointestinal cell-culture system

2012 ◽  
Vol 93 (3) ◽  
pp. 618-623 ◽  
Author(s):  
Victoria Sherwood ◽  
Elizabeth King ◽  
Sabine Tötemeyer ◽  
Ian Connerton ◽  
Kenneth H. Mellits
Keyword(s):  
Cell Culture ◽  
Culture System ◽  
Adenovirus Type ◽  
Host Cells ◽  
Adenovirus Infection ◽  
Cell Culture System ◽  
Cell Culture Model ◽  
Culture Model ◽  
Enteric Adenovirus ◽  
Transcriptional Induction

Exposure to interferon results in the rapid transcriptional induction of genes, many of which function to create an antiviral environment in potential host cells. For the majority of adenoviruses, replication is unaffected by the actions of interferon. It has previously been shown, using non-gastrointestinal cells, that the species F human adenoviruses are sensitive to the action of interferon. Here, we have developed an enterocyte-like cell-culture model to re-evaluate this question, and determined the effects of interferon on species F adenovirus during infection of gastrointestinal cells. We show that species F adenovirus type 40 is sensitive to the effects of interferon in gastrointestinal-like cells, which may help to explain its fastidious growth in culture.

Enteric Adenovirus Infection in Pediatric Small Bowel Transplant Recipients

2001 ◽  
Vol 4 (2) ◽  
pp. 122-128 ◽  
Author(s):  
Maria Parizhskaya ◽  
Janet Walpusk ◽  
George Mazariegos ◽  
Ronald Jaffe
Keyword(s):  
Small Bowel ◽  
Immune Suppression ◽  
Early Recognition ◽  
Stool Culture ◽  
Adenovirus Infection ◽  
Transplant Recipients ◽  
Small Bowel Transplant ◽  
Enteric Adenovirus ◽  
Transplanted Organs ◽  
Diagnosis Of Rejection

Three of 70 small bowel transplant recipients were diagnosed with adenovirus enteritis. The biopsies were performed for surveillance in one patient at 2.7 years after transplantation and in two symptomatic children 1.5 years and 4.5 months after transplantation. In all three patients the characteristic epithelial changes were not noted by the primary observers. Two biopsies had been called “suggestive of acute rejection” and both patients had been so treated. One biopsy had been diagnosed as “regenerative”. Once the epithelial changes were recognized as being viral, confirmation was possible by stool culture in one patient, immunohistochemistry in two patients, or by lift technique of the H&E sections for electron microscopy. The immune suppression was reduced and none of the patients developed disseminated infection. As in other transplanted organs, such as lung and liver, adenovirus infection may be limited largely to the allograft but can be destructive. Early recognition of the characteristic changes that are illustrated can lead to confirmation of the virus and appropriate reduction of immune suppression. A mistaken diagnosis of rejection and augmentation of immune suppression can lead to viral dissemination and potential fatality.

Preparation and characterization of monoclonal antibodies to enteric adenovirus types 40 and 41

1987 ◽  
Vol 94 (3-4) ◽  
pp. 259-265 ◽  
Author(s):  
J. E. Herrmann ◽  
Dorothy M. Perron-Henry ◽  
Darlene Stobbs-Walro ◽  
N. R. Blacklow
Keyword(s):  
Monoclonal Antibodies ◽  
Enteric Adenovirus

scholarly journals Assessment of the Incidence of Enteric Adenovirus Species and Serotypes in Surface Waters in the Eastern Cape Province of South Africa: Tyume River as a Case Study

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Timothy Sibanda ◽  
Anthony I. Okoh
Keyword(s):  
Surface Waters ◽  
Anthropogenic Activities ◽  
Sampling Site ◽  
Eastern Cape ◽  
Conventional Pcr ◽  
Treated Sewage ◽  
Sampling Points ◽  
Enteric Adenovirus ◽  
Treatment Facilities

TaqMan real-time PCR was used for the detection and quantitation of adenoviruses in Tyume River water samples over a 12-month period. A total of 72 samples were analysed, and 22 samples were positive for adenovirus. Of the positive samples, 18 were collected from downstream sampling points. Among the downstream sampling points, adenovirus detection rate increased with distance downstream, being 28%, 33%, and 39% for Alice, Drayini, and Manqulweni, respectively. The Alice sampling site had the highest concentrations of adenovirus ranging between6.54×103 genome copies/L and8.49×104 genome copies/L. The observed trend could have been expected considering the level of anthropogenic activities in areas along the lower stretch of Tyume River, with the major one being the effluent of treated and semi treated sewage from wastewater treatment facilities. Adenovirus detection was sporadic at most sampling sites. Multiplex conventional PCR was used for the detection of clinically important adenovirus species B, C, and F and their serotypes. Species C and F adenoviruses were detected in 77% and 18% of the samples, respectively. Most adenovirus positive samples were obtained from areas of increased population densities. The presence of adenoviruses may confirm the risk of its transmission to the human population.

scholarly journals Enhancement of Enteric Adenovirus Cultivation by Viral Transactivator Proteins

2010 ◽  
Vol 76 (8) ◽  
pp. 2509-2516 ◽  
Author(s):  
Misoon Kim ◽  
Mi Young Lim ◽  
GwangPyo Ko
Keyword(s):  
Cell Line ◽  
Cytopathic Effect ◽  
Cell Cultivation ◽  
Promoter Regions ◽  
Viral Dna ◽  
Viral Transactivator ◽  
Food Borne ◽  
B Virus ◽  
Enteric Adenovirus ◽  
Enteric Adenoviruses

ABSTRACT Human enteric adenoviruses (HAdVs; serotypes 40 and 41) are important waterborne and food-borne pathogens. However, HAdVs are fastidious, are difficult to cultivate, and do not produce a clear cytopathic effect during cell culture within a reasonable time. Thus, we examined whether the viral transactivator proteins cytomegalovirus (CMV) IE1 and hepatitis B virus (HBV) X promoted the multiplication of HAdVs. Additionally, we constructed a new 293 cell line expressing CMV IE1 protein for cultivation assays. We analyzed the nucleic acid sequences of the promoter regions of both E1A and hexon genes, which are considered to be the most important regions for HAdV replication. Expression of either HBV X or CMV IE1 protein significantly increased the promoter activities of E1A and hexon genes of HAdVs by as much as 14-fold during cell cultivation. The promotion of HAdV expression was confirmed by increased levels of both adenoviral DNA and mRNA expression. Finally, the newly developed 293 cell line expressing CMV IE1 protein showed an increase in viral DNA ranging from 574% to 619% compared with the conventional 293 cell line. These results suggest that the newly constructed cell line could be useful for efficient cultivation and research of fastidious HAdVs.

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