Detection and molecular characterization of enteric adenovirus in treated wastewater in the Brazilian Federal District

2021 ◽  
Vol 3 (7) ◽  
Author(s):  
T. S. C. Quintão ◽  
F. G. Silva ◽  
A. L. Pereira ◽  
W. N. Araújo ◽  
P. M. Oliveira ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Federal District ◽  
Treated Wastewater ◽  
Conventional Pcr ◽  
Rainfall Index ◽  
Enteric Adenovirus ◽  
Enteric Adenoviruses

AbstractHuman enteric viruses, such as enteric adenoviruses (HAdV), are known to be involved with gastrointestinal disorders, especially acute gastroenteritis. Several studies have used HAdV as an indicator of water quality, since they are considered highly stable and widely distributed viruses in water matrices. The aim of this study was to detect and genotype HAdVs in water matrices impacted by discharges of treated effluents from wastewater treatment plants (WWTPs). Wastewater treatment plants from the sanitary system of the Brazilian Federal District were assessed in 2018 and 2019. Samples were collected upstream and downstream from discharge points for each WWTP. Viral concentration based on adsorption-elution and conventional PCR was used for molecular detection, and positive samples were sequenced for phylogenetic analysis. Pluviosity data for the period in which the samples were collected were obtained. Our results demonstrated the presence of HAdVs in 27.2% (61/224) of the samples. The positivity was significantly higher in downstream samples compared to upstream. Moreover, the HAdV positivity was higher in downstream samples collected from receiving water bodies impacted by secondary-level WWTPs in comparison with those impacted by tertiary-level WWTPs. Phylogenetic analysis demonstrated the presence of genotypes 40 and 41, with prevalence of HAdV genotype 41. Despite the predominance of HAdV-41, an increasing frequency of the HAdV-40 was associated with higher pluviosity. In conclusion, this study is the first documentation in the Brazilian Federal District dealing with the prevalence and diversity of HAdVs in several WWTP, along with their correlation with rainfall index.

Prediction of the O2 Conditions in the Selector

1994 ◽  
Vol 29 (7) ◽  
pp. 229-237 ◽  
Author(s):  
J. Kruit ◽  
F. Boley ◽  
L. J. A. M. Jacobs ◽  
T. W. M. Wouda
Keyword(s):  
Wastewater Treatment ◽  
Pilot Plant ◽  
Wastewater Treatment Plants ◽  
Coarse Filter ◽  
Plant Research ◽  
Influent Characterization

Influent characterization and biosorption experiments were carried out with settled influent of seven wastewater treatment plants to study the influence of O2 in the selector in relation to the success of developing good settling properties of the sludge. In previous years working selectors were installed and/or pilot plant research was carried out at these wastewater treatment plants. Characterization of the influent was done with help of standard COD and BOD measurements with help of a coarse filter. The research has elucidated that the presence of O2 in the selector, at initial sludge loadings of 3.5-6.5 kg BOD/kg MLSS.d, is important for producing good settling properties of the sludge when the sum of readily biodegradable COD and rapidly hydrolysable COD is greater than 40%. When the sum of sludge COD and slow hydrolysable COD is greater than 50% an unaerated selector can be used.

scholarly journals Ultrafiltration Process in Disinfection and Advanced Treatment of Tertiary Treated Wastewater

Membranes ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 221
Author(s):  
Rafał Tytus Bray ◽  
Katarzyna Jankowska ◽  
Eliza Kulbat ◽  
Aneta Łuczkiewicz ◽  
Aleksandra Sokołowska
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Microscopic Analysis ◽  
Fecal Coliforms ◽  
Treated Wastewater ◽  
Chemical Parameters ◽  
Virus Particles ◽  
E Coli ◽  
The One ◽  
Physical And Chemical

The paper presents the results of research on the use of ultrafiltration, using membranes of 200 and 400 kDa separation, for disinfection of municipal treated wastewater. The research was conducted on a fractional technical scale using real municipal treated wastewater from two large wastewater treatment plants treating most of the wastewater over the one-million polycentric Gdańsk agglomeration (1.2 million inhabitants). UF 200 kDa and UF 400 kDa processes enabled further improvement of the physical and chemical parameters of treated wastewater. Total phosphorus (to below 0.2 mg/L–UF 200 kDa, 0.13 mg/L–UF 400 kDa) and turbid substances (to below 0.2 mg/L, both membranes) were removed in the highest degree. COD was reduced efficiently (to below 25.6 mgO2/L–UF 200 kDa, 26.8 mgO2/L–UF 400 kDa), while total nitrogen was removed to a small extent (to 7.12 mg/L–UF 200 kDa and 5.7 mg/L–UF 400 kDa. Based on the reduction of indicator bacteria; fecal coliforms including E. coli (FC) and fecal enterococci (FE) it was found that the ultrafiltration is an effective method of disinfection. Not much indicator bacterial were observed in the permeate after processes (UF 200 kDa; FC—5 CFU/L; FE—1 CFU/L and UF 400 kDa; FC—70 CFU/L; FE—10 CFU/L. However, microscopic analysis of prokaryotic cells and virus particles showed their presence after the application of both membrane types; TCN 3.0 × 102 cells/mL–UF 200 kDa, 5.0 × 103 cells/mL–UF 400 kDa, VP 1.0 × 105/mL. The presence of potentially pathogenic, highly infectious virus particles means that ultrafiltration cannot be considered a sufficient disinfection method for treated wastewater diverted for reuse or discharged from high load wastewater treatment plants to recreational areas. For full microbiological safety it would be advisable to apply an additional disinfection method (e.g., ozonation).

The first sequenced Sphaerotilus natans bacteriophage– characterization and potential to control its filamentous bacterium host

2021 ◽  
Vol 97 (4) ◽  
Author(s):  
Rute Ferreira ◽  
Rui Amado ◽  
Jorge Padrão ◽  
Vânia Ferreira ◽  
Nicolina M Dias ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Bacterial Communities ◽  
Potential Application ◽  
Deleterious Effect ◽  
Wastewater Treatment Plants ◽  
Lytic Phage ◽  
Filamentous Bacterium ◽  
Sphaerotilus Natans ◽  
Isolation And Characterization

ABSTRACT Bacteriophages (phages) are ubiquitous entities present in every conceivable habitat as a result of their bacterial parasitism. Their prevalence and impact in the ecology of bacterial communities and their ability to control pathogens make their characterization essential, particularly of new phages, improving knowledge and potential application. The isolation and characterization of a new lytic phage against Sphaerotilus natans strain DSM 6575, named vB_SnaP-R1 (SnaR1), is here described. Besides being the first sequenced genome of a Sphaerotilus natans infecting phage, 99% of its 41507 bp genome lacks homology with any other sequenced phage, revealing its uniqueness and previous lack of knowledge. Moreover, SnaR1 is the first Podoviridae phage described infecting this bacterium. Sphaerotilus natans is an important filamentous bacterium due to its deleterious effect on wastewater treatment plants (WWTP) and thus, phages may play a role as novel biotechnological tools against filamentous overgrowth in WWTP. The lytic spectrum of SnaR1 was restricted to its host strain, infecting only one out of three S. natans strains and infection assays revealed its ability to reduce bacterial loads. Results suggest SnaR1 as the prototype of a new phage genus and demonstrates its potential as a non-chemical alternative to reduce S. natans DSM 6575 cells.

Estimation of Kinetic Parameters of Heterotrophic Biomass under Aerobic Conditions and Characterization of Wastewater for Activated Sludge Modelling

1992 ◽  
Vol 25 (6) ◽  
pp. 125-139 ◽  
Author(s):  
J. Kappeler ◽  
W. Gujer
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Kinetic Parameters ◽  
Specific Growth ◽  
Wastewater Treatment Plants ◽  
Simple Method ◽  
Batch Tests ◽  
Different Types ◽  
Heterotrophic Biomass

To predict the behaviour of biological wastewater treatment plants, the Activated Sludge Model No. 1 is often used. For the application of this model kinetic parameters and wastewater composition must be known. A simple method to estimate kinetic parameters of heterotrophic biomass and COD wastewater fractions is presented. With three different types of batch-tests these parameters and fractions can be determined by measuring oxygen respiration. Our measurements showed that the maximum specific growth rate µmax of heterotrophic biomass depends on temperature, reactor configuration and SRT. In typical wastewater treatment plants of Switzerland the amount of readily biodegradable substrate was generally small (about 9 % of the COD in primary effluent). The same method can also be used to determine kinetic parameters of nitrifying biomass.

Characterization of Sewage Sludge of Wastewater Treatment Plants in Alexandria

2003 ◽  
Vol 33 (3) ◽  
pp. 529-542
Author(s):  
Olfat El-Sebaie ◽  
Ahmed Hussein ◽  
Mohamed Ramadan ◽  
Magda Abd El-Atty ◽  
Helaly Helaly
Keyword(s):  
Wastewater Treatment ◽  
Sewage Sludge ◽  
Wastewater Treatment Plants

Characterization of wastewater and activated sludge from European municipal wastewater treatment plants using the NUR test

1998 ◽  
Vol 38 (1) ◽  
pp. 303-310 ◽  
Author(s):  
V. Naidoo ◽  
V. Urbain ◽  
C. A. Buckley
Keyword(s):  
Wastewater Treatment ◽  
Activated Sludge ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Municipal Wastewater Treatment ◽  
Batch Tests ◽  
Denitrification Kinetics ◽  
Municipal Wastewater Treatment Plants

Denitrification kinetics and wastewater characterization of eight different plants in Europe are discussed. Denitrification batch tests revealed three distinct rates except in the cases of Plaisir, Rostock and Orense where 4 rates were observed. The latter three plants revealed atypical rapid initial rates which were between 7 and 21 mgN/gVSS.h. All denitrification kinetics under non-limiting carbon conditions revealed fast first rates which ranged between 3.0 and 7.3 mgN/gVSS.h. Acetate was used to simulate denitrification kinetics with readily biodegradable COD present. Two subsequent rates were observed. Rates 2 and 3 ranged between 2 and 3 mgN/gVSS.h, and 1 and 2 mgN/gVSS.h, respectively. The RBCOD fraction varied between 10 and 19%, except for one of the plants where the value determined was 7%.

scholarly journals Biological Activity of Wastewater Assessed Using in Vitro Cell-Based Assays

2021 ◽  
Author(s):  
Adamo R. Petosa ◽  
Monica Nowierski ◽  
Viviane Yargeau
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plants ◽  
Estrogenic Activity ◽  
Solid Phase ◽  
Treated Wastewater ◽  
Chemical Analyses ◽  
Untreated Wastewater ◽  
17Β Estradiol ◽  
Receiving Waters

Abstract Bioanalytical tools, namely in vitro bioassays, can be employed in tandem with chemical analyses to assess the efficacy of wastewater treatment and the potential for adverse effects from the discharges of wastewater into receiving waters. In the present study, samples of untreated wastewater (i.e. influent) and treated wastewater (i.e. effluent) were collected from two wastewater treatment plants and a wastewater treatment lagoon serving municipalities in southern Ontario, Canada. In addition, grab samples of surface water were collected downstream of the lagoon discharge. After solid phase extraction (SPE) using ion-exchange columns for basic/neutral and acidic compounds, respectively, the extracts were analyzed for a suite of 16 indicator compounds. The two SPE extracts were combined for analysis of biological responses in four in vitro cell-based bioassays. The concentrations of several indicator compounds, including the estrogens, 17β-estradiol and 17α-ethinylestradiol, were below the limits of detection. However, androstenedione and estrone were detected in several influent samples. The concentrations of these steroid hormones and some of the other indicator compounds declined during treatment but acesulfame K, carbamazepine, trimethoprim and DEET persisted in the effluent. The MTS- CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) indicated that cell viability was not affected by exposure to the extracts. The Qiagen Nuclear Receptors 10-Pathway Reporter Array indicated that several cellular pathways were upregulated, with the greatest upregulation observed with the estrogen receptor (i.e. induction ratios 12 to 47) and the liver X receptor (i.e. induction ratios 10 to 45). The ERα CALUX assay indicated that estrogenic activity was lower in effluents compared to influents, with the greatest estrogenic activity observed for grab samples of influent from the lagoon (i.e. 56-215 ng L-1 17β-estradiol equivalents). Finally, the results of the Nrf2 Luciferase Luminescence Assay indicated a lower oxidative stress in the effluent samples. Overall, the present study demonstrates that chemical analyses are limited in their ability to predict or explain reductions in the toxicity of treated wastewater. There are thus advantages to using a combination of chemical analyses and in vitro bioassays to monitor the treatment efficiency of wastewater treatment plants and to predict the potential impacts of wastewater discharges into receiving waters.

scholarly journals Sewer Mining as a Distributed Intervention for Water-Energy-Materials in the Circular Economy Suitable for Dense Urban Environments: A Real World Demonstration in the City of Athens

Water ◽  
2021 ◽  
Vol 13 (19) ◽  
pp. 2764
Author(s):  
Argyro Plevri ◽  
Klio Monokrousou ◽  
Christos Makropoulos ◽  
Christos Lioumis ◽  
Nikolaos Tazes ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Circular Economy ◽  
Water Reuse ◽  
Wastewater Treatment Plants ◽  
Treatment Plant ◽  
Urban Environments ◽  
Treated Wastewater ◽  
Aquifer Recharge ◽  
Treatment Unit ◽  
Plant Nursery

Water reuse and recycling is gaining momentum as a way to improve the circularity of cities, while recognizing the central role of water within a circular economy (CE) context. However, such interventions often depend on the location of wastewater treatment plants and the treatment technologies installed in their premises, while relying on an expensive piped network to ensure that treated wastewater gets transported from the treatment plant to the point of demand. Thus, the penetration level of treated wastewater as a source of non-potable supply in dense urban environments is limited. This paper focuses on the demonstration of a sewer mining (SM) unit as a source of treated wastewater, as part of a larger and more holistic configuration that examines all three ‘streams’ associated with water in CE: water, energy and materials. The application area is the Athens Plant Nursery, in the (water stressed) city of Athens, Greece. SM technology is in fact a mobile wastewater treatment unit in containers able to extract wastewater from local sewers, treat it directly and reuse at the point of demand even in urban environments with limited space. The unit consists of a membrane bioreactor unit (MBR) and a UV disinfection unit and produces high quality reclaimed water for irrigation and also for aquifer recharge during the winter. Furthermore, a short overview of the integrated nutrient and energy recovery subsystem is presented in order to conceptualise the holistic approach and circularity of the whole configuration. The SM technology demonstrates flexibility, scalability and replicability, which are important characteristics for innovation uptake within the emerging CE context and market.

Biofilm characterization of several wastewater treatment plants with rotating biological contactors in Madrid (Spain)

1998 ◽  
Vol 37 (4-5) ◽  
pp. 215-218 ◽  
Author(s):  
B. Pérez-Uz ◽  
C. Franco ◽  
M. Martín-Cereceda ◽  
L. Arregui ◽  
I. Campos ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Species Composition ◽  
Wastewater Treatment Plants ◽  
Species Level ◽  
Plant Performance ◽  
Ciliate Species ◽  
Level Ii ◽  
Rotating Biological Contactors ◽  
One Year

Ciliate communities were studied in the biofilms of three plants with rotating biological contactors (RBCs) over the period of one year. The aims of this study were: (i) to characterize ciliates to species level; (ii) to determine their abundance and spatial variation through the biological system; and (iii) to relate the spatial segregation and richness of ciliate species to plant performance. A number of species ranging from 33 to 67 were identified at the different plants. The overall best represented groups were peritrichs followed by scuticociliates (Las Matas), cyrtophorids (Boadilla) or hypotrichs (Camarma). Comparison of RBCs and activated sludge communities indicated that even though both were mainly constituted by peritrichs, differences in groups and species composition were observed.

scholarly journals Preparation and characterization of activated carbon from sewage sludge: carbonization step

2004 ◽  
Vol 49 (1) ◽  
pp. 139-146 ◽  
Author(s):  
S. Rio ◽  
C. Faur-Brasquet ◽  
L. Le Coq ◽  
D. Lecomte ◽  
P. Le Cloirec
Keyword(s):  
Wastewater Treatment ◽  
Activated Carbon ◽  
Sewage Sludge ◽  
Thermal Treatment ◽  
Wastewater Treatment Plants ◽  
Carbonization Temperature ◽  
Carbon Production ◽  
By Products ◽  
Characterization Of Activated Carbon

Sewage sludges produced from wastewater treatment plants continue to create environmental problems in terms of volume and method of valorization. Thermal treatment of sewage sludge is considered as an attractive method in reducing sludge volume which at the same time produces reusable by-products. This paper deals with the first step of activated carbon production from sewage sludge, the carbonization step. Experiments are carried out on viscous liquid sludge and limed sludge by varying carbonization temperature and heating rate. The results show that carbonized residue properties are interesting for activated carbon production.

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