Prediction of human hepatic clearance for cytochrome P450 substrates via a new culture method using the collagen vitrigel membrane chamber and PXB-cells

2019 ◽  
Vol 34 (1) ◽  
pp. S23
Author(s):  
Ryuji Watari ◽  
Kazutomi Kusano
2013 ◽  
Vol 3 (1) ◽  
pp. 10 ◽  
Author(s):  
Anne Van Vlaslaer ◽  
Russell J Mortishire-Smith ◽  
Claire Mackie ◽  
Xavier Langlois ◽  
Mark E Schmidt

2013 ◽  
Vol 41 (12) ◽  
pp. 2024-2032 ◽  
Author(s):  
Tom S. Chan ◽  
Hongbin Yu ◽  
Amanda Moore ◽  
Salman R. Khetani ◽  
Donald Tweedie

Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.


2021 ◽  
Author(s):  
Ansgar Bokel ◽  
Michael C. Hutter ◽  
Vlada B. Urlacher

Engineered cytochrome P450 monooxygenase CYP154E1 enables the effective synthesis of the potential antidepressant (2R,6R)-hydroxynorketamine via N-demethylation and regio- and stereoselective hydroxylation of (R)-ketamine.


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