scholarly journals Brightened body coloration in female guppies (Poecilia reticulata) serves as an in vivo biomarker for environmental androgens: The example of 17β-trenbolone

2021 ◽  
Vol 224 ◽  
pp. 112698
Author(s):  
Suqiu Zhang ◽  
Hua Tian ◽  
Yang Sun ◽  
Xuefu Li ◽  
Wei Wang ◽  
...  
Keyword(s):  
Poecilia Reticulata ◽  
Body Coloration

scholarly journals Capsule-Transmitted Gut Symbiotic Bacterium of the Japanese Common Plataspid Stinkbug, Megacopta punctatissima

2002 ◽  
Vol 68 (1) ◽  
pp. 389-396 ◽  
Author(s):  
Takema Fukatsu ◽  
Takahiro Hosokawa
Keyword(s):  
Bacterial Species ◽  
Symbiotic Bacterium ◽  
Egg Mass ◽  
Polymorphism Analysis ◽  
Host Insect ◽  
Fitness Effects ◽  
Body Coloration ◽  
Molecular Phylogenetic ◽  
Microbiological Aspects

ABSTRACT The Japanese common plataspid stinkbug, Megacopta punctatissima, deposits small brown particles, or symbiont capsules, on the underside of the egg mass for the purpose of transmission of symbiotic bacteria to the offspring. We investigated the microbiological aspects of the bacteria contained in the capsule, such as microbial diversity, phylogenetic placement, localization in vivo, and fitness effects on the host insect. Restriction fragment length polymorphism analysis of 16S ribosomal DNA clones revealed that a single bacterial species dominates the microbiota in the capsule. The bacterium was not detected in the eggs but in the capsules, which unequivocally demonstrated that the bacterium is transmitted to the offspring of the insect orally rather than transovarially, through probing of the capsule content. Molecular phylogenetic analysis showed that the bacterium belongs to the γ-subdivision of the Proteobacteria. In adult insects the bacterium was localized in the posterior section of the midgut. Deprivation of the bacterium from the nymphs resulted in retarded development, arrested growth, abnormal body coloration, and other symptoms, suggesting that the bacterium is essential for normal development and growth of the host insect.

scholarly journals FORMULATION AND IN VIVO STUDY OF BACILLUS CLAUSII BASED FUNCTIONAL FEED IN GUPPY (POECILIA RETICULATA).

2017 ◽  
Vol 5 (7) ◽  
pp. 2813-2821
Author(s):  
GlindyaBhagya Lakshmi ◽  
◽  
KeerthiTholoor Rajappan ◽  
KeerthiThalakattil Raghavan. ◽  
◽  
...  
Keyword(s):  
Poecilia Reticulata ◽  
In Vivo Study ◽  
Bacillus Clausii

Death of guppy fish (Poecilia reticulata) leukocytes induced by in vivo exposure to temephos and spinosad

2020 ◽  
pp. 1-11
Author(s):  
K. J. G. Díaz-Resendiz ◽  
A. T. Hermosillo-Escobedo ◽  
G. H. Ventura-Ramón ◽  
G. A. Toledo-Ibarra ◽  
D. A. Girón-Pérez ◽  
...  
Keyword(s):  
Poecilia Reticulata ◽  
Guppy Fish

scholarly journals Comparison of in vitro (fish cell line) and in vivo (fish and crustacean) acute toxicity tests in aquatic toxicology

2021 ◽  
Author(s):  
J Kolarova ◽  
J Velisek ◽  
Z Svobodova
Keyword(s):  
Acute Toxicity ◽  
Cell Line ◽  
Cell Lines ◽  
Poecilia Reticulata ◽  
Cost Effective ◽  
Neutral Red ◽  
Toxicity Tests ◽  
Fish Cell

The use of in vitro (fish cell lines) is a cost-effective, very rapid, and informative tool for toxicological assessments. Using the neutral red (NR) assay, we compared the in vitro acute toxicity (20hEC50) of twenty-six chemical substances on a rainbow trout gonad cell line (RTG-2) with their in vivo acute toxicity to Barbados Millions Poecilia reticulata (48hLC50, OECD 203) and crustacean Daphnia magna (48hEC50, OECD 202). The 20hEC50 values obtained by the NR assay were higher in nearly all the cases when compared to the 48hLC50 in P. reticulata and the 48hEC50 in D. magna, indicating that the sensitivity of the RTG-2 cell line was lower compared to P. reticulata and D. magna. A high (r = 0.89) and significant (P < 0.001) correlation was recorded between the 20hEC50 values of the RTG-2 and the 48hEC50 values of D. magna. The correlation between the 20hEC50 values of the RTG-2 and the 48hLC50 values of P. reticulata was lower (r = 0.65; P < 0.001), but also significant. The authors recommend use of the NR assay on the RTG-2 cell lines as a screening protocol to evaluate the toxicity of xenobiotics in aquatic environments to narrow the spectrum of the concentrations for the fish toxicity test.

scholarly journals Isolation and characterisation of virulent Serratia marcescens associated with a disease outbreak in farmed ornamental fish, Poecilia reticulata in Kerala, India

2017 ◽  
Vol 64 (4) ◽  
Author(s):  
Arathi Dharmaratnam ◽  
Raj Kumar ◽  
V. S. Basheer ◽  
Neeraj Sood ◽  
T. Raja Swaminathan ◽  
...  
Keyword(s):  
Serratia Marcescens ◽  
Poecilia Reticulata ◽  
Multiple Drug Resistance ◽  
Clinical Signs ◽  
Ornamental Fish ◽  
Multiple Drug ◽  
Rrna Gene ◽  
Biochemical Tests

Pathogenic strain of Serratia marcescens (NPSM-1) with multiple drug resistance was isolated from guppy Poecilia reticulata with clinical signs of fin rot and was confirmed by biochemical tests and 16S rRNA gene sequencing. The extra cellular proteins (ECP) of the bacteria exhibited marked cytotoxic activity in vitro on Cyprinus carpio koi fin (CCKF) cell line. The in vivo challenge studies confirmed that the isolate was highly pathogenic to fish when the fishes were injected with1 x 104 CFU/fish and the same bacterium was re-isolated from infected fish, post-challenge. S. marcescens produced large zones of haemolysis on 10% sheep blood agar. The bacteria was found to carry virulence genes; extracellular metalloprotease gene (Pr596) and AHL synthase gene (SpnI). The bacterial isolate was tested to determine sensitivity against 16 antibiotics and was sensitive to only 5 viz., cefixime, chloramphenicol, ciprofloxacin, gentamycin and erythromycin. The study indicates that S. marcescens can cause disease in ornamental fish and the bacterium being a known human pathogen, may also cause infections in humans having direct contact with infected fishes. This is the first report describing S. marcescens as a pathogen of freshwater ornamental fish in India.

Geographical variation in the male intromittent organ of the Trinidadian guppy (Poecilia reticulata)

2000 ◽  
Vol 78 (9) ◽  
pp. 1674-1680 ◽  
Author(s):  
Clint D Kelly ◽  
Jean-Guy J Godin ◽  
Ghada Abdallah
Keyword(s):  
Phenotypic Variation ◽  
Poecilia Reticulata ◽  
Natural Populations ◽  
Fish Predation ◽  
Species Variation ◽  
Body Coloration ◽  
Sexually Selected Traits ◽  
Predation Intensity ◽  
Trinidadian Guppy ◽  
Selected Traits

Sexual selection may favour the evolution of elaborated genital traits in males, particularly when phenotypic variation in such traits results in corresponding variation in reproductive success among males in the population. Compared with insects, very little is known about the natural variation in any male genital trait, and its causes, in vertebrates. Here we report on variation in a male intromittent organ both within and between natural populations of a vertebrate, the Trinidadian guppy (Poecilia reticulata). Male guppies inseminate females using an intromittent organ called the gonopodium. We demonstrate that males from populations that have evolved under high fish-predation intensity have, on average, a relatively longer gonopodium than males originating from populations under low fish-predation intensity. Compared with body coloration, the gonopodium exhibited relatively low phenotypic variation, but nonetheless was within the range of known variation for sexually selected traits. The male gonopodium was positively allometric in general. To our knowledge, this is the first report of within-species variation in an intromittent organ and of a positive allometric relationship between male genitalia and body size in a vertebrate species. Our results suggest that the length of the male intromittent organ in the guppy is under selection, which varies geographically.

scholarly journals Genetic segregation for male body coloration and female mate preference in the guppy

2020 ◽  
Author(s):  
Aya Sato ◽  
Masakado Kawata
Keyword(s):  
Artificial Selection ◽  
Poecilia Reticulata ◽  
Male Body ◽  
Selected Lines ◽  
Female Mate Preference ◽  
Small Areas ◽  
Female Response ◽  
Body Coloration ◽  
Genetic Segregation ◽  
Male Coloration

Abstract Objective The purpose of this study was to segregate the genetic lines responsible for the orange area of coloration in males and the response to orange coloration exhibited by females in the guppy ( Poecilia reticulata ) through artificial selection. This study is part of a project that uses QTL-seq to search for candidate genes involved in male orange coloration and female response to male coloration. We created two lines: high-selected lines of males having large areas of orange spots and of females with high response to male orange coloration; and low-selected lines of males having small areas of orange spots and of females with low response to male orange coloration.Results The male orange area and the female response became significantly different between high- and low-selected lines after three generations of artificial selection. This indicates that the differences in the frequencies of alleles at loci affecting the orange area and the female response between the lines increased over the generations through selection.

scholarly journals Genetic segregation for male body coloration and female mate preference in the guppy

2019 ◽  
Author(s):  
Aya Sato ◽  
Masakado Kawata
Keyword(s):  
Artificial Selection ◽  
Poecilia Reticulata ◽  
Male Body ◽  
Selected Lines ◽  
Female Mate Preference ◽  
Small Areas ◽  
Female Response ◽  
Body Coloration ◽  
Genetic Segregation ◽  
Male Coloration

Abstract Objective The purpose of this study was to segregate the genetic lines responsible for the orange area of coloration in males and the response to orange coloration exhibited by females in the guppy (Poecilia reticulata) through artificial selection. This study is part of a project that uses QTL-seq to search for candidate genes involved in male orange coloration and female response to male coloration. We created two lines: high-selected lines of males having large areas of orange spots and of females with high response to male orange coloration; and low-selected lines of males having small areas of orange spots and of females with low response to male orange coloration.Results The male orange area and the female response became significantly different between high- and low-selected lines after three generations of artificial selection. This indicates that the differences in the frequencies of alleles at loci affecting the orange area and the female response between the lines increased over the generations through selection.

scholarly journals Alk and Ltk ligands are essential for iridophore development in zebrafish mediated by the receptor tyrosine kinase Ltk

2017 ◽  
Vol 114 (45) ◽  
pp. 12027-12032 ◽  
Author(s):  
Elizabeth S. Mo ◽  
Qianni Cheng ◽  
Andrey V. Reshetnyak ◽  
Joseph Schlessinger ◽  
Stefania Nicoli
Keyword(s):  
Tyrosine Kinase ◽  
Receptor Tyrosine Kinase ◽  
Tyrosine Kinases ◽  
Physiological Function ◽  
Orphan Receptor ◽  
Oncogenic Potential ◽  
Anaplastic Lymphoma ◽  
Body Coloration ◽  
Mammalian Genomes

Anaplastic lymphoma kinase (Alk) and leucocyte tyrosine kinase (Ltk) were identified as “orphan” receptor tyrosine kinases (RTKs) with oncogenic potential. Recently ALKAL1 and ALKAL2 (also named “augmentor-β” and “augmentor-α” or “FAM150A” and “FAM150B,” respectively) were discovered as physiological ligands of Alk and Ltk. Here, we employ zebrafish as a model system to explore the physiological function and to characterize in vivo links between Alk and Ltk with their ligands. Unlike the two ligands encoded by mammalian genomes, the zebrafish genome contains three genes: aug-α1, aug-α2, and aug-β. Our experiments demonstrate that these ligands play an important role in zebrafish pigment development. Deficiency in aug-α1, aug-α2, and aug-β results in strong impairment in iridophore patterning of embryonic and adult zebrafish that is phenocopied in zebrafish deficient in Ltk. We show that aug-α1 and aug-α2 are essential for embryonic iridophore development and adult body coloration. In contrast, aug-α2 and aug-β are essential for iridophore formation in the adult eye. Importantly, these processes are entirely mediated by Ltk and not by Alk. These experiments establish a physiological link between augmentor ligands and Ltk and demonstrate that particular augmentors activate Ltk in a tissue-specific context to induce iridophore differentiation from neural crest-derived cells and pigment progenitor cells.

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