Death of guppy fish (Poecilia reticulata) leukocytes induced by in vivo exposure to temephos and spinosad

Poecilia reticulata is a freshwater fish from the northeastern part of South America and spread widely to various countries in Asia and other continents. However, research about P. reticulate is limited even though it is a well-known fish species in Indonesia. The purpose of study was to identify the fish species of P. reticulata through DNA barcoding using the COI gene to determine the phylogenetic relationships among fish populations in East Java, Indonesia. In a present study, there were eight samples of P. reticulata from four different freshwater locations in East Java. Extraction, amplification, and sequencing of DNA samples were conducted to obtain the genetic data and construct a phylogenetic tree based on DNA sequences. The COI gene is the most popular markers to study genetic populations and phylogeography among the animal kingdom. Our phylogenetic reconstruction showed a clear that there were two groups of P. reticulata. The first group was obtain through species from East Java, Sukabumi, West Java (KU692776.1), Dominican Republic, Pandeglang, Banten and Myanmar. The second group was P. reticulata from southern Africa, Brazil, and Sukabumi, West Java (KU692775.1). The result of this study indicate that the guppy fish in East Java identic with P. reticulata from West Java (KU692776.1), which a widely used in classification based on evolutionary relationships. The findings of this study have important implication for the development of advance research about adaptation, phylogeny, and evolution of fish, especially of guppy fish.

Download Full-text

Effect of Different Dietary Protein Levels on Survival Rate and Growth Per-formance of Guppy (Poecilia reticulata)

AL-MUKHTAR JOURNAL OF SCIENCES ◽

10.54172/mjsc.v36i2.42 ◽

2021 ◽

Vol 36 (2) ◽

pp. 175-181

Author(s):

Abdalbast H. Fadel ◽

Awad J. Lamin ◽

Randa Rajab Ali ◽

Karema A. Momen

Keyword(s):

Survival Rate ◽

Dietary Protein ◽

Crude Protein ◽

Growth Response ◽

Poecilia Reticulata ◽

Specific Growth ◽

Protein Levels ◽

Significant Difference ◽

Guppy Fish ◽

Density Rate

This study was carried out to evaluate the growth response of guppy Poecilia reticulata to different dietary protein levels. A total of 72 fry of guppy fish of an initial body weight 0.365± 0.01g were distributed randomly into 60 L aquaria at the density rate of 0.05 g/L. Fish were fed to satiation twice daily for eight weeks with diets containing different dietary protein levels (20%, 30%, or 40%.). The survival rate of guppy was unaffected by increasing dietary protein levels. At the end of the experiment, weight gains (g) ranging from 1.93 to 3.55 were obtained corresponding to 20% and 40% crude protein respectively. The growth performance and feeding efficiencies were significantly improved (P < 0.05) by increasing dietary protein levels. However, no significant difference (P > 0.05) was observed in the specific growth rate of fish that were fed 20% and 30% dietary protein levels. The value of the condition factor was significantly high in fish fed at 40% dietary protein. It can be concluded that a 40% dietary protein level is recommended for guppy fish.

Download Full-text

The Acute Single and Mixture Toxicity of Paraquat Dichloride and Deltamethrin to Guppy (Poecillia reticulata)

10.21203/rs.3.rs-665962/v1 ◽

2021 ◽

Author(s):

Rully Adi Nugroho ◽

Cornelis van Gestel

Keyword(s):

Dose Level ◽

Adult Male ◽

Surface Waters ◽

Poecilia Reticulata ◽

Mixture Toxicity ◽

Concentration Addition ◽

Independent Action ◽

Acute Effects ◽

Reference Models ◽

Guppy Fish

Abstract Although herbicide and insecticide contamination of surface waters normally occurs in the form of mixtures, the toxicity interactions displayed by such mixtures have only rarely been characterized. This study evaluated the acute effects of single pesticides (paraquat dichloride and deltamethrin, tested in their commercial formulations Gramoxone 276 SL and Decis 25EC) and their equitoxic mixtures on the survival of adult male guppy fish (Poecilia reticulata). Mixture toxicity was evaluated against the reference models of Concentration Addition (CA) and Independent Action (IA). In the single treatments, the 72h LC10 and LC50 values were 1.5 and 6.0 mg a.s. L-1 and 0.53 and 3.6 µg a.s. L-1 for paraquat dichloride and deltamethrin, respectively. The equitoxic mixtures showed significant paraquat dichloride-deltamethrin antagonism, both based on the CA and the IA model, without significant dose-level dependent deviations.

Download Full-text

Pengaruh lama perendaman induk ikan guppy (Poecilia reticulate) dalam madu terhadap nisbah kelamin jantan (sex reversal) ikan guppy

Acta Aquatica Aquatic Sciences Journal ◽

10.29103/aa.v3i2.327 ◽

2016 ◽

Vol 3 (2) ◽

pp. 75

Author(s):

Nurlina Nurlina ◽

Zulfikar Zulfikar

Keyword(s):

Poecilia Reticulata ◽

Quality Parameters ◽

Soaking Time ◽

Male Ratio ◽

Randomized Design ◽

Male Sex ◽

Completely Randomized Design ◽

Higher Doses ◽

Brood Fish ◽

Guppy Fish

Penelitian ini bertujuan untuk mendapatkan waktu perendaman yang terbaik bagi induk ikan guppy (Poecilia reticulata. Penelitian ini dilaksanakan pada bulan November sampai dengan Januari 2015 yang bertempat di Laboratorium Hatchery dan Teknologi Budidaya Fakultas Pertanian, Universitas Malikussaleh. Menggunakan metode eksperimental dan Rancangan penelitian yang digunakan Rancangan Acak Lengkap (RAL) non faktorial dengan 4 perlakuan dan 3 ulangan. Hasil penelitian menunjukkan bahwa Pengaruh lama perendaman yang Berbeda menunjukkan hasil yang berbeda nyata terhadap keberhasilan jenis kelamin jantan ikan guppy. Dengan hasil perlakuan yang terbaik terdapat pada perlakuan C dengan perendaman 15 jam dengan jumlah rata-rata 89.93%. Sedangkan yang terendah terdapat pada perlakuan (A) perendaman 9 jam yaitu (72.32%). Saran Perlu adanya penelitian lanjutan mengenai jantanisasi dengan menggunakan madu dilakukan dengan dosis yang lebih tinggi dan waktu pemaparan singkat, serta perlu di lakukan penelitian mengenai kemampuan ikan guppy untuk menyerap madu yang di berikan.This study aimed to get the best immersion time for the guppy brood fish (Poecilia reticulate). The research was conducted in November 2014 until January 2015 which was held at the Laboratory of Aquaculture Hatchery and Technology, Faculty of Agriculture, University of Malikussaleh. The method of this study wos experimental menthod and analysis used completely randomized design (CRD) with 4 treatments and 3 replications. The results showed that the soaking time influenced significantly to the success of the male sex of guppy fish. The best treatment of soaking time was a 15- hours immersion with the male ratio 89.93%. While the lowest one was obtaimed in treatment of 9 hours immersion which was of (72.32%) of male. Range Value of water quality parameters during the study, namely the temperature was 26.4 - 28. 0 ° C, pH between 6.5 -8.0. There fore Suggestions for further research by using is to implement the higher doses and shorter exposure time, as well as further rescarch is also needed to do on to ability of guppy fish in absor bigy honey.

Download Full-text

Penjantanan ikan gapi, Poecilia reticulata Peters, 1859 dengan pemberian ekstrak jeroan teripang pasir (Holothuria scabra)

Jurnal Iktiologi Indonesia ◽

10.32491/jii.v18i2.427 ◽

2019 ◽

Vol 18 (2) ◽

pp. 127

Author(s):

Anugerah Saputra ◽

Astri Wulandari ◽

. Ernawati ◽

Muhammad Amri Yusuf ◽

Irvan Eriswandy ◽

...

Keyword(s):

Sea Cucumber ◽

Poecilia Reticulata ◽

Hormone Treatment ◽

Positive Control ◽

Male Fish ◽

Randomized Design ◽

Parent Stock ◽

Completely Randomized Design ◽

Guppy Fish ◽

Different Doses

Guppy fish Poecilia reticulata is one of the most popular freshwater ornamental fish species because it is easy to main-tain, and has beautiful variations of color, especially males. One method to produce male guppy is masculinization me-thod. This study aimed to determine the dose of hormone treatment from visceral organ of sea cucumber to the process masculinization of fish guppy. This study used a completely randomized design with the immersion treatment of parent guppy which has been bred into the extract solution of sea cucumber innards for 24 hours with different doses i.e. 0 mg L-1, 10 mg L-1, 20 mg L-1, 30 mg L-1 and 22 mg L-1 (metiltestosterone as positive control). The parameter measured was the percentage of male fish produced. The results showed that although the effect of the treatment was not significantly different, but it can be said to be significantly different to the treatment of 0 mg L-1 (control) to obtain the best result for masculinization of guppy at 30 mg L-1 immersion and the best survival rate on treatment of 0 mg L-1 (control). The re-sults of this study suggest that the masculinization technology on the parent stock can be done using extracts of sea cu-cumber innards which has been considered as waste.AbstrakIkan gapi Poecilia reticulata merupakan salah satu jenis ikan hias air tawar yang banyak digemari karena mudah dipe-lihara, dan memiliki variasi wama yang indah, terutama jantannya. Salah satu metode untuk menghasilkan ikan gapi jantan adalah dengan metode pembalikan kelamin melalui produksi penjantanan ikan gapi. Penelitian ini bertujuan un-tuk menentukan dosis hormon perlakuan ekstrak jeroan teripang pasir terhadap proses penjantanan ikan gapi. Penelitian ini menggunakan rancangan acak lengkap dengan perlakuan perendaman induk ikan gapi yang telah dipijahkan ke dalam larutan ekstrak jeroan teripang pasir selama 24 jam dengan dosis berbeda, yaitu 0 mg L-1, 10 mg L-1, 20 mg L-1, 30 mg L-1 dan 22 mg L-1 (metiltestosteron sebagai kontrol positif). Parameter yang diukur adalah persentase ikan jantan yang dihasilkan. Hasil penelitian menunjukkan bahwa walaupun pengaruh antarperlakuan yang diberikan tidak berbeda nyata, tetapi dapat dikatakan berbeda nyata terhadap perlakuan 0 mg L-1 (kontrol) sehingga diperoleh hasil terbaik untuk penjantanan ikan gapi pada dosis perendaman 30 mg L-1 dan sintasan terbaik pada perlakuan 0 mg L-1 (kontrol). Hasil penelitian ini memberikan informasi bahwa teknologi penjantanan pada induk ikan gapi dapat dilakukan dengan me-manfaatkan ekstrak jeroan teripang pasir yang selama ini dianggap sebagai limbah.

Download Full-text

Breeding biology of guppy fish, Poecilia reticulata (Peters, 1859) in the laboratory

Journal of the Asiatic Society of Bangladesh Science ◽

10.3329/jasbs.v39i2.17866 ◽

2014 ◽

Vol 39 (2) ◽

pp. 259-267 ◽

Cited By ~ 4

Author(s):

Reza Md Shahjahan ◽

Md Jubayer Ahmed ◽

Rowshan Ara Begum ◽

Md Abdur Rashid

Keyword(s):

Sexual Maturity ◽

Poecilia Reticulata ◽

Developmental Stages ◽

Breeding Biology ◽

Zoological Garden ◽

Peak Period ◽

Optic Cup ◽

The Mean ◽

Egg Diameter ◽

Guppy Fish

The breeding biology of guppy fish, Poecilia reticulata (Cyprinodontiformes: Poiciliidae) was studied during March 2008 to May 2009 in Zoological garden laboratory, Curzon Hall campus, Dhaka University. Guppy bred all over the year except in the winter months December and January with a peak period in July. They were viviparous and multiple breeders, i.e., give birth to fry several times in the breeding season. The mean egg diameter was measured to be (1.02±0.08mm) and fecundity was estimated (40-89) per gram of body weight. The gestation period ranged 25-35 days with an average of 28.1±2.12 days. Developmental stages observed under a compound microscope were classified based on the changes in the developing eye, such as optic cup, early-eyed, middle-eyed, late-eyed, very late-eyed etc. It was noticed that tail portion comes out first at birth. The number of fry per brood ranged from 12 to 60. New born fries were observed with transparent or blackish in colour having slender body with jaws developed on mouth and were fully capable of swimming, eating, and avoiding danger. Guppy grew rapidly, attained sexual maturity at 8 -10 weeks and reached full size in 6 months. DOI: http://dx.doi.org/10.3329/jasbs.v39i2.17866 J. Asiat. Soc. Bangladesh, Sci. 39(2): 259-267, December 2013

Download Full-text

First study of the guppy fish (Poecilia reticulata Peters, ) occurring in natural thermal waters of Serbia

Journal of Applied Ichthyology ◽

10.1111/jai.12218 ◽

2013 ◽

Vol 30 (1) ◽

pp. 160-163 ◽

Cited By ~ 6

Author(s):

M. Milenković ◽

V. Žikić ◽

S. S. Stanković ◽

S. Marić

Keyword(s):

Poecilia Reticulata ◽

Thermal Waters ◽

Guppy Fish

Download Full-text

Maskulinisasi Ikan Guppy (Poecilia Reticulata) Melalui Penggunaan Air Kelapa (Cocos Nucifera) Dengan Konsentrasi Berbeda

Jurnal Akuakultur Rawa Indonesia ◽

10.36706/jari.v7i1.9017 ◽

2019 ◽

Vol 7 (1) ◽

pp. 13-24

Author(s):

Tomi Malik ◽

Mochamad Syaifudin ◽

Mohamad Amin

Keyword(s):

Water Quality ◽

Survival Rate ◽

Poecilia Reticulata ◽

Cocos Nucifera ◽

Sex Reversal ◽

Coconut Water ◽

Randomized Design ◽

Completely Randomized Design ◽

Guppy Fish

Masculinization is one method of sex reversal technique that can direct the sex of fish. The aim of this research is to know the effect of different concentration ofcoconut waterto percentage of male guppy fish through immersion method.This research was conducted in August - October 2018 at Fisheries Basic Laboratory, Program Study of Aquaculture, Sriwijaya University. This research used completely randomized design (CRD) with four treatments and three replications i.e without coconut water (P0), coconut water 20%(P1), coconut water 30% (P2) and coconut water 40% (P3). The observed parameters were the percentage of male guppy, survival rate and water quality (temperature, pH, ammonia and dissolved oxigen). The result showed the percentage of male guppy was 46.66 % (P0), 70.00 % (P1), 80.00 % (P2) and 83.33 % (P3). Water quality during maintenance were temperature 20.1 – 28.9 0C, pH 6.5 – 7.2, DO 3 – 6.8 mg/L, and ammonia 0.01 – 0.09 mg/L. Masculinization is one method of sex reversal technique that can direct the sex of fish. The aim of this research is to know the effect of different concentration ofcoconut waterto percentage of male guppy fish through immersion method.This research was conducted in August - October 2018 at Fisheries Basic Laboratory, Program Study of Aquaculture, Sriwijaya University. This research used completely randomized design (CRD) with four treatments and three replications i.e without coconut water (P0), coconut water 20%(P1), coconut water 30% (P2) and coconut water 40% (P3). The observed parameters were the percentage of male guppy, survival rate and water quality (temperature, pH, ammonia and dissolved oxigen). The result showed the percentage of male guppy was 46.66 % (P0), 70.00 % (P1), 80.00 % (P2) and 83.33 % (P3). Water quality during maintenance were temperature 20.1 – 28.9 0C, pH 6.5 – 7.2, DO 3 – 6.8 mg/L, and ammonia 0.01 – 0.09 mg/L.

Download Full-text