Biological nitrogen fixation in field pea and vetch: Response to inoculation and residual effect on maize in the Pampean region

2020 ◽  
Vol 115 ◽  
pp. 126016 ◽  
Author(s):  
Juan Martín Enrico ◽  
Carlos Fabian Piccinetti ◽  
Mirian Raquel Barraco ◽  
Maria Belén Agosti ◽  
Roxana Paola Eclesia ◽  
...  
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Residual Effect ◽  
Field Pea ◽  
Pampean Region ◽  
Biological Nitrogen

scholarly journals Mineral Fertilizer Demand for Optimum Biological Nitrogen Fixation and Yield Potentials of Legumes in Northern Ethiopia

2020 ◽  
Vol 12 (16) ◽  
pp. 6449 ◽  
Author(s):  
Shimbahri Mesfin ◽  
Girmay Gebresamuel ◽  
Mitiku Haile ◽  
Amanuel Zenebe ◽  
Girma Desta
Keyword(s):  
Nitrogen Fixation ◽  
Faba Bean ◽  
Biological Nitrogen Fixation ◽  
Cropping Systems ◽  
Mineral Fertilizer ◽  
Field Pea ◽  
Northern Ethiopia ◽  
Soil N ◽  
Combined Application ◽  
Biological Nitrogen

Farmers in Northern Ethiopia integrate legumes in their cropping systems to improve soil fertility. However, biological nitrogen fixation (BNF) potentials of different legumes and their mineral nitrogen (N) and phosphorus (P) demands for optimum BNF and yields are less studied. This study aimed to generate the necessary knowledge to enable development of informed nutrient management recommendations, guide governmental public policy and assist farmer decision making. The experiment was conducted at farmers’ fields with four N levels, three P levels, and three replications. Nodule number and dry biomass per plant were assessed. Nitrogen difference method was used to estimate the amount of fixed N by assuming legume BNF was responsible for differences in plant N and soil mineral N measured between legume treatments and wheat. The result revealed that the highest grain yields of faba bean (2531 kg ha−1), field pea (2493 kg ha−1) and dekeko (1694 kg ha−1) were recorded with the combined application of 20 kg N ha−1 and 20 kg P ha−1. Faba bean, field pea and dekeko also fixed 97, 38 and 49 kg N ha−1, respectively, with the combined application of 20 kg N ha−1 and 20 kg P ha−1; however, lentil fixed 20 kg ha−1 with the combined application of 10 kg N ha−1 and 10 kg P ha−1. The average BNF of legumes in the average of all N and P interaction rates were 67, 23, 32 and 16 kg N ha−1 for faba bean, field pea, dekeko and lentil, respectively. Moreover, faba bean, field pea, dekeko and lentil accumulated a surplus soil N of 37, 21, 26 and 13 kg ha−1, respectively, over the wheat plot. The application of 20 kg N ha−1 and 20 kg P ha−1 levels alone and combined significantly (p < 0.05) increased the nodulation, BNF and yield of legumes; however, 46 kg N ha-1 significantly decreased BNF. This indicated that the combination of 20 kg N ha−1 and 20 kg P ha−1 levels is what mineral fertilizer demands to optimize the BNF and yield of legumes. The results of this study can lead to the development of policy and farmer guidelines, as intensification of the use of legumes supplied with starter N and P fertilizers in Northern Ethiopian cropping systems has the multiple benefits of enhancing inputs of fixed N, improving the soil N status for following crops, and becoming a sustainable option for sustainable soil fertility management practice.

scholarly journals Frequency of field pea in rotations impacts biological nitrogen fixation

2012 ◽  
Vol 92 (6) ◽  
pp. 1005-1011 ◽  
Author(s):  
J. D. Knight
Keyword(s):  
Nitrogen Fixation ◽  
Biological Nitrogen Fixation ◽  
Crop Production ◽  
Crop Residues ◽  
Field Pea ◽  
Continuous Cropping ◽  
Available N ◽  
Lower Productivity ◽  
Soil Inorganic N ◽  
Biological Nitrogen

Knight, J. D. 2012. Frequency of field pea in rotations impacts biological nitrogen fixation. Can. J. Plant Sci. 92: 1005–1011. Economic, environmental and energy concerns about the use of nitrogen (N) fertilizers in crop production have prompted the examination of increasing the frequency of pulses in crop rotations to capitalize on biological nitrogen fixation (BNF). Plots from a field experiment established in 1998 at the Agriculture and Agri-Food Canada Research Farm at Scott, SK, were sampled in 2008, 2009 and 2010. Rotations that included pea every year (continuous pea), every second year (pea-wheat), every third year (pea-canola-wheat) and every fourth year (canola-wheat-pea-wheat) were evaluated for BNF using the enriched15N isotope dilution technique. Nitrogen from BNF in the seed and straw, total above-ground N, seed and straw yield and soil available N and P were evaluated. In 2 of 3 yr, the highest BNF occurred in the two most diverse rotations. Continuous cropping of pea resulted in drastically low BNF in 2008 and 2009. Nitrogen derived from atmosphere in the continuous pea was 15% compared with an average of approximately 55% across all other rotations in these 2 yr. The reduction in BNF was not due to lower productivity in the continuous pea rotation, nor from higher initial soil inorganic N levels inhibiting BNF. In the third year of the study (2010), the more than double the normal precipitation received during the growing season stimulated BNF in pea in the continuous pea rotation. Determining whether the rotation effects on BNF are due to N mineralization of the previous years’ crop residues requires further investigation.

scholarly journals Functional analysis of multiple nifB genes of Paenibacillus strains in synthesis of Mo-, Fe- and V-nitrogenases

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Qin Li ◽  
Haowei Zhang ◽  
Liqun Zhang ◽  
Sanfeng Chen
Keyword(s):  
Functional Analysis ◽  
Nitrogen Fixation ◽  
Gene Cluster ◽  
Biological Nitrogen Fixation ◽  
Deletion Analysis ◽  
Phylogeny Analysis ◽  
Biological Nitrogen

Abstract Background Biological nitrogen fixation is catalyzed by Mo-, V- and Fe-nitrogenases that are encoded by nif, vnf and anf genes, respectively. NifB is the key protein in synthesis of the cofactors of all nitrogenases. Most diazotrophic Paenibacillus strains have only one nifB gene located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV). But some Paenibacillus strains have multiple nifB genes and their functions are not known. Results A total of 138 nifB genes are found in the 116 diazotrophic Paenibacillus strains. Phylogeny analysis shows that these nifB genes fall into 4 classes: nifBI class including the genes (named as nifB1 genes) that are the first gene within the compact nif gene cluster, nifBII class including the genes (named as nifB2 genes) that are adjacent to anf or vnf genes, nifBIII class whose members are designated as nifB3 genes and nifBIV class whose members are named as nifB4 genes are scattered on genomes. Functional analysis by complementation of the ∆nifB mutant of P. polymyxa which has only one nifB gene has shown that both nifB1 and nifB2 are active in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Deletion analysis also has revealed that nifB1 of Paenibacillus sabinae T27 is involved in synthesis of Mo-nitrogenase, while nifB3 and nifB4 genes are not. Complementation of the P. polymyxa ∆nifBHDK mutant with the four reconstituted operons: nifB1anfHDGK, nifB2anfHDGK, nifB1vnfHDGK and nifB2vnfHDGK, has shown both that nifB1 and nifB2 were able to support synthesis of Fe- or V-nitrogenases. Transcriptional results obtained in the original Paenibacillus strains are consistent with the complementation results. Conclusions The multiple nifB genes of the diazotrophic Paenibacillus strains are divided into 4 classes. The nifB1 located in a compact nif gene cluster (nifBHDKENX(orf1)hesAnifV) and the nifB2 genes being adjacent to nif or anf or vnf genes are active in synthesis of Mo-, Fe and V-nitrogenases, but nifB3 and nifB4 are not. The reconstituted anf system comprising 8 genes (nifBanfHDGK and nifXhesAnifV) and vnf system comprising 10 genes (nifBvnfHDGKEN and nifXhesAnifV) support synthesis of Fe-nitrogenase and V-nitrogenase in Paenibacillus background, respectively.

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