scholarly journals Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China

2015 ◽  
Vol 18 (2) ◽  
pp. 96-102 ◽  
Author(s):  
Zhiqiang Mei ◽  
Chun Zhang ◽  
Md. Asaduzzaman Khan ◽  
Ye Zhu ◽  
Mousumi Tania ◽  
...  
Author(s):  
Marwa Hamouda

Abstract Background Silybum marianum L. Gaertn is a medicinal plant of unique pharmaceutical properties in the treatment of liver disorders and diabetic nephropathy. Biochemical (SDS-PAGE) and molecular markers such as randomly amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR) technologies were used in this work to detect genetic diversity of 14 collections of Silybum marianum population in Egypt. Results The electrophoretic pattern of seed protein gave different molecular weight bands, ranging from 24 to 111 KDa with the presence of unique bands. RAPD results revealed a high level of polymorphism (73.2%) using 12 RAPD primers, but only eight of them gave reproducible polymorphic DNA pattern. Sixteen primers were used in the ISSR method; only ten of them yielded clearly identifiable bands. The percentage of polymorphism is about 80% of the studied samples. Conclusion The obtained data confirmed that SDS-protein, RAPD, and ISSR markers are important tools for genetic analysis for Silybum marianum and recommended to give accurate results.


2018 ◽  
Vol 16 (1) ◽  
pp. 153-159
Author(s):  
Prittesh Patel ◽  
B.K. Rajkumar ◽  
Preeti Parmar ◽  
Rusabh Shah ◽  
R. Krishnamurthy

2010 ◽  
Vol 32 (3) ◽  
pp. 491-498 ◽  
Author(s):  
Bhavesh B. Gajera ◽  
Nitish Kumar ◽  
Amritpal S. Singh ◽  
Bhupendra Singh Punvar ◽  
R. Ravikiran ◽  
...  

2006 ◽  
Vol 84 (4) ◽  
pp. 301-307 ◽  
Author(s):  
Jin-Ming Chen ◽  
Wahiti Robert Gituru ◽  
Yu-Hang Wang ◽  
Qing-Feng Wang

2016 ◽  
Vol 30 (4) ◽  
pp. 478-492 ◽  
Author(s):  
Rita Banerjee ◽  
S. Chattopadhyay ◽  
A. K. Saha

2010 ◽  
Vol 54 (1) ◽  
pp. 135-140 ◽  
Author(s):  
B. Sikdar ◽  
M. Bhattacharya ◽  
A. Mukherjee ◽  
A. Banerjee ◽  
E. Ghosh ◽  
...  

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