Role of human papillomavirus (HPV) testing in the follow-up of patients after treatment for cervical precancerous lesions

Introduction: The aim of this study was to examine the role of human papillomavirus testing in the follow-up after treatment for CIN, as a prognostic sign for residual/recurrent cervical precancerous lesions.Methods: A hospital-based analysis was performed on 460 patients previously treated for CIN with cold knife conization, at the University Clinic for Gynecology and Obstetrics and General Hospital Remedika, in Skopje, Republic of Macedonia, in a period of 3 years. The patients were followed-up with HPV testing in addition to cytology, colposcopy and/or biopsy. The first after treatment HPV testing was performed8 months after cold knife conization, proceeded by follow-up within 24 months after treatment, at 4 months intervals.Results: Among 460 treated patients, at the fi rst HPV and cytologic testing, 8 months after treat-ment, 69 (15%) were HPV+, and 391 (85%) HPV negative. From the 69 HPV+ patients, 41 (59.4%) were withcytologic abnormalities and 28 (40.6%) without abnormalities. 12 months after treatment, the number of HPV+ patients developing cytologic abnormalities raised to 45/70 (64.29%). Within the 24 months aftertreatment, the number of patients who had recurrent/ residual CIN from the HPV+ patients reached 50/71 (70.42%); which was 10.87% from all 460 treated patients.Conclusion: Persistence or clearance of HPV especially 8 months after treatment even in patients with normal cytology, is an early valid prognostic marker of treatment failure, and is more accurate than cytologyat the same follow-up intervals.

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Human Papillomavirus Genotyping to Predict the Risk of Cervical Precancerous Lesions or Cancer in Women with Minor Abnormal Cytology in China

Acta Cytologica ◽

10.1159/000441290 ◽

2015 ◽

Vol 59 (5) ◽

pp. 405-411 ◽

Cited By ~ 4

Author(s):

Chun-Qing Lin ◽

Jian-Feng Cui ◽

Xun Zhang ◽

Qin-Jing Pan ◽

Wen Chen ◽

...

Keyword(s):

Human Papillomavirus ◽

Precancerous Lesions ◽

Low Grade ◽

Abnormal Cytology ◽

Cervical Intraepithelial Neoplasia Grade ◽

Cross Sectional ◽

Hpv 16 ◽

Hpv Genotyping ◽

Cervical Precancerous Lesions

Objective: To evaluate the role of human papillomavirus (HPV) genotyping in predicting the risk of cervical precancerous lesions or cancer in women with minor abnormal cytology. Methods and Materials: This study was conducted on 329 women with atypical squamous cells of undetermined significance (ASC-US) and 77 women with low-grade squamous intraepithelial lesions (LSIL) out of a total of 4,215 participants in a multicenter, cross-sectional study. Liquid-based cytology and the Hybrid Capture 2 test (HC2) were used to screen eligible women, and a Linear Array HPV genotyping test was performed on women with positive HC2 results. Results: The sensitivity and specificity for detecting cervical intraepithelial neoplasia grade 2 or worse (CIN2+) based on HPV 16/18 were 82% [95% confidence interval (CI): 52-95%] and 91% (95% CI: 87-94%) in women with ASC-US and 67% (95% CI: 35-88%) and 84% (95% CI: 73-91%) in women with LSIL. The women infected with HPV 16/18 had a significantly higher risk of developing CIN2+ than those infected with other high-risk HPV types in both the ASC-US (OR 9.93, 95% CI: 2.02-48.88) and LSIL (OR 7.45, 95% CI: 1.60-34.68) arms. Conclusions: Genotyping for HPV 16/18 greatly improves specificity, but at the expense of potential sensitivity in the triage of minor cytology abnormalities. The role of genotyping for HPV 16/18 in order to triage women with minor abnormal cytology should be further evaluated in future studies.

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Distribution and attribution of high-risk human papillomavirus genotypes in cervical precancerous lesions in China

Tumor Biology ◽

10.1177/1010428317707373 ◽

2017 ◽

Vol 39 (7) ◽

pp. 101042831770737 ◽

Cited By ~ 1

Author(s):

Wenpeng Wang ◽

Jusheng An ◽

Yan Song ◽

Minjie Wang ◽

Manni Huang ◽

...

Keyword(s):

Human Papillomavirus ◽

High Risk ◽

Precancerous Lesions ◽

Cervical Precancerous Lesions

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Role of Ancillary Techniques in Gynecologic Cytopathology Specimens

Acta Cytologica ◽

10.1159/000496569 ◽

2019 ◽

Vol 64 (1-2) ◽

pp. 63-70

Author(s):

Andrea D. Olivas ◽

Julieta E. Barroeta ◽

Ricardo R. Lastra

Keyword(s):

Cervical Cancer ◽

Human Papillomavirus ◽

Cancer Screening ◽

High Risk ◽

Cervical Cancer Screening ◽

Hpv Testing ◽

Invasive Procedures ◽

Ki 67 ◽

Dual Staining

The association between high-risk genotypes of human papillomavirus (hr-HPV) and cervical cancer is well established. As hr-HPV testing is rapidly becoming a part of routine cervical cancer screening, either in conjunction with cytology or as primary testing, the management of hr-HPV-positive women has to be tailored in a way that increases the detection of cervical abnormalities while decreasing unnecessary colposcopic biopsies or other invasive procedures. In this review, we discuss the overall utility and strategies of hr-HPV testing, as well as the advantages and limitations of potential triage strategies for hr-HPV-positive women, including HPV genotyping, p16/Ki-67 dual staining, and methylation assays.

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Providing high quality information about human papillomavirus for women after treatment for high-grade cervical dysplasia

Sexual Health ◽

10.1071/sh09059 ◽

2010 ◽

Vol 7 (1) ◽

pp. 49 ◽

Cited By ~ 4

Author(s):

Suzanne Dyson ◽

Marian Pitts ◽

Anthony Lyons ◽

Robyn Mullins

Keyword(s):

Human Papillomavirus ◽

Information Needs ◽

Qualitative Interviews ◽

Cervical Dysplasia ◽

Pap Test ◽

Intraepithelial Neoplasia ◽

General Information ◽

High Grade ◽

Hpv Testing

Background: The present study aimed to inform the production of a resource for women who have had a high-grade cervical abnormality and are scheduled to undergo testing for human papillomavirus (HPV) at their 12-month follow-up. Methods: Two rounds of semi-structured, qualitative interviews were held with women who were attending a gynaecological oncology clinic at a major teaching hospital for women in Melbourne, Australia, 6 months after treatment for cervical intraepithelial neoplasia (CIN) to receive a follow-up Pap test and colposcopy. In an initial round of interviews, we gauged the reactions of 16 women to an existing information brochure containing general information about HPV. Based on the findings from the interviews, a second brochure aimed specifically for women scheduled to undergo HPV testing as part of their post treatment follow-up was drafted. Feedback was then gathered from a further 12 women. Results: While all participants had received some information and counselling about HPV and HPV testing as part of their treatment, many still experienced high levels of stress and anxiety about cancer and the sexually transmissible nature of HPV. Many also still had unanswered questions about HPV, their treatment regime and future prognosis. Conclusion: For a brochure to provide an effective adjunct to counselling, it is essential that it is carefully developed and pilot tested to ensure that it is easily understood and meets the information needs of the target audience. Such materials need to provide both medical and psychosocial information about HPV and be presented in accessible, easy to understand language.

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