17β-Estradiol increases endothelial nitric oxide synthase mRNA copy number in cerebral blood vessels: quantification by real-time polymerase chain reaction

2003 ◽  
Vol 478 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Chris Stirone ◽  
Yi Chu ◽  
Lorraine Sunday ◽  
Sue P. Duckles ◽  
Diana N. Krause
Keyword(s):  
Nitric Oxide ◽  
Polymerase Chain Reaction ◽  
Endothelial Nitric Oxide Synthase ◽  
Copy Number ◽  
Cerebral Blood Vessels ◽  
Chain Reaction ◽  
17Β Estradiol ◽  
Polymerase Chain ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

scholarly journals Transient Focal Ischemia Increases Endothelial Nitric Oxide Synthase in Cerebral Blood Vessels

Stroke ◽  
2002 ◽  
Vol 33 (11) ◽  
pp. 2704-2710 ◽  
Author(s):  
Roland Veltkamp ◽  
Nishadi Rajapakse ◽  
Greg Robins ◽  
Michelle Puskar ◽  
Katsuyoshi Shimizu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Blood Vessels ◽  
Endothelial Nitric Oxide Synthase ◽  
Focal Ischemia ◽  
Cerebral Blood Vessels ◽  
Transient Focal Ischemia ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Hypothalamic nitric oxide synthase is depressed in cholestatic rats

1997 ◽  
Vol 272 (5) ◽  
pp. G1034-G1040 ◽  
Author(s):  
M. G. Swain ◽  
T. Le ◽  
A. W. Tigley ◽  
P. Beck
Keyword(s):  
Nitric Oxide ◽  
Polymerase Chain Reaction ◽  
Steady State ◽  
Nitric Oxide Synthase ◽  
Mrna Levels ◽  
Chain Reaction ◽  
Bile Duct Resection ◽  
Protein Levels ◽  
Polymerase Chain ◽  
Oxide Synthase

We examined hypothalamic nitric oxide synthase (NOS) levels and release as well as steady-state mRNA levels in rats with cholestasis due to bile duct resection (BDR) and in sham-resected control rats. BDR rats had a significant reduction in hypothalamic NOS-containing neurons in the hypothalamic paraventricular nucleus as determined by NADPH-diaphorase staining, compared with sham-resected controls. In addition, NOS activity, measured indirectly by determining nitrite release from hypothalamic explants, was significantly lower in BDR rats compared with sham-resected animals. Hypothalamic steady-state NOS mRNA levels [brain constitutive NOS (bNOS)] were determined by semiquantitative reverse transcription-polymerase chain reaction and were found to be increased 1.5-fold in BDR rats compared with sham rats. In summary, BDR rats have diminished hypothalamic NOS levels and activity coupled with enhanced steady-state bNOS mRNA levels, suggesting that depressed hypothalamic NOS protein levels are due to posttranscriptional defects.

scholarly journals Estrogen Increases Endothelial Nitric Oxide Synthase via Estrogen Receptors in Rat Cerebral Blood Vessels

Stroke ◽  
2002 ◽  
Vol 33 (6) ◽  
pp. 1685-1691 ◽  
Author(s):  
Anne Marie McNeill ◽  
Chunying Zhang ◽  
Frank Z. Stanczyk ◽  
Sue P. Duckles ◽  
Diana N. Krause
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Estrogen Receptors ◽  
Blood Vessels ◽  
Endothelial Nitric Oxide Synthase ◽  
Cerebral Blood Vessels ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide
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