High performance glucose/O2 compartment-less biofuel cell using DNA/CNTs as platform for immobilizing bilirubin oxidase as novel biocathode and integrated NH2-CNTs/dendrimer/glucose dehydrogenase/nile blue as bioanode

Recent studies have focused on tailoring the catalytic currents of multicopper oxidase (MCO) enzymes-based biocathodes to enhance oxygen reduction. Biocathodes modified with natural substrates specific for MCO enzymes demonstrated drastic improvement for oxygen reduction. Performance of 1-pyrenebutanoic acid, succinimidyl ester (PBSE), and 2,5-dimethyl-1-phenyl-1H-pyrrole-3-carbaldehyde (Di-Carb) oriented bilirubin oxidase (BOx) modified gas diffusion biocathode has been highly improved by incorporating hematin, a porphyrin precursor as electron transfer enhancement moiety. Hematin modified electrodes demonstrated direct electron transfer reaction of BOx exhibiting larger O2 reduction in current density in phosphate buffer solution (pH 7.0) without the need of a mediator. A remarkable improvement in the catalytic currents with 2.5-fold increase compared to non-hematin modified oriented BOx electrodes was achieved. Moreover, a mediatorless and compartmentless glucose/O2 biofuel cell based on DET-type bioelectrocatalysis via the BOx cathode and the glucose dehydrogenase (GDH) anode demonstrated peak power densities of 1 mW/cm2 at pH 7.0 with 100 mM glucose/10 mM NAD fuel. The maximum current density of 1.6 mA/cm2 and the maximum power density of 0.4 mW/cm2 were achieved at 300 mV with nonmodified BOx cathode, while 3.5 mA/cm2 and 1.1 mW/cm2 of current and power density were achieved with hematin modified cathode. The performance improved 2.4 times which attributes to the hematin acting as a natural precursor and activator for BOx activity enhancement.

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Flexible and Stretchable Enzymatic Biofuel Cell with High Performance Enabled by Textile Electrodes and Polymer Hydrogel Electrolyte

Nano Letters ◽

10.1021/acs.nanolett.1c03621 ◽

2021 ◽

Author(s):

Zilin Chen ◽

Yao Yao ◽

Tian Lv ◽

Yunlong Yang ◽

Yanan Liu ◽

...

Keyword(s):

High Performance ◽

Biofuel Cell ◽

Polymer Hydrogel ◽

Enzymatic Biofuel Cell ◽

Textile Electrodes ◽

Polymer Hydrogel Electrolyte

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High-performance non-enzymatic catalysts based on 3D hierarchical hollow porous Co3O4 nanododecahedras in situ decorated on carbon nanotubes for glucose detection and biofuel cell application

Analytical and Bioanalytical Chemistry ◽

10.1007/s00216-018-0875-3 ◽

2018 ◽

Vol 410 (7) ◽

pp. 2019-2029 ◽

Cited By ~ 7

Author(s):

Shiyue Wang ◽

Xiaohua Zhang ◽

Junlin Huang ◽

Jinhua Chen

Keyword(s):

Carbon Nanotubes ◽

High Performance ◽

Biofuel Cell ◽

Glucose Detection

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Three-Dimensional Glucose/Oxygen Biofuel Cells Based on Enzymes Embedded in Tetrabutylammonium Modified Nafion

Journal of Electrochemical Energy Conversion and Storage ◽

10.1115/1.4049926 ◽

2021 ◽

Vol 18 (4) ◽

Author(s):

Yuchen Hui ◽

Xiaoyan Ma ◽

Rong Cai ◽

Shelley D. Minteer

Keyword(s):

Direct Electron Transfer ◽

Glucose Dehydrogenase ◽

Tetrabutylammonium Bromide ◽

Three Dimensional ◽

Phosphate Buffer Solution ◽

Open Circuit ◽

Biofuel Cell ◽

Buffer Solution ◽

Multi Wall Carbon Nanotubes ◽

Enzymatic Biofuel Cell

Abstract A stable three-dimensional glucose/oxygen enzymatic biofuel cell is fabricated based on the method of polymer encapsulation-based immobilization. And three-dimensional carbon felt is used as the substrate of the bio-electrode for increasing enzymatic loading density. Gold nanoparticles and multi-wall carbon nanotubes are employed to promote direct electron transfer and enhance conductivity and electron conduction rate of bio-electrodes. Glucose dehydrogenase and bilirubin oxidase are immobilized with tetrabutylammonium bromide (TBAB) modified Nafion, which enhances the stability of the bio-electrodes by the immobilization method. A membrane-free glucose/oxygen biofuel cell is assembled with a high open-circuit voltage of 0.85 V and a maximum power density of 21.9 ± 0.1 μW/cm2 in 0.1 M pH 7.0 phosphate buffer solution with 100 mM glucose and air saturation. And the biofuel cell shows high stability to the condition. After 60 days of periodic storage experiments, the performance of the enzymatic biofuel cell still maintained 90.3% of its electrochemical performance.

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A Self-Powered Biosensor for the Detection of Glutathione

Biosensors ◽

10.3390/bios10090114 ◽

2020 ◽

Vol 10 (9) ◽

pp. 114 ◽

Cited By ~ 2

Author(s):

Brandon G. Roy ◽

Julia L. Rutherford ◽

Anna E. Weaver ◽

Kevin Beaver ◽

Michelle Rasmussen

Keyword(s):

Detection Limit ◽

Glucose Oxidase ◽

Power Output ◽

Low Cost ◽

Linear Range ◽

Biofuel Cell ◽

Bilirubin Oxidase ◽

Biological Molecule ◽

Enzymatic Biofuel Cell ◽

Self Powered

Glutathione is an important biological molecule which can be an indicator of numerous diseases. A method for self-powered detection of glutathione levels in solution has been developed using an enzymatic biofuel cell. The device consists of a glucose oxidase anode and a bilirubin oxidase cathode. For the detection of glutathione, the inhibition of bilirubin oxidase leads to a measurable decrease in current and power output. The reported method has a detection limit of 0.043 mM and a linear range up to 1.7 mM. Being able to detect a range of concentrations can be useful in evaluating a patient’s health. This method has the potential to be implemented as a quick, low-cost alternative to previously reported methods.

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Development of Biofuel Cell Using a Complex of Highly Oriented Immobilized His-Tagged Enzyme and Carbon Nanotube Surface Through a Pyrene Derivative

Journal of Nanoscience and Nanotechnology ◽

10.1166/jnn.2019.16121 ◽

2019 ◽

Vol 19 (6) ◽

pp. 3551-3557 ◽

Cited By ~ 4

Author(s):

Hiroaki Sakamoto ◽

Ayako Koto ◽

Ei-Ichiro Takamura ◽

Hitoshi Asakawa ◽

Takeshi Fukuma ◽

...

Keyword(s):

Electron Transfer ◽

Carbon Nanotube ◽

Glucose Dehydrogenase ◽

Molecular Layer ◽

Biofuel Cells ◽

Biofuel Cell ◽

Similar Process ◽

Enzyme Complex ◽

Catalytic Current ◽

Oriented Immobilization

For increasing the output of biofuel cells, increasing the cooperation between enzyme reaction and electron transfer on the electrode surface is essential. Highly oriented immobilization of enzymes onto a carbon nanotube (CNT) with a large specific surface area and excellent conductivity would increase the potential for their application as biosensors and biofuel cells, by utilizing the electron transfer between the electrode-molecular layer. In this study, we prepared a CNT-enzyme complex with highly oriented immobilization of enzyme onto the CNT surface. The complex showed excellent electrical characteristics, and could be used to develop biodevices that enable efficient electron transfer. Multi-walled carbon nanotubes (MWCNT) were dispersed by pyrene butyric acid N-hydroxysuccinimide ester, and then N-(5-amino-1-carboxypentyl) iminodiacetic acid (AB-NTA) and NiCl2 were added to modify the NTA-Ni2+ complex on the CNT surface. Pyrroloquinoline quinone (PQQ)-dependent glucose dehydrogenase (GDH) was immobilized on the CNT surface through a genetically introduced His-tag. Formation of the MWCNT-enzyme complex was confirmed by monitoring the catalytic current electrochemically to indicate the enzymatic activity. PQQ-GDH was also immobilized onto a highly ordered pyrolytic graphite surface using a similar process, and the enzyme monolayer was visualized by atomic force microscopy to confirm its structural properties. A biofuel cell was constructed using the prepared CNT-enzyme complex and output evaluation was carried out. As a result, an output of 32 μW/cm2 could be obtained without mediators.

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