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In this study, it was aimed to develop a reliable tool based on star-shaped poly(glycidyl
methacrylate) polymer coated disposable indium tin oxide electrode for determination of Tumor necrosis
factor-alpha (TNF α), an important disease biomarker. Star shaped polymer was used as an interface
material for anti-TNF α antibodies immobilization. Anti-TNF α antibodies were immobilized covalently
onto polymer coated indium tin oxide electrode. The immunosensor responses were recorded by
electrochemical impedance spectroscopy and cyclic voltammetry techniques and electrochemical
impedance spectroscopy was utilized for TNF α determination. The suggested immunosensor exhibited a
linear range between 0.02 and 4 pg/mL TNF α, and the detection limit was found as 6 fg/mL. Scanning
electron microscopy and atomic force microscopy were used for electrode surface characterization. In
addition, the suggested immunosensor was used for TNF α sensing in human serum samples. The results
displayed recoveries between 97.07 and 100.19%. Moreover, this immunosensor had a simple fabrication
procedure and a long storage-stability.