Aluminum toxicity-induced pollen tube growth inhibition in apple (Malus domestica) is mediated by interrupting calcium dynamics and modification of cell wall components

2020 ◽  
Vol 171 ◽  
pp. 103928
Author(s):  
Kefeng Fang ◽  
Pengxue Xie ◽  
Qing Zhang ◽  
Yu Xing ◽  
Qingqin Cao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Growth Inhibition ◽  
Malus Domestica ◽  
Pollen Tube Growth ◽  
Aluminum Toxicity ◽  
Tube Growth ◽  
Calcium Dynamics ◽  
Cell Wall Components ◽  
Wall Components

scholarly journals Exocyst mutants suppress pollen tube growth and cell wall structural defects of hydroxyproline O‐arabinosyltransferase mutants

2020 ◽  
Vol 103 (4) ◽  
pp. 1399-1419
Author(s):  
Steven Beuder ◽  
Alexandria Dorchak ◽  
Ashwini Bhide ◽  
Svenning Rune Moeller ◽  
Bent L. Petersen ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Structural Defects

Cellular Mechanisms for Pollen Tube Growth Inhibition in Gametophytic Self-incompatibility

2006 ◽  
pp. 201-221 ◽  
Author(s):  
Barend H. J. de Graaf ◽  
Chris Lee ◽  
Bruce A. McClure ◽  
Noni (V. E.) Franklin-Tong
Keyword(s):  
Pollen Tube ◽  
Growth Inhibition ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Cellular Mechanisms

scholarly journals To preserve or to destroy, that is the question: the role of the cell wall integrity pathway in pollen tube growth

2019 ◽  
Vol 52 ◽  
pp. 131-139 ◽  
Author(s):  
Hannes Vogler ◽  
Gorka Santos-Fernandez ◽  
Martin A Mecchia ◽  
Ueli Grossniklaus
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Cell Wall Integrity ◽  
Tube Growth ◽  
Cell Wall Integrity Pathway

scholarly journals Inhibition of Apoplastic Calmodulin Impairs Calcium Homeostasis and Cell Wall Modeling during Cedrus deodara Pollen Tube Growth

PLoS ONE ◽  
2013 ◽  
Vol 8 (2) ◽  
pp. e55411 ◽  
Author(s):  
Li Wang ◽  
Xueqin Lv ◽  
Hong Li ◽  
Min Zhang ◽  
Hong Wang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Pollen Tube ◽  
Calcium Homeostasis ◽  
Pollen Tube Growth ◽  
Tube Growth ◽  
Cedrus Deodara

Malus domestica ADF1 severs actin filaments in growing pollen tubes

2017 ◽  
Vol 44 (4) ◽  
pp. 455 ◽  
Author(s):  
Qing Yang ◽  
ShengNan Wang ◽  
ChuanBao Wu ◽  
QiuLei Zhang ◽  
Yi Zhang ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Actin Cytoskeleton ◽  
Malus Domestica ◽  
Pollen Tube Growth ◽  
Actin Filaments ◽  
Germination Rate ◽  
Tube Growth ◽  
Sequence Alignments ◽  
Gene Encoding ◽  
Domain Structures

A dynamic actin cytoskeleton is essential for pollen tube growth and germination. However, the molecular mechanism that determines the organisation of the actin cytoskeleton in pollen remains poorly understood. ADF modulates the structure and dynamics of actin filaments and influences the higher-order organisation of the actin cytoskeleton in eukaryotic cells. Members of the ADF family have been shown to have important functions in pollen tube growth. However, the role of this gene family remains largely unknown in apple (Malus domestica Borkh.). In this study, we identified seven ADFs in the apple genome. Phylogenetic analysis showed that MdADF1 clusters with Arabidopsis thaliana (L.) Heynh. AtADF7, ADF8, ADF10 and AtADF11. We performed sequence alignments and analysed the domain structures of the seven MdADF proteins and identified the chromosome locations of the encoding genes. We cloned the gene encoding MdADF1 from ‘Ralls Janet’ apple and found that it was strongly expressed in pollen. Biochemical assays revealed that MdADF1 directly bound to and severed F-actin under low Ca2+ conditions. We demonstrated that knockdown of MdADF1 inhibited pollen tube growth and reduced the pollen germination rate, but rendered the pollen insensitive to treatment with Latrunculin B, an actin depolymerising agent. Taken together, our results provide insight into the function of MdADF1 and serve as a reference for studies of ADF in other plants.

Boron deficiency alters cytosolic Ca2+ concentration and affects the cell wall components of pollen tubes in Malus domestica

Plant Biology ◽  
2018 ◽  
Vol 21 (2) ◽  
pp. 343-351 ◽  
Author(s):  
K. F. Fang ◽  
B. S. Du ◽  
Q. Zhang ◽  
Y. Xing ◽  
Q. Q. Cao ◽  
...  
Keyword(s):  
Cell Wall ◽  
Malus Domestica ◽  
Pollen Tubes ◽  
Boron Deficiency ◽  
Cell Wall Components ◽  
Wall Components
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