Copper regulation and homeostasis of Daphnia magna and Pseudokirchneriella subcapitata: influence of acclimation

Abstract For the prediction of metals mixture ecotoxicity, the BLM approach is promising since it evaluates the amount of metals accumulated on the biotic ligand on the basis of water chemistry, i.e. species (major cations) competing with metals, and related toxicity. Based on previous work by Farley et al. 2015 (MMME research project), this study aimed at modelling toxicity of Cd:Cu mixtures (0:1–1:1–1:0–1:2 − 1:3 − 2:1–3:1–4:1–5:1–6:1) to the crustacean Daphnia magna (48h immobilization tests) and the microalga Pseudokirchneriella subcapitata (72h growth inhibition tests). The USGS model was chosen, assuming additivity of effects and accumulation of metals on a single site. The assumption that EDTA could contribute to toxicity through metals complexing was also tested, and potential effects due to reduction of ions Ca2+ absorption by metals were considered. Modelling started with parameter values of Farley et al. 2015 and some of these parameters were adjusted to fit modelled data on observed data. The results show that toxicity can be correctly predicted for the microalgae and that the hypothesis of additivity is verified. For daphnids, the prediction was roughly correct, but taking into account CuEDTA led to more realistic parameter values close to that reported by Farley et al. 2015. However, It seems that, for daphnids responses, metals interact either antagonistically or synergistically depending on the Cu:Cd ratio. Furthermore, synergy could not be explained by additional effects linked to a reduction of Ca absorption since this reduction, mainly due to Cd, increased inversely to synergy. Finally, the USGS model applied to our data was able to predict Cu:Cd mixture toxicity to microalgae and daphnids, giving rise to estimated EC50s roughly reflecting EC50s calculated from observed toxicity.

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Assessment of acrylamide toxicity using a battery of standardised bioassays / Procjena toksičnosti akrilamida pomoću standardiziranih biotestova

Archives of Industrial Hygiene and Toxicology ◽

10.1515/aiht-2015-66-2715 ◽

2015 ◽

Vol 66 (4) ◽

pp. 315-321 ◽

Cited By ~ 6

Author(s):

Mira Zovko ◽

Željka Vidaković-Cifrek ◽

Želimira Cvetković ◽

Jasna Bošnir ◽

Sandra Šikić

Keyword(s):

Daphnia Magna ◽

Lemna Minor ◽

Low Cost ◽

Drinking Water Treatment ◽

Pseudokirchneriella Subcapitata ◽

Laboratory Animals ◽

Freshwater Organisms ◽

Desmodesmus Subspicatus ◽

Class 1 ◽

Increased Sensitivity

Acrylamide is a monomer widely used as an intermediate in the production of organic chemicals, e.g. polyacrylamides (PAMs). Since PAMs are low cost chemicals with applications in various industries and waste- and drinking water treatment, a certain amount of non-polymerised acrylamide is expected to end up in waterways. PAMs are non-toxic but acrylamide induces neurotoxic effects in humans and genotoxic, reproductive, and carcinogenic effects in laboratory animals. In order to evaluate the effect of acrylamide on freshwater organisms, bioassays were conducted on four species: algae Desmodesmus subspicatus and Pseudokirchneriella subcapitata, duckweed Lemna minor and water flea Daphnia magna according to ISO (International Organization for Standardisation) standardised methods. This approach ensures the evaluation of acrylamide toxicity on organisms with different levels of organisation and the comparability of results, and it examines the value of using a battery of low-cost standardised bioassays in the monitoring of pollution and contamination of aquatic ecosystems. These results showed that EC50 values were lower for Desmodesmus subspicatus and Pseudokirchneriella subcapitata than for Daphnia magna and Lemna minor, which suggests an increased sensitivity of algae to acrylamide. According to the toxic unit approach, the values estimated by the Lemna minor and Daphnia magna bioassays, classify acrylamide as slightly toxic (TU=0-1; Class 1). The results obtained from algal bioassays (Desmodesmus subspicatus and Pseudokirchneriella subcapitata) revealed the toxic effect of acrylamide (TU=1-10; Class 2) on these organisms.

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Construction of a Simple Bi-trophic Microcosm System Using Standard Test Species (Pseudokirchneriella subcapitata and Daphnia magna) for Testing Chemical Toxicities

Korean Journal of Ecology and Environment ◽

10.11614/ksl.2016.49.3.228 ◽

2016 ◽

Vol 49 (3) ◽

pp. 228-235

Author(s):

Masaki Sakamoto ◽

Hiroyuki Mano ◽

Takayuki Hanazato ◽

Kwang-Hyeon Chang

Keyword(s):

Daphnia Magna ◽

Standard Test ◽

Pseudokirchneriella Subcapitata ◽

Test Species

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Analyzing the capacity of the Daphnia magna and Pseudokirchneriella subcapitata bioavailability models to predict chronic zinc toxicity at high pH and low calcium concentrations and formulation of a generalized bioavailability model for D. magna

Environmental Toxicology and Chemistry ◽

10.1002/etc.3840 ◽

2017 ◽

Vol 36 (10) ◽

pp. 2781-2798 ◽

Cited By ~ 4

Author(s):

Tina Van Regenmortel ◽

Olivier Berteloot ◽

Colin R. Janssen ◽

Karel A. C. De Schamphelaere

Keyword(s):

Daphnia Magna ◽

Pseudokirchneriella Subcapitata ◽

Zinc Toxicity ◽

High Ph ◽

Low Calcium

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Acute toxicity of ionic liquids for three freshwater organisms: Pseudokirchneriella subcapitata, Daphnia magna and Danio rerio

Ecotoxicology and Environmental Safety ◽

10.1016/j.ecoenv.2008.09.010 ◽

2009 ◽

Vol 72 (4) ◽

pp. 1170-1176 ◽

Cited By ~ 203

Author(s):

Carlo Pretti ◽

Cinzia Chiappe ◽

Ilaria Baldetti ◽

Sara Brunini ◽

Gianfranca Monni ◽

...

Keyword(s):

Ionic Liquids ◽

Acute Toxicity ◽

Daphnia Magna ◽

Danio Rerio ◽

Pseudokirchneriella Subcapitata ◽

Freshwater Organisms

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AVALIAÇÃO DA TOXIDADE DA ÁGUA E SEDIMENTO DO AÇUDE JABURU (CEARÁ-BR) NA REGIÃO DO SEMI-ÁRIDO

Holos Environment ◽

10.14295/holos.v12i2.5282 ◽

2012 ◽

Vol 12 (2) ◽

pp. 168

Author(s):

Priscila Maria Dellamatrice ◽

Rinaldo Dos Santos Araújo ◽

Lilian Da Silva Costa ◽

Milena Viana De Sousa

Keyword(s):

Daphnia Magna ◽

Lactuca Sativa ◽

Pseudokirchneriella Subcapitata

Os açudes são o único recurso disponível para abastecimento de água da população na região semi-árida durante a estação seca. Esse recurso, porém, também é utilizado na agricultura desenvolvida no entorno do açude, para irrigação. Devido ao uso contínuo de fertilizantes e pesticidas, esses produtos podem ser carreados para as águas, poluindo-as. O Açude Jaburu, localizado na região noroeste do Estado do Ceará, é responsável pelo abastecimento de oito cidades e oito vilas e distritos. Nele, foram realizados testes de toxicidade aguda na água e sedimento, utilizando os organismos Daphnia magna, a alga Pseudokirchneriella subcapitata e sementes de alface (Lactuca sativa). Foi também realizado o teste crônico na água com Daphnia magna. Foram analisados três pontos em três diferentes profundidades para verificar efeito da estratificação, nos períodos de seca e cheia. Toxicidade aguda da água não foi detectada em nenhum dos pontos amostrados. No teste de toxicidade crônica, foi detectada toxicidade nos dois períodos, seca e cheia, sendo mais pronunciado no primeiro. No sedimento, houve toxicidade aguda somente no período de seca para D. magna, enquanto para os outros organismos não houve toxicidade. Os resultados demonstraram que o Açude Jaburu ainda não se encontra severamente impactado, porém os testes de toxicidade crônica mostram que pode estar sofrendo poluição por compostos químicos em baixas concentrações e poderá causar dano em longo prazo. Propõe-se um melhor gerenciamento ambiental desse recurso, visando mitigar os impactos ambientais negativos nesse ecossistema.

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