Characterization and site-directed mutagenesis of an α-galactosidase from the deep-sea bacterium Bacillus megaterium

2014 ◽  
Vol 56 ◽  
pp. 46-52 ◽  
Author(s):  
Haibo Xu ◽  
Yongjun Qin ◽  
Zongqing Huang ◽  
Ziduo Liu
Keyword(s):  
Bacillus Megaterium ◽  
Deep Sea ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Bacterium Bacillus

Increasing synthetic performance of penicillin G acylase from Bacillus megaterium by site-directed mutagenesis

2007 ◽  
Vol 74 (5) ◽  
pp. 1023-1030 ◽  
Author(s):  
Jingang Wang ◽  
Qing Zhang ◽  
He Huang ◽  
Zhongyi Yuan ◽  
Dafu Ding ◽  
...  
Keyword(s):  
Bacillus Megaterium ◽  
Site Directed Mutagenesis ◽  
Penicillin G Acylase ◽  
Penicillin G ◽  
Directed Mutagenesis

scholarly journals Identification of a Receptor Subunit and Putative Ligand-Binding Residues Involved in the Bacillus megaterium QM B1551 Spore Germination Response to Glucose

2010 ◽  
Vol 192 (17) ◽  
pp. 4317-4326 ◽  
Author(s):  
Graham Christie ◽  
Hansjörg Götzke ◽  
Christopher R. Lowe
Keyword(s):  
Bacillus Megaterium ◽  
Molecular Basis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Protein Subunit ◽  
Aromatic Residues ◽  
Apparent Affinity ◽  
The Third ◽  
Binding Residues ◽  
Putative Ligand Binding

ABSTRACT The molecular basis for the recognition of glucose as a germinant molecule by spores of Bacillus megaterium QM B1551 has been examined. A chromosome-located locus (BMQ_1820, renamed gerWB) is shown to encode a receptor B-protein subunit that interacts with the GerUA and GerUC proteins to form a receptor that is cognate for both glucose and leucine. GerWB represents the third receptor B protein that binds to glucose in this strain. Site-directed mutagenesis (SDM) experiments conducted on charged proline and aromatic residues predicted to reside in the transmembrane domains of a previously characterized receptor B protein, GerVB, reveal the importance to receptor function of a cluster of residues predicted to reside in the middle of the transmembrane 6 (TM6) domain. Reductions in the region of 70- to 165-fold in the apparent affinity of receptors for glucose in which Glu196, Tyr191, and Phe192 are individually replaced by SDM indicate that some or all of these residues may be directly involved in the binding of glucose and perhaps other germinants to the germinant receptor.

The site-directed mutagenesis and prokaryotic expression of midkine gene in Cynoglossus semilaevis

2013 ◽  
Vol 37 (3) ◽  
pp. 330
Author(s):  
Yanan WANG ◽  
Xudong LIU ◽  
Linlin MU ◽  
Zhipeng LIU ◽  
Chunmei LI ◽  
...  
Keyword(s):  
Prokaryotic Expression ◽  
Cynoglossus Semilaevis ◽  
Site Directed Mutagenesis ◽  
Directed Mutagenesis

Site-directed mutagenesis on alkaline phosphatasefrompseudomonas aeruginosapao1 for enhanced activity

2016 ◽  
Vol 7 (4) ◽  
Author(s):  
UMA SELVARAJ ◽  
THIRUMALAI MUTHUKUMARESAN ◽  
GAYATHRI VIJAYENDRAN ◽  
SENTHIL KUMAR DEVAN ◽  
VENU BABU P ◽  
...  
Keyword(s):  
Site Directed Mutagenesis ◽  
Directed Mutagenesis ◽  
Enhanced Activity
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