High-level production of linalool by engineered Saccharomyces cerevisiae harboring dual mevalonate pathways in mitochondria and cytoplasm

2020 ◽  
Vol 134 ◽  
pp. 109462 ◽  
Author(s):  
Yaoyao Zhang ◽  
Jin Wang ◽  
Xianshuang Cao ◽  
Wei Liu ◽  
Hongwei Yu ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Engineered Saccharomyces Cerevisiae ◽  
High Level ◽  
Level Production

Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae for high-level production of isoprenoids

2007 ◽  
Vol 9 (2) ◽  
pp. 160-168 ◽  
Author(s):  
Yoichiro Shiba ◽  
Eric M. Paradise ◽  
James Kirby ◽  
Dae-Kyun Ro ◽  
Jay D. Keasling
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Pyruvate Dehydrogenase ◽  
High Level ◽  
Level Production

scholarly journals Establishing a synthetic pathway for high-level production of 3-hydroxypropionic acid in Saccharomyces cerevisiae via β-alanine

2015 ◽  
Vol 27 ◽  
pp. 57-64 ◽  
Author(s):  
Irina Borodina ◽  
Kanchana R. Kildegaard ◽  
Niels B. Jensen ◽  
Thomas H. Blicher ◽  
Jérôme Maury ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Synthetic Pathway ◽  
High Level ◽  
Hydroxypropionic Acid ◽  
Level Production

Multiple-yapsin-deficient mutant strains for high-level production of intact recombinant proteins in Saccharomyces cerevisiae

2010 ◽  
Vol 149 (1-2) ◽  
pp. 1-7 ◽  
Author(s):  
Eun Young Cho ◽  
Seon Ah Cheon ◽  
Hyunah Kim ◽  
Jinho Choo ◽  
Dong-Jik Lee ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Recombinant Proteins ◽  
Deficient Mutant ◽  
Mutant Strains ◽  
High Level ◽  
Level Production

scholarly journals Metabolic engineering of Saccharomyces cerevisiae for production of very long chain fatty acid-derived chemicals

2017 ◽  
Vol 8 (1) ◽  
Author(s):  
Tao Yu ◽  
Yongjin J. Zhou ◽  
Leonie Wenning ◽  
Quanli Liu ◽  
Anastasia Krivoruchko ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fatty Acid ◽  
Cell Growth ◽  
Control Strategy ◽  
Dynamic Control ◽  
Chain Fatty Acid ◽  
Long Chain Fatty Acid ◽  
High Level ◽  
Level Production ◽  
Long Chain

Abstract Production of chemicals and biofuels through microbial fermentation is an economical and sustainable alternative for traditional chemical synthesis. Here we present the construction of a Saccharomyces cerevisiae platform strain for high-level production of very-long-chain fatty acid (VLCFA)-derived chemicals. Through rewiring the native fatty acid elongation system and implementing a heterologous Mycobacteria FAS I system, we establish an increased biosynthesis of VLCFAs in S. cerevisiae. VLCFAs can be selectively modified towards the fatty alcohol docosanol (C22H46O) by expressing a specific fatty acid reductase. Expression of this enzyme is shown to impair cell growth due to consumption of VLCFA-CoAs. We therefore implement a dynamic control strategy for separating cell growth from docosanol production. We successfully establish high-level and selective docosanol production of 83.5 mg l−1 in yeast. This approach will provide a universal strategy towards the production of similar high value chemicals in a more scalable, stable and sustainable manner.

scholarly journals High-level production of animal-free recombinant transferrin from saccharomyces cerevisiae

2010 ◽  
Vol 9 (1) ◽  
pp. 87 ◽  
Author(s):  
Christopher JA Finnis ◽  
Tom Payne ◽  
Joanna Hay ◽  
Neil Dodsworth ◽  
Diane Wilkinson ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
High Level ◽  
Level Production

Proteinase B Disruption Is Required for High Level Production of Human Mechano-Growth Factor in Saccharomyces cerevisiae

2010 ◽  
Vol 18 (3) ◽  
pp. 188-194 ◽  
Author(s):  
Elena V. Morozkina ◽  
Alexey N. Marchenko ◽  
Jan S. Keruchenko ◽  
Irina D. Keruchenko ◽  
Vyacheslav P. Khotchenkov ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Growth Factor ◽  
Mechano Growth Factor ◽  
High Level ◽  
Level Production
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