Magnetic thermosensitive polymer composite carrier with target spacing for enhancing immobilized enzyme performance

2021 ◽  
pp. 109896
Author(s):  
Hongyi Tu ◽  
Boyuan Zhang ◽  
Xiayun Zhang ◽  
Chunli Zhao ◽  
Lin Li ◽  
...  
Keyword(s):  
Polymer Composite ◽  
Immobilized Enzyme ◽  
Thermosensitive Polymer ◽  
Target Spacing ◽  
Composite Carrier

scholarly journals Switchable dispersivity and molecular-trapping performance of mesostructured CaCO3–thermosensitive polymer composite microspheres

2015 ◽  
Vol 3 (17) ◽  
pp. 3604-3608 ◽  
Author(s):  
Atsuharu Inoue ◽  
Hitoshi Tamagawa ◽  
Yuya Oaki ◽  
Sadahito Aoshima ◽  
Hiroaki Imai
Keyword(s):  
Polymer Composite ◽  
Thermosensitive Polymer ◽  
Composite Microspheres ◽  
Trapping Performance

Dispersivity and molecular-trapping performance of mesostructured CaCO3–thermosensitive polymer composite microspheres were switched by a swing in temperature.

On the Preparation of Bovine α-Thrombin

1978 ◽  
Vol 39 (01) ◽  
pp. 193-200 ◽  
Author(s):  
Erwin F Workman ◽  
Roger L Lundblad
Keyword(s):  
Immobilized Enzyme ◽  
Catalytic Properties ◽  
Specific Activity ◽  
Guanidine Hydrochloride ◽  
Low Molecular Weight ◽  
Reversible Process ◽  
Gel Beads ◽  
Tissue Thromboplastin ◽  
C 50 ◽  
Hydrolysis Of

SummaryAn improved method for the preparation of bovine α-thrombin is described. The procedure involves the activation of partially purified prothrombin with tissue thromboplastin followed by chromatography on Sulfopropyl-Sephadex C-50. The purified enzyme is homogeneous on polyacrylamide discontinuous gel electrophoresis and has a specific activity toward fibrinogen of 2,200–2,700 N.I.H. U/mg. Its stability on storage in liquid media is dependent on both ionic strenght and temperature. Increasing ionic strength and decreasing temperature result in optimal stability. The denaturation of α-thrombin by guanidine hydrochloride was found to be a partially reversible process with the renatured species possessing properties similar to “aged” thrombin. In addition, the catalytic properties of a-thrombin covalently attached to agarose gel beads were also examined. The activity of the immobilized enzyme toward fibrinogen was affected to a much greater extent than was the hydrolysis of low molecular weight, synthetic substrates.

FSUE "VIAM" solvent-free binders for polymer composite materials

2016 ◽  
Vol 2 (2) ◽  
pp. 37-42 ◽  
Author(s):  
E. N. Kablov ◽  
L. V. Chursova ◽  
A. N. Babin ◽  
R. R. Mukhametov ◽  
N. N. Panina
Keyword(s):  
Composite Materials ◽  
Polymer Composite ◽  
Solvent Free ◽  
Polymer Composite Materials
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