Removal of Pb and Hg from marine intertidal sediment by using rhamnolipid biosurfactant produced by a Pseudomonas aeruginosa strain

2021 ◽  
Vol 22 ◽  
pp. 101456
Author(s):  
Qingguo Chen ◽  
Yijing Li ◽  
Mei Liu ◽  
Baikang Zhu ◽  
Jun Mu ◽  
...  
2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.


2002 ◽  
Vol 18 (6) ◽  
pp. 1277-1281 ◽  
Author(s):  
K.S.M. Rahman ◽  
T.J. Rahman ◽  
S. McClean ◽  
R. Marchant ◽  
I.M. Banat

2012 ◽  
Vol 77 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Milena Rikalovic ◽  
Gordana Gojgic-Cvijovic ◽  
Miroslav Vrvic ◽  
Ivanka Karadzic

Production and characterization of rhamnolipid biosurfactant obtained by strain Pseudomonas aeruginosa san ai was investigated. With regard to carbon and nitrogen source several media were tested to enhance production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sun flower oil as a source of carbon and mineral ammonium chloride and peptone as a nitrogen source greatly improved rhamnolipid production, from 0.15 on basic PPAS (C/N ratio 4.0), to 3 g L-1, on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing effect of three factors: temperature, concentration of carbon and nitrogen source (w/w), in optimized PPAS medium on rhamnolipid production. Isolated rhamnolipids were characterized by IR and ESI-MS. IR spectra confirmed that isolated compound corresponds to rhamnolipid structure, whereas MS indicated that isolated preparation is a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic- and dirhamno- di-lipidic congeners.


RSC Advances ◽  
2015 ◽  
Vol 5 (42) ◽  
pp. 33089-33097 ◽  
Author(s):  
Hamidreza Hajfarajollah ◽  
Saeid Mehvari ◽  
Mahmoud Habibian ◽  
Babak Mokhtarani ◽  
Kambiz Akbari Noghabi

A glycolipid type of biosurfactant (rhamnolipid), which is obtained fromPseudomonas aeruginosaMA01, was adsorbed on a polypropylene film to produce an antimicrobial and antiadhesive polymeric surface for the first time.


2013 ◽  
Vol 68 (1-2) ◽  
pp. 47-52 ◽  
Author(s):  
Nelly Christova ◽  
Petar Petrov ◽  
Lyudmila Kabaivanova

Production of a rhamnolipid biosurfactant by cells of Pseudomonas aeruginosa strain BN10 immobilized into poly(ethylene oxide) (PEO) and polyacrylamide (PAAm) cryogels was investigated under semicontinuous shake fl ask conditions and compared to biosurfactant secretion by free cells. The biosurfactant synthesis was followed over 9 cycles of operation of the immobilized system, each cycle comprising 7 days at ambient temperature and neutral pH. Type and quantity of the carrier were optimized for the rhamnolipid production. The highest rhamnolipid yield of 4.6 g l-1 was obtained in the 6th cycle for the immobilized system with 3 g PEO compared to 4.2 g l-1 obtained for the free cells, thus immobilization provided physiological stability of the cells. Scanning electron microscopy revealed preservation of the cell shape and regular distribution of the cells under the matrix surface. The polymer matrices possessed chemical and biological stability and very good physico-mechanical characteristics which are a prerequisite for a high life span of these materials for the production of rhamnolipids


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