scholarly journals Removal of zinc and cadmium ions from contaminated soils with rhamnolipid biosurfactant produced by Pseudomonas aeruginosa S7PS5

2018 ◽  
Vol 8 (3) ◽  
pp. 1146
Author(s):  
M.E-A. Bendaha ◽  
B Meddah ◽  
H.A. Belaouni ◽  
M Mokhtar ◽  
A Tirtouil
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soils ◽  
Cadmium Ions ◽  
Rhamnolipid Biosurfactant

Production of Rhamnolipid Biosurfactant from Fed Batch Culture by Pseudomonas aeruginosa using Multiple Substrates

2020 ◽  
Vol 16 (6) ◽  
pp. 928-933
Author(s):  
Jujjavarapu S. Eswari
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Batch Process ◽  
Time Interval ◽  
Fed Batch ◽  
Multiple Substrates ◽  
Surface Active Agents ◽  
Limiting Nutrients ◽  
Limiting Nutrient ◽  
Surface Active ◽  
Rhamnolipid Biosurfactant

Objective: Biosurfactants are the surface active agents which are used for the reduction of surface and interfacial tensions of liquids. Rhamnolipids are the surfactants produced by Pseudomonas aeruginosa. It requires minimum nutrition for its growth as it can also grow in distilled water. The rhamnolipids produced by Pseudomonas aeruginosa are extra-cellular glycolipids consisting of L-rhamnose and 3-hydroxyalkanoic acid. Methods: The fed-batch method for the rhamnolipid production is considered in this study to know the influence of the carbon, nitrogen, phosphorous substrates as growth-limiting nutrients. Pulse feeding is employed for limiting nutrient addition at particular time interval to obtain maximum rhamnolipid formation from Pseudomonas aeruginosa compared with the batch process. Results: Out of 3 fed batch strategies constant glucose fed batch strategy shows best and gave maximum rhamnolipid concentration of 0.134 g/l.

scholarly journals Rhamnolipid Biosurfactant Production by Strains of Pseudomonas aeruginosa Using Low-Cost Raw Materials

2002 ◽  
Vol 18 (6) ◽  
pp. 1277-1281 ◽  
Author(s):  
K.S.M. Rahman ◽  
T.J. Rahman ◽  
S. McClean ◽  
R. Marchant ◽  
I.M. Banat
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Raw Materials ◽  
Low Cost ◽  
Biosurfactant Production ◽  
Rhamnolipid Biosurfactant

scholarly journals Production and characterization of rhamnolipids from Pseudomonas aeruginosa san ai

2012 ◽  
Vol 77 (1) ◽  
pp. 27-42 ◽  
Author(s):  
Milena Rikalovic ◽  
Gordana Gojgic-Cvijovic ◽  
Miroslav Vrvic ◽  
Ivanka Karadzic
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Response Surface Methodology ◽  
Nitrogen Source ◽  
Testing Effect ◽  
Carbon And Nitrogen ◽  
Rhamnolipid Production ◽  
Proteose Peptone ◽  
Isolated Compound ◽  
Rhamnolipid Biosurfactant

Production and characterization of rhamnolipid biosurfactant obtained by strain Pseudomonas aeruginosa san ai was investigated. With regard to carbon and nitrogen source several media were tested to enhance production of rhamnolipids. Phosphate-limited proteose peptone-ammonium salt (PPAS) medium supplemented with sun flower oil as a source of carbon and mineral ammonium chloride and peptone as a nitrogen source greatly improved rhamnolipid production, from 0.15 on basic PPAS (C/N ratio 4.0), to 3 g L-1, on optimized PPAS medium (C/N ratio 7.7). Response surface methodology analysis was used for testing effect of three factors: temperature, concentration of carbon and nitrogen source (w/w), in optimized PPAS medium on rhamnolipid production. Isolated rhamnolipids were characterized by IR and ESI-MS. IR spectra confirmed that isolated compound corresponds to rhamnolipid structure, whereas MS indicated that isolated preparation is a mixture of mono-rhamno-mono-lipidic, mono-rhamno-di-lipidic- and dirhamno- di-lipidic congeners.

Rhamnolipid biosurfactant adsorption on a plasma-treated polypropylene surface to induce antimicrobial and antiadhesive properties

RSC Advances ◽  
2015 ◽  
Vol 5 (42) ◽  
pp. 33089-33097 ◽  
Author(s):  
Hamidreza Hajfarajollah ◽  
Saeid Mehvari ◽  
Mahmoud Habibian ◽  
Babak Mokhtarani ◽  
Kambiz Akbari Noghabi
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Polypropylene Film ◽  
Polymeric Surface ◽  
Rhamnolipid Biosurfactant ◽  
First Time

A glycolipid type of biosurfactant (rhamnolipid), which is obtained fromPseudomonas aeruginosaMA01, was adsorbed on a polypropylene film to produce an antimicrobial and antiadhesive polymeric surface for the first time.

scholarly journals Screening of hydrocarbonoclastic bacteria using redox indicator 2, 6-dichlorophenol indophenol.

2015 ◽  
Vol 17 (3) ◽  
pp. 565-573 ◽  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Proteus Mirabilis ◽  
Contaminated Soils ◽  
Bacterial Species ◽  
Petroleum Products ◽  
Engine Oil ◽  
Toxic Substances ◽  
Hydrocarbonoclastic Bacteria ◽  
Bacterial Counts ◽  
Redox Indicator

<div> <p>Petroleum products are used in different forms in auto-mechanic workshops every day. Spent motor oil disposed-off improperly contains potentially toxic substances which can seep into the water tables and contaminate ground water. This study involved isolation and screening of bacterial species capable of utilizing hydrocarbons from three auto-mechanic workshops in Abeokuta, Ogun State. Total Heterotrophic Bacterial Counts ranged from 1.03 &times; 10<sup>6</sup> to 2.81 &times; 10<sup>6 </sup>CFU/g. Total Oil Degrading Bacterial Counts varied between 4.0 &times; 10<sup>5</sup> and 2.01 &times; 10<sup>6</sup> CFU/g while Surface Active Bacterial Counts were from 1.2 &times; 10<sup>4</sup> to 2.76 &times; 10<sup>5</sup> CFU/g. Twenty-four bacteria species capable of utilizing petroleum as a carbon source were isolated from various contaminated soils using enrichment technique. Isolated bacteria include: <em>Bacillus </em>spp., <em>Pseudomonas aeruginosa,</em> <em>Micrococcus </em>spp., <em>Proteus mirabilis</em>, <em>Proteus vulgaris </em>and<em> Enterobacter </em>spp. Redox indicator 2, 6-dichlorophenol indophenol (DCPIP) was used to screen for efficient hydrocarbon (Kerosene, Premium Motor Spirit, and Engine oil) degradation by bacteria. Degradation efficiency was measured by optical density at 600 nm. <em>Micrococcus </em>spp., <em>Proteus mirabilis</em>, and <em>Pseudomonas aeruginosa</em> were found to be the best isolates growing on majority of hydrocarbons due to their high utilization value when growing on the hydrocarbons tested.</p> </div> <p>&nbsp;</p>

Интенсификация биоремедиации нефтезагрязненных почв с использованием природного консорциума микроорганизмов

2021 ◽  
pp. 582-589
Author(s):  
Kalamkas B. Oralbayeva ◽  
Aliya K. Yernazarova ◽  
Medet E. Kundybayev ◽  
Spandiar A. Seitkulov ◽  
Aida S. Kistaubayeva
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Climatic Conditions ◽  
Mineral Fertilizers ◽  
Pseudomonas Sp ◽  
Bacterial Strains ◽  
Biological Product ◽  
Metabolic Potential ◽  
Alcaligenes Denitrificans

Currently biological methods of cleaning oily soils significantly developed as the most accessible and effective. In their basis – the use of hydrocarbon-oxidizing microorganisms (HOM) metabolic potential. At the same time, as practice shows, existing preparations containing HOM are not effective enough in extreme soil-climatic conditions, in this connection the search for indigenous strains of oil-oxidizing bacteria and the development of new biological preparations are of current interest. The aim of this work is to study the effectiveness of HOM strains for restoring the biological activity of oil-contaminated soil. A study of the microflora of unpolluted and oil-contaminated soil taken from the experimental site of the “Shymkent” oil pumping station of “KazTransOil” JSC was carried out: the total number of microorganisms was 210·106cells/g and 130·106 cells/g, respectively. From 16 НOM strains isolated from oil-contaminated soil, 3 bacterial strains were selected to create an association and use as a biological product-oil destructor – P12, P16, A8. Based on the results of genotyping, strains P12 and P16 correspond to the species Pseudomonas aeruginosa, strain A8 belongs to the species Alcaligenes denitrificans. The associations of Pseudomonas sp. P16, Pseudomonas sp. P12 and Alcaligenes sp. A8 showed better growth in comparison with monocultures on a nutrient medium with hydrocarbons. Bioremediation of artificially oil polluted site process, which carried out for 120 days using a biological product created on the basis of the association of strains P12, P16 and A8, decreased the content of hydrocarbons 3.4 times, and with additional application of mineral fertilizers (ammophos) – 4.6 times. Thus, proved the expediency of using HOM strains P12, P16, A8 for the purpose of bioremediation of oil-contaminated soils. The results of the study can be used to improve economical and environmental efficiency of work when carrying out measures to eliminate the consequences of oil spills on the ground. Биологические методы очистки нефтесодержащих почв в настоящее время имеют существенное развитие как наиболее доступные и эффективные. В их основе – использование метаболического потенциала углеводородокисляющих микроорганизмов (УОМ). При этом, как показывает практика, существующие препараты, содержащие УОМ, недостаточно эффективны в экстремальных почвенно-климатических условиях, в связи с чем актуальными являются вопросы поиска аборигенных штаммов нефтеокисляющих бактерий и разработка новых биопрепаратов. Цель работы – исследование эффективности штаммов УОМ для восстановления биологической активности нефтезагрязненного почвогрунта. Проведено изучение микрофлоры незагрязненной и нефтезагрязненной почвы, отобранной с экспериментального участка ГНПС «Шымкент» АО «КазТрансОйл»: общее количество микроорганизмов составило 210·106 кл/г и 130·106кл/г, соответственно. Из выделенных из нефтезагрязненной почвы 16 штаммов УОМ были отобраны три штамма бактерий для создания ассоциации и применения в качестве биопрепарата-нефтедеструктора – Р12, Р16, А8. По итогам генотипирования установлено, что штаммы Р12 и Р16 соответствуют виду Pseudomonas aeruginosa, штамм А8 относится к виду Alcaligenes denitrificans. Установлено, что в составе ассоциаций штаммы Pseudomonas sp. P16, Pseudomonas sp. P12 и Alcaligenes sp. A8. показали лучший по сравнению с монокультурами рост на питательной среде с углеводородами. В процессе биоремедиации участка с искусственным нефтезагрязнением, проводимой в течение 120 суток с использованием биопрепарата, созданного на основе ассоциации штаммов P12, P16 и A8, содержание углеводородов снизилось в 3,4 раза, а при дополнительном внесении минеральных удобрений (аммофоса) – в 4,6 раза. Таким образом, доказана целесообразность использования штаммов УОМ Р12, Р16, А8 с целью биоремедиации нефтезагрязненных почв. Результаты исследования могут быть использованы для повышения экономической и экологической эффективности работ при проведении мероприятий по ликвидации последствий разливов нефти на почве.

Sign in / Sign up

Export Citation Format

Share Document