scholarly journals W48. EFFICIENT HIPSC BASED DISEASE MODELLING USING CRISPR/CAS9

2021 ◽  
Vol 51 ◽  
pp. e169-e170
Author(s):  
Atefeh Namipashaki ◽  
Xiaodong Liu ◽  
Kealan Pugsley ◽  
Jose M. Polo ◽  
Mark Bellgrove ◽  
...  
Keyword(s):  
2020 ◽  
Vol 88 ◽  
pp. S55
Author(s):  
Yelena Boccacci ◽  
Guillaume Margaillan ◽  
Nellie Dumont ◽  
Mathieu Drouin ◽  
Yannick Doyon ◽  
...  

2018 ◽  
Vol 12 (supplement_1) ◽  
pp. S553-S553
Author(s):  
D Kurek ◽  
E Naumowska ◽  
S Trietsch ◽  
M Setyawati ◽  
K Wilschut ◽  
...  
Keyword(s):  

2018 ◽  
Vol 15 (2) ◽  
pp. 276-285 ◽  
Author(s):  
Lisa Hinz ◽  
Stephanie D. Hoekstra ◽  
Kyoko Watanabe ◽  
Danielle Posthuma ◽  
Vivi M. Heine

Development ◽  
2021 ◽  
Vol 148 (4) ◽  
pp. dev180612
Author(s):  
Filip J. Wymeersch ◽  
Valerie Wilson ◽  
Anestis Tsakiridis

ABSTRACTThe generation of the components that make up the embryonic body axis, such as the spinal cord and vertebral column, takes place in an anterior-to-posterior (head-to-tail) direction. This process is driven by the coordinated production of various cell types from a pool of posteriorly-located axial progenitors. Here, we review the key features of this process and the biology of axial progenitors, including neuromesodermal progenitors, the common precursors of the spinal cord and trunk musculature. We discuss recent developments in the in vitro production of axial progenitors and their potential implications in disease modelling and regenerative medicine.


2020 ◽  
Author(s):  
Amar M. Singh ◽  
Liang Zhang ◽  
John Avery ◽  
Stephen Dalton

Abstract Beige adipocytes (also known as brite adipocytes) have significant utility for numerous applications, such as drug screening, cell therapy, and disease modelling. However, a high-efficiency protocol from human adult adipose-derived stem/stromal stem cells (ADSC) has not been described. The protocol described here achieves beige adipocyte purities of >92% in a fully-defined, serum-free media cocktail, which enables these downstream applications. This method provides a significant leap forward over previously described, serum-based protocols that were inconsistent and inefficient.


2021 ◽  
Author(s):  
Zulqurnain Sabir ◽  
Muhammad Asif Zahoor Raja ◽  
Mohamed R. Ali ◽  
Adnène Arbi ◽  
Muhammad Kristiawan

Abstract In this study, an advanced computational numerical scheme based on the Levenberg-Marquardt backpropagation (LMB) neural network (NN) process, i.e., LMB-NN is presented for solving the nonlinear mathematical influenza disease model. The nonlinear mathematical influenza disease model depends on four categories named susceptible S(t), infected I(t), recovered R(t) and cross-immune individuals proportion C(t). Six different cases of the nonlinear mathematical influenza disease model have been numerically treated using the LMB-NN process and the comparison of the results has been presented by using the reference data-based solutions designed based on the Adams results. The numerically obtained results of the nonlinear mathematical influenza disease model using the verification, testing, and training procedures are calculated using the LMB-NN process to reduce the functions of mean square error (MSE). For the correctness, competence, effectiveness, and efficiency of the LMB-NN process, the proportional and analysis methods are performed using the analysis of correlation, MSE results, error histograms and regression.


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