Deletion of beaded filament proteins or the C-terminal end of Aquaporin 0 causes analogous abnormal distortion aberrations in mouse lens

The nude mouse is a hairless mutant (homozygous for the mutation nude, nu/nu), which is born lacking a thymus and possesses a severe defect in cellular immunity. Spontaneous unilateral cataractous lesions were noted (during ocular examination using a stereomicroscope at 40X) in 14 of a series of 60 animals (20%). This transmission and scanning microscopic study characterizes the morphology of this cataract and contrasts these data with normal nude mouse lens.All animals were sacrificed by an ether overdose. Eyes were enucleated and immersed in a mixed fixative (1% osmium tetroxide and 6% glutaraldehyde in Sorenson's phosphate buffer pH 7.4 at 0-4°C) for 3 hours, dehydrated in graded ethanols and embedded in Epon-Araldite for transmission microscopy. Specimens for scanning electron microscopy were fixed similarly, dehydrated in graded ethanols, then to graded changes of Freon 113 and ethanol to 100% Freon 113 and critically point dried in a Bomar critical point dryer using Freon 13 as the transition fluid.

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The labile nature of the insulin signal(s) for the stimulation of DNA synthesis in mouse lens epithelial and 3T3 cells.

Journal of Biological Chemistry ◽

10.1016/s0021-9258(19)75915-8 ◽

1987 ◽

Vol 262 (1) ◽

pp. 229-233

Author(s):

T W Reid ◽

W A Reid

Keyword(s):

Dna Synthesis ◽

3T3 Cells ◽

Insulin Signal ◽

Mouse Lens ◽

Stimulation Of

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Mitomycin C-Induced Cell Death in Mouse Lens Epithelial Cells

Ophthalmic Research ◽

10.1159/000063880 ◽

2002 ◽

Vol 34 (4) ◽

pp. 213-219 ◽

Cited By ~ 10

Author(s):

Hyun-Kyung Park ◽

Kwang-Won Lee ◽

Jun-Sub Choi ◽

Choun-Ki Joo

Keyword(s):

Cell Death ◽

Epithelial Cells ◽

Mitomycin C ◽

Lens Epithelial Cells ◽

Mouse Lens

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A Hierarchy of Proliferative Cells Exists in Mouse Lens Epithelium: Implications for Lens Maintenance

Investigative Opthalmology & Visual Science ◽

10.1167/iovs.06-0130 ◽

2006 ◽

Vol 47 (7) ◽

pp. 2997 ◽

Cited By ~ 34

Author(s):

Mingyuan Zhou ◽

Joshua Leiberman ◽

Jing Xu ◽

Robert M. Lavker

Keyword(s):

Lens Epithelium ◽

Mouse Lens

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Actin filament patterns in mouse lens epithelium: A study of the effects of aging, injury, and genetics

Cell Motility and the Cytoskeleton ◽

10.1002/cm.970090104 ◽

1988 ◽

Vol 9 (1) ◽

pp. 17-29 ◽

Cited By ~ 14

Author(s):

Willisa Liou ◽

Nancy S. Rafferty

Keyword(s):

Actin Filament ◽

Lens Epithelium ◽

Effects Of Aging ◽

Mouse Lens

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Effect of exogenous stress on the tissue-cultured mouse lens epithelial cells

Journal of Cellular Biochemistry ◽

10.1002/jcb.10224 ◽

2002 ◽

Vol 86 (2) ◽

pp. 302-306 ◽

Cited By ~ 4

Author(s):

Mihir Bagchi ◽

Malkhan Katar ◽

H. Maisel

Keyword(s):

Epithelial Cells ◽

Lens Epithelial Cells ◽

Exogenous Stress ◽

Mouse Lens

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Rescue of developmental lens abnormalities in chimaeras of noncataractous and congenital cataractous mice

Development ◽

10.1242/dev.99.4.473 ◽

1987 ◽

Vol 99 (4) ◽

pp. 473-480

Author(s):

A.L. Muggleton-Harris ◽

K. Hardy ◽

N. Higbee

Keyword(s):

Medical Research ◽

Congenital Cataract ◽

Biochemical Analysis ◽

Growth Regulation ◽

Cellular Level ◽

Model System ◽

Mouse Mutant ◽

Phenotypic Characteristics ◽

Cataractous Lens ◽

Mouse Lens

In the study of the lens of a congenital cataractous mouse mutant (CAT), it has been shown that a loss of growth regulation at the cellular level causes gross lens abnormalities. The phenotypic characteristics of the cataractous mouse lens are similar to those seen in human congenital cataract and thus serves as a model system for medical research. In this present investigation, we have demonstrated that the abnormalities of the congenital cataractous lens can be rescued by forming chimaeras between DBA/2 (a noncataractous strain of mouse) and the CAT mutant. This report describes the histological, cellular and biochemical analysis of the resultant chimaeric eyes, and discusses possible mechanisms by which these results were achieved.

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