Changes in secretory cell turnover, and mitochondrial oxidative damage in the mouse mammary gland during a single prolonged lactation cycle suggest the possibility of accelerated cellular aging

2006 ◽  
Vol 41 (3) ◽  
pp. 271-281 ◽  
Author(s):  
D HADSELL ◽  
D TORRES ◽  
J GEORGE ◽  
A CAPUCO ◽  
S ELLIS ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Oxidative Damage ◽  
Secretory Cell ◽  
Mouse Mammary Gland ◽  
Cellular Aging ◽  
Cell Turnover ◽  
Mitochondrial Oxidative Damage ◽  
Lactation Cycle

Sensitivity of mammary secretory cell turnover to protein restriction and re-alimentation during early lactation

1997 ◽  
Vol 1997 ◽  
pp. 130-130
Author(s):  
M.G. Goodwill ◽  
N.S. Jessop ◽  
J.D. Oldham
Keyword(s):  
Cell Proliferation ◽  
Mammary Gland ◽  
Milk Production ◽  
Secretory Cell ◽  
Protein Restriction ◽  
Secretory Cells ◽  
Early Lactation ◽  
Cell Turnover ◽  
Lactational Performance

Milk production depends on both the number and activity of secretory cells within the mammary gland. Our earlier work showed the sensitivity of lactational performance to changes in diet during lactation (Goodwill et al, 1996). This study investigated the influence of protein undernutrition and re-alimentation on secretory cell proliferation and death in the mammary gland of rats during early lactation.

Integrity of the secretory epithelium of the lactating mouse mammary gland during extended periods after suckling

1997 ◽  
Vol 64 (1) ◽  
pp. 39-45 ◽  
Author(s):  
YURI A. TOLKUNOV ◽  
ALEXANDER G. MARKOV
Keyword(s):  
Mammary Gland ◽  
Secretory Cell ◽  
Mouse Mammary Gland ◽  
Ionic Concentration ◽  
Potential Difference ◽  
Phase Reaction ◽  
Concentration Gradients ◽  
Initial Value ◽  
Short Term ◽  
Two Phase

The transepithelial potential difference (TEPD) and resistance (TER) in the alveoli, and the pressure in the mammary gland were measured in lactating mice at different times after they had finished suckling their young. Oxytocin administration caused a rise in TEPD, the amplitude of which decreased with time after cessation of the pups feeding. At 2·5 h after cessation, oxytocin administration caused a short-term decrease in TER, but at 5 h there was a two phase reaction: a fall in TER followed by a rise. At 20 h there was a one phase reaction, a rise in TER. TEPD in the mammary gland alveoli increased from its initial value of 18±1 mV to 25±1 mV at 2·5 h, and thereafter fell to zero by 15 h after suckling. The initial value of TER, 114±1 kΩ, increased to 131±4 kΩ at 2·5 h and then decreased by 5 h after suckling, but unlike TEPD it did not reach zero. These experiments show that extending the period after cessation of suckling does not cause a rise in intramammary pressure and that oxytocin injections cause a short-term rise in milk pressure. From these results, we propose a hypothesis of reducing ionic concentration gradients between intracellular fluid, cytoplasm of the secretory cell and milk at extended periods after cessation of suckling, starting at 2·5 h.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

mRNA expression of lipogenic genes in mouse mammary gland in different lactation stages

2012 ◽  
Vol 34 (3) ◽  
pp. 335-341
Author(s):  
Li-Qiang HAN ◽  
Hong-Ji LI ◽  
Yue-Ying WANG ◽  
Lin-Feng WANG ◽  
Guo-Qing YANG ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Mouse Mammary Gland ◽  
Lipogenic Genes
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