Acaricidal activity of petroleum ether extract of neem (Azadirachta indica) oil and its four fractions separated by column chromatography against Sarcoptes scabiei var. cuniculi larvae in vitro

2012 ◽  
Vol 130 (4) ◽  
pp. 475-477 ◽  
Author(s):  
Yunxia Deng ◽  
Dongxia Shi ◽  
Zhongqiong Yin ◽  
Jianhong Guo ◽  
Renyong Jia ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Column Chromatography ◽  
Azadirachta Indica ◽  
Petroleum Ether Extract ◽  
Sarcoptes Scabiei ◽  
Acaricidal Activity ◽  
Ether Extract

scholarly journals PHYTOCHEMICAL INVESTIGATION AND IN VITRO ANTIMICROBIAL SCREENING OF SANTALUM ALBUM SEEDS EXTRACTS

2017 ◽  
Vol 9 (10) ◽  
pp. 117
Author(s):  
Gautam P. Vadnere ◽  
Md. Rageeb Usman ◽  
Santram Lodhi ◽  
Vaishali Patil
Keyword(s):  
Antimicrobial Activity ◽  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Santalum Album ◽  
Ether Extract ◽  
Gram Positive ◽  
Gram Negative ◽  
Antimicrobial Screening ◽  
Ethanol Extracts

Objective: Present study aimed phytochemical evaluation and antimicrobial screening of petroleum ether and ethanol extracts of Santalum album seeds.Methods: Petroleum ether and ethanol extracts were screened for presence of chemical constituents. Petroleum ether extract was investigated detail by using chromatographic and spectroscopic methods. In vitro antimicrobial activity of both extracts were investigated using disc diffusion method on two gram-positive bacteria, Bacillus subtilis, Staphylococcus aureus, gram negative Pseudomonas aeruginosa, Escherichia coli and fungus Candida albicans.Results: Santalbic acid was identified in petroleum ether extract and content determined by HPTLC was 4.7%w/w. It was seen that petroleum ether extract have MIC value for B. subtilis, P. aeruginosa, E. coli and C. albicans were 78.125 µg/ml, 19.331 µg/ml, 625 µg/ml & 39.062 µg/ml respectively while MBC was 39.062 µg/ml, 4.882 µg/ml, 312.5 µg/ml & 9.765 µg/ml,  respectively. Petroleum ether extract showed MIC and MBC values for S. aureus was similar as 156.25µg/ml. So, the petroleum ether extract showed significant antimicrobial activity against both gram positive, gram negative and fungal strain.Conclusions: The results of present investigations were indicative of possible high potency of petroleum ether extract due to santalbic acid which could serve as chemotherapeutic agent.

scholarly journals In vitro Phytochemical Investigation of Helianthus annuus Seeds

2016 ◽  
Vol 19 (1) ◽  
pp. 100-105 ◽  
Author(s):  
Rubab Tarannum Islam ◽  
Mir Monir Hossain ◽  
Kishor Majumder ◽  
Amjad Hossain Tipu
Keyword(s):  
Petroleum Ether ◽  
Helianthus Annuus ◽  
Biological Activities ◽  
Petroleum Ether Extract ◽  
Radical Scavenging ◽  
High Sensitivity ◽  
Chemical Components ◽  
Phytochemical Analysis ◽  
Ether Extract

The seeds of Helianthus annuus have been investigated for the presence of various chemical components and biological activities with special emphasis to the anti-oxidant, antimicrobial, cytotoxic and thrombolytic assay. The results of standard phytochemical analysis revealed the presence of tannins, saponins, flavonoids, carbohydrates, steroids, fixed oils and vitamins. In vitro antioxidant activity of methanolic seed extract of H. annuus was determined by DPPH free radical scavenging assay, which demonstrated very significant antioxidant potential compared to standard antioxidant (ascorbic acid). In case of antimicrobial assay, the methanolic and ethyl acetate extract of H. annuus seeds showed high sensitivity to Salmonella typhi and moderate sensitivity to Pseudomonous aeuregenosa. The petroleum ether extract revealed high sensitivity to P. aeuregenosa. The methanolic extract of H. annuus displayed LC50 1.2 ug/ml and its petroleum ether extract showed LC50 of 1.1 ug/ml, which indicated significant cytotoxicity.Bangladesh Pharmaceutical Journal 19(1): 100-105, 2016

scholarly journals Acaricidal Activity of Petroleum Ether Extract of Leaves ofTetrastigma leucostaphylum(Dennst.) Alston againstRhipicephalus (Boophilus) annulatus

2014 ◽  
Vol 2014 ◽  
pp. 1-6 ◽  
Author(s):  
T. P. Adarsh Krishna ◽  
T. P. Ajeesh Krishna ◽  
N. D. Chithra ◽  
P. E. Deepa ◽  
U. Darsana ◽  
...  
Keyword(s):  
Petroleum Ether ◽  
Petroleum Ether Extract ◽  
Coconut Oil ◽  
Adult Tick ◽  
Immersion Test ◽  
Adult Mortality ◽  
Acaricidal Activity ◽  
Ether Extract ◽  
Boophilus Annulatus ◽  
Additive Action

The acaricidal activity of the petroleum ether extract of leaves ofTetrastigma leucostaphylum(Dennst.) Alston (family: Vitaceae) againstRhipicephalus (Boophilus) annulatuswas assessed using adult immersion test (AIT). The per cent of adult mortality, inhibition of fecundity, and blocking of hatching of eggs were studied at different concentrations. The extract at 10% concentration showed 88.96% inhibition of fecundity, 58.32% of adult tick mortality, and 50% inhibition of hatching. Peak mortality rate was observed after day 5 of treatment. Mortality of engorged female ticks, inhibition of fecundity, and hatching of eggs were concentration dependent. The LC50value of the extract againstR. (B.) annulatuswas 10.46%. The HPTLC profiling of the petroleum ether extract revealed the presence of at least seven polyvalent components. In the petroleum ether extract, nicotine was identified as one of the components up to a concentration of 5.4%. However, nicotine did not reveal any acaricidal activity up to 20000 ppm (2%). Coconut oil, used as diluent for dissolving the extract, did not reveal any acaricidal effects. The results are indicative of the involvement of synergistic or additive action of the bioactive components in the tick mortality and inhibition of the oviposition.

Acaricidal activity of extracts of neem (Azadirachta indica) oil against the larvae of the rabbit mite Sarcoptes scabiei var. cuniculi in vitro

2008 ◽  
Vol 157 (1-2) ◽  
pp. 144-148 ◽  
Author(s):  
Yong-Hua Du ◽  
Ren-Yong Jia ◽  
Zhong-Qiong Yin ◽  
Zhong-Hui Pu ◽  
Jiao Chen ◽  
...  
Keyword(s):  
Azadirachta Indica ◽  
Sarcoptes Scabiei ◽  
Acaricidal Activity

scholarly journals IN VITRO ANTIMALARIAL ACTIVITY OF LEAVE PETROLEUM ETHER EXTRACT OF FICUS SYCOMORUS PLANT

2021 ◽  
Vol 8 (12) ◽  
pp. 279-283
Author(s):  
M. A. Garga ◽  
M. K. Ibrahim ◽  
A. Mohammad ◽  
H. A. Abdullahi ◽  
M. B. Yusuf ◽  
...  
Keyword(s):  
Growth Inhibition ◽  
Petroleum Ether ◽  
Antimalarial Activity ◽  
Petroleum Ether Extract ◽  
Ether Extract ◽  
Ficus Sycomorus

In vitro anti-malarial activity of petroleum ether extracts of Ficus sycomorus leaves was investigated using Candle Jar method. The extractions were carried out through activity guided fractionation. Petroleum ether extract has the highest activity, at the concentration of 1mg and 0.5mg with percentage growth inhibition of (33.12 and 31.9%) respectively. These results suggest that the leaves of Ficus sycomorus plant have significant antimalarial activity and that the antiplasmodial agents might be as a result of the extract fractionated using the petroleum.

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