Expeditious production of concentrated glucose-rich hydrolysate from sugarcane bagasse and its fermentation to lactic acid with high productivity

Delignified sugarcane bagasse (SCB) by solvent extraction was carried out at moderate condition (90°C, 4 h) in the presence of acid catalyst. To investigate the dissolution of lignin into solvent, different solvents were utilized during lignin extraction process. Delignified SCB was further hydrolyzed by cell wall degrading enzyme complexes prior to sugar determination. The results showed that n-butanol was the most promising solvent enhancing lignin dissolution which in turn led to highest yield of glucose (63.1% based on treated SCB) and no xylose was detected in hydrolysate. The lignin extraction by n-butanol in the presence of H2SO4 and subsequent saccharification process were then scaled up for lactic acid production. The maximum lactic acid was obtained at 25.7 ± 0.2 g/l from L. casei fermentation after 96 hours when sugarcane bagasse hydrolysate containing 25.6±1.4 g/l initial glucose concentration was used as substrate.

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Mixed morphology nanocrystalline cellulose from sugarcane bagasse fibers/poly(lactic acid) nanocomposite films: synthesis, fabrication and characterization

Iranian Polymer Journal ◽

10.1007/s13726-017-0504-6 ◽

2017 ◽

Vol 26 (2) ◽

pp. 125-136 ◽

Cited By ~ 4

Author(s):

Arun. M. Panicker ◽

K. A. Rajesh ◽

T. O. Varghese

Keyword(s):

Lactic Acid ◽

Sugarcane Bagasse ◽

Nanocrystalline Cellulose ◽

Nanocomposite Films ◽

Poly Lactic Acid ◽

Fabrication And Characterization

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Replacement of a Metabolic Pathway for Large-Scale Production of Lactic Acid from Engineered Yeasts

Applied and Environmental Microbiology ◽

10.1128/aem.65.9.4211-4215.1999 ◽

1999 ◽

Vol 65 (9) ◽

pp. 4211-4215 ◽

Cited By ~ 98

Author(s):

Danilo Porro ◽

Michele M. Bianchi ◽

Luca Brambilla ◽

Rossella Menghini ◽

Davide Bolzani ◽

...

Keyword(s):

Lactic Acid ◽

Large Scale ◽

Kluyveromyces Lactis ◽

Lactic Acid Production ◽

Pyruvate Decarboxylase ◽

Polymer Materials ◽

Efficient Production ◽

Scale Production ◽

High Productivity ◽

Large Scale Production

ABSTRACT Interest in the production of l-(+)-lactic acid is presently growing in relation to its applications in the synthesis of biodegradable polymer materials. With the aim of obtaining efficient production and high productivity, we introduced the bovinel-lactate dehydrogenase gene (LDH) into a wild-type Kluyveromyces lactis yeast strain. The observed lactic acid production was not satisfactory due to the continued coproduction of ethanol. A further restructuring of the cellular metabolism was obtained by introducing the LDH gene into aK. lactis strain in which the unique pyruvate decarboxylase gene had been deleted. With this modified strain, in which lactic fermentation substituted completely for the pathway leading to the production of ethanol, we obtained concentrations, productivities, and yields of lactic acid as high as 109 g liter−1, 0.91 g liter−1 h−1, and 1.19 mol per mole of glucose consumed, respectively. The organic acid was also produced at pH levels lower than those usual for bacterial processes.

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Development of Fermented Chicken Sauce using Lactic Acid Bacteria with High Productivity of Gamma-Aminobutyric Acid

JOURNAL OF THE BREWING SOCIETY OF JAPAN ◽

10.6013/jbrewsocjapan.108.652 ◽

2013 ◽

Vol 108 (9) ◽

pp. 652-659

Author(s):

Taku MIYAMOTO ◽

Kensuke ARAKAWA ◽

Takayoshi KURIKI

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Aminobutyric Acid ◽

Gamma Aminobutyric Acid ◽

High Productivity

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